中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
5期
1029-1031
,共3页
韦垒%何跃明%庄冰洁%刘权焰%刘志苏%袁玉峰
韋壘%何躍明%莊冰潔%劉權燄%劉誌囌%袁玉峰
위루%하약명%장빙길%류권염%류지소%원옥봉
甲硫氨酸腺苷转移酶1A%雌激素%性别差异%肝脏
甲硫氨痠腺苷轉移酶1A%雌激素%性彆差異%肝髒
갑류안산선감전이매1A%자격소%성별차이%간장
Methionine adenosyltransferase-1A%Estradiol%Gender differences%Liver
目的 观察SD大鼠体内性激素水平的变化对大鼠肝脏组织表达甲硫氨酸腺苷转移酶1A(MAT1A)的影响.方法 选取适龄性成熟的SD大鼠,给予对应处理后构建具有性激素浓度梯度差异的SD大鼠模型(雌、雄各3组,每组6只),应用酶联免疫吸附试验(ELISA)法分别测量各组大鼠体内性激素浓度,再应用实时定量反转录聚合酶链反应(RT-qPCR)、Western blot法观察测定不同分组的大鼠的肝脏组织中MAT1A的表达.结果 ELISA法测性激素结果:空白对照雌鼠(FM0)组浓度为(43.125±11.066)ng/L,去卵巢雌鼠(FM+)组0 ng/L,去卵巢后补充雄激素的雌鼠(FM+T)组(31.738 ±7.142) ng/L(P ＜0.01);空白对照雄大鼠(M0)组浓度为(28.945±9.379) mmol/L,去势雄鼠(M+)组0 mmol/L,去势后补充雌激素的雄大鼠(M+E)组(26.158 ±7.846) mmol/L(P＜0.01).RT-qPCR结果:雌鼠MAT1A mRNA表达水平FM0组＞FM+组＞FM +T组,平均值为0.4714 ＞0.1334 ＞0.0660(P＜0.01);雄鼠MAT1A mRNA表达水平M0组＜M+组＜M+E组,平均值为0.0298 ＜0.0687 ＜0.205 5(P ＜0.01).Western blot结果:雌雄大鼠肝脏MAT1A蛋白表达趋势与RT-qPCR一致.结论 大鼠肝脏表达MAT1A存在明显性别差异,高水平的雌激素有利于MAT1A的表达,雄激素则抑制MAT1A的表达,而性激素则通过调控MAT1A的性别差异表达进而在肝癌性别差异分布中起重要调控作用.
目的 觀察SD大鼠體內性激素水平的變化對大鼠肝髒組織錶達甲硫氨痠腺苷轉移酶1A(MAT1A)的影響.方法 選取適齡性成熟的SD大鼠,給予對應處理後構建具有性激素濃度梯度差異的SD大鼠模型(雌、雄各3組,每組6隻),應用酶聯免疫吸附試驗(ELISA)法分彆測量各組大鼠體內性激素濃度,再應用實時定量反轉錄聚閤酶鏈反應(RT-qPCR)、Western blot法觀察測定不同分組的大鼠的肝髒組織中MAT1A的錶達.結果 ELISA法測性激素結果:空白對照雌鼠(FM0)組濃度為(43.125±11.066)ng/L,去卵巢雌鼠(FM+)組0 ng/L,去卵巢後補充雄激素的雌鼠(FM+T)組(31.738 ±7.142) ng/L(P ＜0.01);空白對照雄大鼠(M0)組濃度為(28.945±9.379) mmol/L,去勢雄鼠(M+)組0 mmol/L,去勢後補充雌激素的雄大鼠(M+E)組(26.158 ±7.846) mmol/L(P＜0.01).RT-qPCR結果:雌鼠MAT1A mRNA錶達水平FM0組＞FM+組＞FM +T組,平均值為0.4714 ＞0.1334 ＞0.0660(P＜0.01);雄鼠MAT1A mRNA錶達水平M0組＜M+組＜M+E組,平均值為0.0298 ＜0.0687 ＜0.205 5(P ＜0.01).Western blot結果:雌雄大鼠肝髒MAT1A蛋白錶達趨勢與RT-qPCR一緻.結論 大鼠肝髒錶達MAT1A存在明顯性彆差異,高水平的雌激素有利于MAT1A的錶達,雄激素則抑製MAT1A的錶達,而性激素則通過調控MAT1A的性彆差異錶達進而在肝癌性彆差異分佈中起重要調控作用.
