吡格列酮预处理对雨蛙肽诱导的急性胰腺炎细胞凋亡的影响
필격렬동예처리대우와태유도적급성이선염세포조망적영향
Effects of pioglitazone pre-treating on acinar cells apoptosis induced by caerulein in acute pancreatitis
  • 万方数据
    中华消化杂志 중화소화잡지
    Chinese Journal of Digestion
    2015年 4期 235-240 ,共6页

    徐萍%娄晓丽%陈诚

    서평%루효려%진성

    吡格列酮%雨蛙肽%AR42J细胞%急性胰腺炎 吡格列酮%雨蛙肽%AR42J細胞%急性胰腺炎
    필격렬동%우와태%AR42J세포%급성이선염
    Pioglitazone%Caerulein%AR42J cells%Acute pancreatitis
    目的 研究吡格列酮预处理对雨蛙肽诱导的胰腺腺泡细胞(AR42J细胞)凋亡的影响.方法 AR42J细胞分为空白对照组(常规培养)、吡格列酮组(40 μmol/L)、雨蛙肽组(1×10-8 mol/L)、吡格列酮+雨蛙肽组(40 μmol/L吡格列酮+1×10-8 mol/L雨蛙肽)、吡格列酮+GW9662+雨蛙肽组(40 μmol/L吡格列酮+5 μmol/L GW9662+1×10-8 mol/L雨蛙肽).吡格列酮、GW9662早于雨蛙肽30 min加入.在实验的3、6、12和24 h采用MTT法检测各组细胞的增殖率,采用异硫氰酸荧光素Ⅴ/碘化丙啶染色流式细胞术检测细胞凋亡率,脱氧核苷酸末端转移酶介导的脱氧尿苷三磷酸(dUTP)缺口末端标记(TUNEL)法检测细胞凋亡,检测各组半胱天冬酶(caspase)-3、半胱天冬酶-8和半胱天冬酶-9的活性,采用JC-1染色流式细胞术检测细胞的线粒体膜电位.采用单因素方差分析和LSD检验分析数据.结果 作用6、12、24 h时吡格列酮组和吡格列酮+雨蛙肽组细胞的增殖活性分别为0.19±0.02、0.22±0.02、0.36±0.02和0.20±0.04、0.23±0.02、0.38±0.02,分别明显低于空白对照组(0.25±0.04、0.28±0.03、0.46±0.02,t=-3.16、-4.61、-6.25)和雨蛙肽组(0.23±0.02、0.29±0.01、0.46±0.05,t=-1.58、-4.61、-6.15,P均＜0.05),吡格列酮+GW9662+雨蛙肽组细胞增殖活性(0.23±0.02、0.27±0.02、0.45±0.01)高于吡格列酮+雨蛙肽组(t=2.25、3.87、4.56,P均＜0.05).作用6、12、24 h后,异硫氰酸荧光素Ⅴ/碘化丙啶染色流式细胞术发现吡格列酮组和吡格列酮+雨蛙肽组细胞的凋亡率分别为(11.80±0.47)％、(9.62±2.63)％、(14.92±2.52)％和(8.78±0.47)％、(11.89±2.80)％、(14.25±2.67)％,两组间差异均无统计学意义(P均＞0.05),但这两组分别明显高于空白对照组的(5.52±0.64)％、(5.30±0.97)％、(5.47±0.88)％和雨蛙肽组的(5.98±1.21)％、(7.47±0.58)％、(8.11±1.32)％,差异均有统计学意义(t照组=9.81、4.45、10.74,t蛙肽组=4.38、7.62、6.98,P均＜0.05);吡格列酮+GW9662+雨蛙肽组细胞的凋亡率[(5.82±0.26)％、(6.05±0.83)％、(9.23±0.90)％]与雨蛙肽组无明显差异,与吡格列酮+雨蛙肽组相比明显降低(t=-4.63、-10.07、-5.70,P均＜0.05).作用12h时TUNEL染色发现,吡格列酮组的凋亡率[(3.93±0.40)％]明显高于空白对照组[(2.73±0.68)％],吡格列酮+雨蛙肽组细胞凋亡率[(8.43±1.65)％]明显高于雨蛙肽组[(2.80±0.56)％],吡格列酮+GW9662+雨蛙肽组细胞凋亡率[(3.87±0.35)％]低于吡格列酮+雨蛙肽组(t=7.93、8.92、-5.35,P均＜0.05).作用24 h时吡格列酮+雨蛙肽组细胞的半胱天冬酶-3、半胱天冬酶-8和半胱天冬酶-9活性(1.28±0.05、1.38±0.04、1.53±0.09)与雨蛙肽组(1.12±0.88、1.22±0.02、0.53±0.07)相比显著升高(t=3.20、8.62、1.29,P均＜0.05),经GW9662干预后,半胱天冬酶类的活性部分恢复.吡格列酮组、吡格列酮+雨蛙肽组线粒体膜电位改变细胞数目明显多于雨蛙肽处理组[(28.50±0.91)％、(28.20±2.56)％比(15.00±3.67)％,t=8.10、10.02,P均＜0.05],而加入GW9662可使膜电位部分恢复[(20.67±2.20)％].结论 吡格列酮可能通过激活半胱天冬酶的活性,改变细胞膜电位促进AR42J细胞的凋亡,而其拮抗剂GW9662能部分抑制吡格列酮的促凋亡功能.
