中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
5期
1006-1008
,共3页
骨髓%树突状细胞%细胞培养%鉴定
骨髓%樹突狀細胞%細胞培養%鑒定
골수%수돌상세포%세포배양%감정
Bone marrow%Dendritic cells%Cell culture%Identification
目的 鉴定体外诱导培养大鼠树突状细胞(DCs)的形态.方法 利用细胞因子体外诱导和大量扩增大鼠骨髓来源的DCs,并采用苏木素-伊红(HE)染色、巴氏染色和Diff-Quick染色进行形态学鉴定,采用免疫组织化学及免疫荧光染色方法检测细胞表面标志物OX62和CD86分子对DCs进行表型鉴定.结果 DCs表面有突起,胞核不规则,胞质可见吞噬小泡.成熟DCs(mDCs)和不成熟DCs(imDCs)表面均表达OX62分子,imDCs低表达CD86分子,而mDCs表面高表达CD86分子.结论 建立在体外大量扩增和多种鉴定大鼠骨髓来源DCs的方法.
目的 鑒定體外誘導培養大鼠樹突狀細胞(DCs)的形態.方法 利用細胞因子體外誘導和大量擴增大鼠骨髓來源的DCs,併採用囌木素-伊紅(HE)染色、巴氏染色和Diff-Quick染色進行形態學鑒定,採用免疫組織化學及免疫熒光染色方法檢測細胞錶麵標誌物OX62和CD86分子對DCs進行錶型鑒定.結果 DCs錶麵有突起,胞覈不規則,胞質可見吞噬小泡.成熟DCs(mDCs)和不成熟DCs(imDCs)錶麵均錶達OX62分子,imDCs低錶達CD86分子,而mDCs錶麵高錶達CD86分子.結論 建立在體外大量擴增和多種鑒定大鼠骨髓來源DCs的方法.
목적 감정체외유도배양대서수돌상세포(DCs)적형태.방법 이용세포인자체외유도화대량확증대서골수래원적DCs,병채용소목소-이홍(HE)염색、파씨염색화Diff-Quick염색진행형태학감정,채용면역조직화학급면역형광염색방법검측세포표면표지물OX62화CD86분자대DCs진행표형감정.결과 DCs표면유돌기,포핵불규칙,포질가견탄서소포.성숙DCs(mDCs)화불성숙DCs(imDCs)표면균표체OX62분자,imDCs저표체CD86분자,이mDCs표면고표체CD86분자.결론 건립재체외대량확증화다충감정대서골수래원DCs적방법.
Objective To identify the dendritic cells (DCs) induced and cultured by rat bone marrow stems in vitro.Methods Dendritic cells derived from rat bone marrow were induced and propagated in vitro.DCs were confirmed morphologically using H&E staining,Pap staining and Diff-Quick staining.Two surface markers for OX62 and CD86 were detected by immunohistochemistry,and immunofluorescence methods.Results DCs showed irregular shapes and nuclei with phagocytosed vesicules in the cytoplasm.Both mDCs and imDCs expressed OX62.CD86 was low expressed in imDCs,but highly expressed in mDCs.Conclusion We have established valid methods for amplification and identification of DCs.
