中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
5期
1051-1053
,共3页
尿流改道%小肠黏膜
尿流改道%小腸黏膜
뇨류개도%소장점막
Urinary diversion%Small intestinal mucosa
目的 观察长期在尿液环境中大鼠小肠黏膜结构与功能的变化.方法 将22只大鼠随机分为2组,实验组12只,截取4 cm末段小肠,接受代膀胱术;对照组10只,接受假手术.术后1个月和5个月,观察代膀胱黏膜的组织结构、吸收与分泌功能的变化,体质量、血生化的变化.结果 术后5个月,代膀胱黏膜表现为增生;杯状细胞术后1个月明显增多,术后5个月明显减少;代膀胱黏膜对水、K+,NH;,表现为吸收,1h吸收量分别为:(0.39 ±0.09) ml、(5.2±0.9)μmol、(7.11±0.87) μmol;对Na+、Cl-、HCO;,表现为分泌,1h分泌量分别为:(33.2±4.6)、(8.0±3.7)、(17.7±7.0) μmol,与术后1个月的检测值比较,差异无统计学意义(P＞0.05);黏液分泌量为(0.091 ±0.079)g,与术后1个月的检测值比较,差异有统计学意义(P＜0.05);术后5个月,实验组大鼠体质量及血生化检测值,与对照组比较,差异无统计学意义(P＞0.05).结论 长期在尿液环境中的大鼠小肠黏膜,组织结构表现为增生;杯状细胞早期增多,后期明显减少;对水、电解质的 吸收或分泌不发生明显改变;黏液分泌量逐渐减少.4 cm末段小肠构建的代膀胱,对大鼠远期健康的影响不明显.
目的 觀察長期在尿液環境中大鼠小腸黏膜結構與功能的變化.方法 將22隻大鼠隨機分為2組,實驗組12隻,截取4 cm末段小腸,接受代膀胱術;對照組10隻,接受假手術.術後1箇月和5箇月,觀察代膀胱黏膜的組織結構、吸收與分泌功能的變化,體質量、血生化的變化.結果 術後5箇月,代膀胱黏膜錶現為增生;杯狀細胞術後1箇月明顯增多,術後5箇月明顯減少;代膀胱黏膜對水、K+,NH;,錶現為吸收,1h吸收量分彆為:(0.39 ±0.09) ml、(5.2±0.9)μmol、(7.11±0.87) μmol;對Na+、Cl-、HCO;,錶現為分泌,1h分泌量分彆為:(33.2±4.6)、(8.0±3.7)、(17.7±7.0) μmol,與術後1箇月的檢測值比較,差異無統計學意義(P＞0.05);黏液分泌量為(0.091 ±0.079)g,與術後1箇月的檢測值比較,差異有統計學意義(P＜0.05);術後5箇月,實驗組大鼠體質量及血生化檢測值,與對照組比較,差異無統計學意義(P＞0.05).結論 長期在尿液環境中的大鼠小腸黏膜,組織結構錶現為增生;杯狀細胞早期增多,後期明顯減少;對水、電解質的 吸收或分泌不髮生明顯改變;黏液分泌量逐漸減少.4 cm末段小腸構建的代膀胱,對大鼠遠期健康的影響不明顯.
목적 관찰장기재뇨액배경중대서소장점막결구여공능적변화.방법 장22지대서수궤분위2조,실험조12지,절취4 cm말단소장,접수대방광술;대조조10지,접수가수술.술후1개월화5개월,관찰대방광점막적조직결구、흡수여분비공능적변화,체질량、혈생화적변화.결과 술후5개월,대방광점막표현위증생;배상세포술후1개월명현증다,술후5개월명현감소;대방광점막대수、K+,NH;,표현위흡수,1h흡수량분별위:(0.39 ±0.09) ml、(5.2±0.9)μmol、(7.11±0.87) μmol;대Na+、Cl-、HCO;,표현위분비,1h분비량분별위:(33.2±4.6)、(8.0±3.7)、(17.7±7.0) μmol,여술후1개월적검측치비교,차이무통계학의의(P＞0.05);점액분비량위(0.091 ±0.079)g,여술후1개월적검측치비교,차이유통계학의의(P＜0.05);술후5개월,실험조대서체질량급혈생화검측치,여대조조비교,차이무통계학의의(P＞0.05).결론 장기재뇨액배경중적대서소장점막,조직결구표현위증생;배상세포조기증다,후기명현감소;대수、전해질적 흡수혹분비불발생명현개변;점액분비량축점감소.4 cm말단소장구건적대방광,대대서원기건강적영향불명현.
Objective To study the histological and functional changes of rat small intestinal mucosa in urinary circumstance for a long period.Methods 22 rats divided into two groups randomly,12 rats in experiment group accepted bladder substitution operation use 4cm distal small intestine and 10 rats in control group accepted sham operation.1 month and 5 month postoperation,to observe the histological changes,secretion and absorption changes of the neobladder mucosa,and body weight,blood biochemistry changes of the rats.Results 5 month postoperation,histological hyperplasia happened in neobladder mucosa.the number of goblet cells increased apparently 1 month postoperation and decreased significantly 5month postoperation.neobladder mucosa showed absorption of water,K + and NH4+,1 h absorption were (0.39 ± 0.09) ml,(5.2 ± 0.9) μmol,(7.11 ± 0.87) μmol,respectively;showed secretion of Na +,Cl-,HCO3-,1 h secretion were (33.2 ± 4.6),(8.0 ± 3.7),(17.7 ± 7.0) μmol,respectively,compared with the data obtained 1 month postoperation,the differences were not significant (P ＞ 0.05).the weight of the residual mucus in the neobladder was (0.091 ± 0.079) g,compared with the data obtained 1 month postoperation,the difference was significant (P ＜ 0.05).5 month postoperation,body weight and blood biochemistry data in the experiment group,compared with the data in the control group,the differences were not significant (P ＞ 0.05).Conclusion After the rat small intestine mucosa is in contact with urine for a long period,the mucosa shows histological hyperplasia,the number of goblet cells increases in early period,and decreases in later period in this study;significant changes not happen in the mucosa absorption or secretion function of water and electrolyte;the weight of the residual mucus in the neobladder decrease gradually;using 4 cm distal segment of rat small intestine to form bladder substitute,not significantly effect on rat later stage healthy.
