CAJ | 학술논문

目的探讨新疆地区维吾尔族和汉族妇女乳腺癌患者中环氧合酶-2(COX-2)基因在mRNA水平表达差异.方法收集新疆医科大学附属肿瘤医院详尽的乳腺癌病例资料及新鲜标本120例,应用实时荧光定量聚合酶链反应(FQ-PCR)技术检测癌、癌旁及纤维腺瘤组织中COX-2基因在mRNA表达水平,了解其在维、汉组间及组内表达差异.结果 41例汉族癌、8例癌旁及16例良性组织中COX-2表达水平分别为1.71(0.71 ～6.83)、1.25(0.76 ～ 1.39)、0.42(0.15 ～0.93);与良性对照组比较,COX-2在癌组织中表达明显升高,差异有统计学意义(P＜0.01);38例维吾尔族癌、13例癌旁及4例良性组织中COX-2基因表达水平分别为0.41(0.12 ～2.35)、0.95(0.45 ～2.17)、1.33(0.32 ～3.21),与对照组比较,尚未见COX-2基因在癌组织中表达升高(P＞0.05);维、汉两组癌组织比较差异有统计学意义(P＜0.01).结论乳腺癌中COX-2基因表达较良性组织明显增高,维吾尔族和汉族COX-2表达有种族差异.
목적 탐토신강지구유오이족화한족부녀유선암환자중배양합매-2(COX-2)기인재mRNA수평표체차이.방법 수집신강의과대학부속종류의원상진적유선암병례자료급신선표본120례,응용실시형광정량취합매련반응(FQ-PCR)기술검측암、암방급섬유선류조직중COX-2기인재mRNA표체수평,료해기재유、한조간급조내표체차이.결과 41례한족암、8례암방급16례량성조직중COX-2표체수평분별위1.71(0.71 ～6.83)、1.25(0.76 ～ 1.39)、0.42(0.15 ～0.93);여량성대조조비교,COX-2재암조직중표체명현승고,차이유통계학의의(P＜0.01);38례유오이족암、13례암방급4례량성조직중COX-2기인표체수평분별위0.41(0.12 ～2.35)、0.95(0.45 ～2.17)、1.33(0.32 ～3.21),여대조조비교,상미견COX-2기인재암조직중표체승고(P＞0.05);유、한량조암조직비교차이유통계학의의(P＜0.01).결론 유선암중COX-2기인표체교량성조직명현증고,유오이족화한족COX-2표체유충족차이.
Objective To investigate the differential expression of cyclooxygenase-2 (COX-2)mRNA in Uygur and Han women with breast cancer in Xinjiang area.Methods Xinjiang Medical University affiliated Tumor Hospital collect detailed information on breast cancer cases and 120 cases of fresh specimens were detected by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR)carcinoma,paraneoplastic and fibroadenoma tissue COX-2 gene expression at the mRNA level,analysis of its expression differences between groups and within groups of Han and Uygur.Results 41 cases of Han cancer,8 cases of paraneoplastic and 16 cases of benign tissue,COX-2 expression levels were 1.71 (0.71 to6.83),1.25 (0.76 to 1.39),0.42 (0.15 to 0.93) respectively;compared with the control group,COX 2 expression was significantly higher in cancer tissues,the difference was statistically significant (P ＜0.01);38 cases of Uygur cancer,13 cases of paraneoplastic and four cases of benign tissue,COX-2 expression levels were 0.41 (0.12 to 2.35),0.95 (0.45 to 2.17),1.33 (0.32 to 3.21) respectively,compared with the control group,yet see COX-2 expression in cancer tissues increased (P ＞0.05);Uygur and Han carcinoma groups difference was statistically significant (P ＜ 0.01).Conclusion COX-2 expression in breast cancer was significantly higher compared with benign tissue,Uygur and Han COX-2 expression was statistically significant,suggesting that COX-2 expression of ethnic differences.