CAJ | 학술논문

目的建立鼠疫耶尔森菌和假结核耶尔森菌基因鉴别方法.方法依据鼠疫菌、假结核菌特有的基因组序列[“疫岛(PeI)”和“假岛(PsI)”],与已公布的12株鼠疫菌和4株假结核菌全基因序列进行比对,设计特异性的引物,对鼠疫菌、假结核菌和其他肠道细菌进行鉴定.结果用52株鼠疫菌、57株假结核菌和其他肠道菌株进行验证,结果显示,5对鼠疫菌的鉴定引物中,2对(PeI2和PeI11)仅在52株鼠疫菌中扩出目的条带,另3对引物(PeI1、PeI3和PeI12)除鼠疫菌外在部分假结核菌株中也扩出目的条带;5对假结核菌鉴定引物中,1对引物(PsI1)在52株鼠疫菌和57株假结核菌株中扩出目的条带,4对引物(PsI7、PsI16、PsI18和PsI19)仅在57株假结核菌株中均扩出目的条带,在鼠疫菌中未扩出目的条带.结论用鼠疫菌和假结核菌共有的PsI1序列、鼠疫菌特有的PeI2和PeI11序列及假结核菌特有的PsI7、PsI16、PsI18和PsI19序列组成的基因鉴别方法,可以用于鼠疫菌和假结核菌的基因快速鉴别.
목적 건립서역야이삼균화가결핵야이삼균기인감별방법.방법 의거서역균、가결핵균특유적기인조서렬[“역도(PeI)”화“가도(PsI)”],여이공포적12주서역균화4주가결핵균전기인서렬진행비대,설계특이성적인물,대서역균、가결핵균화기타장도세균진행감정.결과 용52주서역균、57주가결핵균화기타장도균주진행험증,결과현시,5대서역균적감정인물중,2대(PeI2화PeI11)부재52주서역균중확출목적조대,령3대인물(PeI1、PeI3화PeI12)제서역균외재부분가결핵균주중야확출목적조대;5대가결핵균감정인물중,1대인물(PsI1)재52주서역균화57주가결핵균주중확출목적조대,4대인물(PsI7、PsI16、PsI18화PsI19)부재57주가결핵균주중균확출목적조대,재서역균중미확출목적조대.결론 용서역균화가결핵균공유적PsI1서렬、서역균특유적PeI2화PeI11서렬급가결핵균특유적PsI7、PsI16、PsI18화PsI19서렬조성적기인감별방법,가이용우서역균화가결핵균적기인쾌속감별.
Objective To establish a gene identification method of Yersinia pestis and Yersinia pseudotuberculosis for plague surveillance.Methods According to the specific genomic sequences of Y.pestis and Y.pseudotuberculosis,i.e."pestis Island (PeI) " and "pseudotuberculosis Island (PsI)" and the published genomic sequences of 12 strains of Y.pestis and 4 strains of Y.pseudotuberculosis,the specific identification primers of these sequences were designed.Results A total of 52 strains of Y.pestis and 57 strains of Y.pseudotuberculosis and other intestinal bacteria strains were tested with PCR.Of the 5 pairs of Y.pestis identification primers,PeI2 and PeI11 were specific for Y.pestis.Besides Y.pestis,the primers PeI1,PeI3 and PeI12 could detect part of 57 Y.pseudotuberculosis strains.Of the 5 pairs of Y.pseudotuberculosis identification primers,PsI1 could detect all the 52 strains ofY.pestis and 57 strains of Y.pseudotuberculosis.PsI7,PsI16,PsI18 and PsI19 were specific for Y.pseudotuberculosis.Conclusion The primers PsI1,PeI 2 and PeI11,PsI7,PsI16,PsI18 and PsI19 can be used in the rapid identification of Y.pestis and Y.pseudotuberculosis,which can be also used to explore the circulation of atypical Y.pestis in quiescent plague foci.