    목적 연구필격렬동예처리대우와태유도적이선선포세포(AR42J세포)조망적영향.방법 AR42J세포분위공백대조조(상규배양)、필격렬동조(40 μmol/L)、우와태조(1×10-8 mol/L)、필격렬동+우와태조(40 μmol/L필격렬동+1×10-8 mol/L우와태)、필격렬동+GW9662+우와태조(40 μmol/L필격렬동+5 μmol/L GW9662+1×10-8 mol/L우와태).필격렬동、GW9662조우우와태30 min가입.재실험적3、6、12화24 h채용MTT법검측각조세포적증식솔,채용이류청산형광소Ⅴ/전화병정염색류식세포술검측세포조망솔,탈양핵감산말단전이매개도적탈양뇨감삼린산(dUTP)결구말단표기(TUNEL)법검측세포조망,검측각조반광천동매(caspase)-3、반광천동매-8화반광천동매-9적활성,채용JC-1염색류식세포술검측세포적선립체막전위.채용단인소방차분석화LSD검험분석수거.결과 작용6、12、24 h시필격렬동조화필격렬동+우와태조세포적증식활성분별위0.19±0.02、0.22±0.02、0.36±0.02화0.20±0.04、0.23±0.02、0.38±0.02,분별명현저우공백대조조(0.25±0.04、0.28±0.03、0.46±0.02,t=-3.16、-4.61、-6.25)화우와태조(0.23±0.02、0.29±0.01、0.46±0.05,t=-1.58、-4.61、-6.15,P균＜0.05),필격렬동+GW9662+우와태조세포증식활성(0.23±0.02、0.27±0.02、0.45±0.01)고우필격렬동+우와태조(t=2.25、3.87、4.56,P균＜0.05).작용6、12、24 h후,이류청산형광소Ⅴ/전화병정염색류식세포술발현필격렬동조화필격렬동+우와태조세포적조망솔분별위(11.80±0.47)％、(9.62±2.63)％、(14.92±2.52)％화(8.78±0.47)％、(11.89±2.80)％、(14.25±2.67)％,량조간차이균무통계학의의(P균＞0.05),단저량조분별명현고우공백대조조적(5.52±0.64)％、(5.30±0.97)％、(5.47±0.88)％화우와태조적(5.98±1.21)％、(7.47±0.58)％、(8.11±1.32)％,차이균유통계학의의(t조조=9.81、4.45、10.74,t와태조=4.38、7.62、6.98,P균＜0.05);필격렬동+GW9662+우와태조세포적조망솔[(5.82±0.26)％、(6.05±0.83)％、(9.23±0.90)％]여우와태조무명현차이,여필격렬동+우와태조상비명현강저(t=-4.63、-10.07、-5.70,P균＜0.05).작용12h시TUNEL염색발현,필격렬동조적조망솔[(3.93±0.40)％]명현고우공백대조조[(2.73±0.68)％],필격렬동+우와태조세포조망솔[(8.43±1.65)％]명현고우우와태조[(2.80±0.56)％],필격렬동+GW9662+우와태조세포조망솔[(3.87±0.35)％]저우필격렬동+우와태조(t=7.93、8.92、-5.35,P균＜0.05).작용24 h시필격렬동+우와태조세포적반광천동매-3、반광천동매-8화반광천동매-9활성(1.28±0.05、1.38±0.04、1.53±0.09)여우와태조(1.12±0.88、1.22±0.02、0.53±0.07)상비현저승고(t=3.20、8.62、1.29,P균＜0.05),경GW9662간예후,반광천동매류적활성부분회복.필격렬동조、필격렬동+우와태조선립체막전위개변세포수목명현다우우와태처리조[(28.50±0.91)％、(28.20±2.56)％비(15.00±3.67)％,t=8.10、10.02,P균＜0.05],이가입GW9662가사막전위부분회복[(20.67±2.20)％].결론 필격렬동가능통과격활반광천동매적활성,개변세포막전위촉진AR42J세포적조망,이기길항제GW9662능부분억제필격렬동적촉조망공능.