목적 관찰SD대서체내성격소수평적변화대대서간장조직표체갑류안산선감전이매1A(MAT1A)적영향.방법 선취괄령성성숙적SD대서,급여대응처리후구건구유성격소농도제도차이적SD대서모형(자、웅각3조,매조6지),응용매련면역흡부시험(ELISA)법분별측량각조대서체내성격소농도,재응용실시정량반전록취합매련반응(RT-qPCR)、Western blot법관찰측정불동분조적대서적간장조직중MAT1A적표체.결과 ELISA법측성격소결과:공백대조자서(FM0)조농도위(43.125±11.066)ng/L,거란소자서(FM+)조0 ng/L,거란소후보충웅격소적자서(FM+T)조(31.738 ±7.142) ng/L(P ＜0.01);공백대조웅대서(M0)조농도위(28.945±9.379) mmol/L,거세웅서(M+)조0 mmol/L,거세후보충자격소적웅대서(M+E)조(26.158 ±7.846) mmol/L(P＜0.01).RT-qPCR결과:자서MAT1A mRNA표체수평FM0조＞FM+조＞FM +T조,평균치위0.4714 ＞0.1334 ＞0.0660(P＜0.01);웅서MAT1A mRNA표체수평M0조＜M+조＜M+E조,평균치위0.0298 ＜0.0687 ＜0.205 5(P ＜0.01).Western blot결과:자웅대서간장MAT1A단백표체추세여RT-qPCR일치.결론 대서간장표체MAT1A존재명현성별차이,고수평적자격소유리우MAT1A적표체,웅격소칙억제MAT1A적표체,이성격소칙통과조공MAT1A적성별차이표체진이재간암성별차이분포중기중요조공작용.
Objective To observe the changes of Sprague-Dawley rat sex hormone levels influence the expression of the methionine adenosyltransferase-1A (MAT1 A) in rats liver tissues.Methods To select 36 sexually mature Sprague-Dawley rats,build a SD rat model of sex hormone concentration gradient difference (male and female have three groups,each group of 6).Using enzyme linked immunosorbent assay (ELISA) method to measure the sex hormone concentrations of each group rats and the expressions of MAT1A were examined by the Western blotting and real-time quantitative polymerase chain reaction (RT-qPCR).Results ELISA' s results:Estrogen concentrations of FM0 group is (43.125 ±11.066) ng/L,FM + group is 0 ng/L,FM + T group is (31.738 ± 7.142) ng/L (P ＜ 0.01);Androgen concentrations of M0 group is (28.945 ± 9.379) mmol/L,M + group is 0 mmol/L,M + E group is (26.158 ±7.846) mmol/L (P ＜0.01).RT-qPCR:females MAT1A expression is FM0 ＞ FM + ＞ FM +T,with a mean of 0.471 4 ＞0.133 4 ＞0.066 0 (P ＜0.01),males MAT1A expression is M0 ＜ M + ＜ M + E,with an average of 0.029 8 ＜ 0.068 7 ＜ 0.205 5 (P ＜ 0.01).Western blotting:Male and female liver MAT1A protein-expression in rats showed consistent result with the outcome of RT-qPCR.Conclusion The expression of MAT1A protein in rats have obvious gender differences,the expression of high levels of estrogen is advantageous to the MAT1A,whereas androgen inhibits the expression of MAT1A,while sex hormones through regulating MAT1A sex differences in expression to play an important role in gender differences in distribution of liver cancer.