    Objective To investigate the effects of pioglitazone pre-treating on pancreatic acinar cell (AR42J cells) apoptosis induced by caerulein.Methods AR42J cells were divided into blank control group (with normal culture),pioglitazone group (40 μmol/L),caerulein control group (1 × 10-8 mol/L),pioglitazone+ caerulein group (40 μmol/L pioglitazone + 1 × 10-8 mol/L caerulein) and pioglitazone + GW9662+caerulein group (40 μmol/L pioglitazone+ 5 μmol/L GW9662 + 1 × 10-8 mol/L caerulein).Pioglitazone and GW9662 were added 30 minutes earlier than caerulein.Cell proliferation rate of each group was determined by MTT assay at three,six,12 and 24 hour.The cell apoptosis rate was detected by flow cytometry with Annexin Ⅴ/PI staining and terminal dexynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) staining.The activity of Caspase 3,8 and 9 of each group was measured.Mitochondrial membrane potential (MMP) was detected by flow cytometry with JC-1 staining.Single factor analysis of variance and LSD test were performed for data analysis.Results At six,12 and 24 hour,the cell proliferation rate of pioglitazone group and pioglitazone + caerulein group was 0.19±0.02,0.22±0.02,0.36±0.02 and 0.20±0.04,0.23±0.02,0.38±0.02,respectively,which were significantly lower than those of blank control group (0.25 ±0.04,0.28 ± 0.03 and 0.46±0.02) and caerulein group (0.23±0.02,0.29±0.01 and 0.46±0.05,t lgroup=-3.16,-4.61 and-6.25,tcaerulein group =-1.58,-4.61 and-6.15,all P＜0.05).And the cell proliferation rates of pioglitazone+GW9662+caerulein group at six,12 and 24 hour (0.23±0.02,0.27±0.02 and 0.45±0.01) were significantly higher than those of pioglitazone+caerulein group (t=2.25、3.87、4.56,all P＜0.05).There was no significant difference in cell apoptosis rate detected by flow cytometry with Annexin Ⅴ/PI staining between pioglitazone group ((11.80 ± 0.47) ％,(9.62 ± 2.63) ％ and (14.92 ± 2.52) ％) and pioglitazone+caerulein group ((8.78±0.47)％,(11.89±2.80)％ and (14.25±2.67)％,all P＞0.05),but cell apoptosis of these two groups were higher than those of control group ((5.52± 0.64)％,(5.30±0.97)％ and (5.47±0.88)％) and caerulein group ((5.98±1.21)％,(7.47± 0.58) ％ and (8.11 ± 1.32) ％) respectively,and the differences were statistically significant (t l group =9.81,4.45 and 10.74,tcaerulein group =4.38,7.62 and 6.98,all P ＜0.05).There was no significant difference in apoptosis rate between pioglitazone+GW9662+caerulein group ((5.82±0.26) ％,(6.05± 0.83) ％ and (9.23±0.90)％) and caerulein group; while significantly higher when compared with those of pioglitazone+ caerulein group (t=-4.63,-10.07 and-5.70,all P＜0.05).At 12 hour,the apoptosis rate detected by TUNEL staining of pioglitazone group ((3.93 ± 0.40)％) was significantly higher than that of control group ((2.73 ±0.68) ％),the apoptosis rate of pioglitazone+ caerulein group ((8.43 ± 1.65)％) was significantly higher than that of caerulein group ((2.80 ± 0.56)％),the apoptosis rate of pioglitazone+GW9662+caerulein group ((3.87±0.35)％) was lower than that of pioglitazone+ caerulein group (t=7.93,8.92,-5.35,all P＜0.05).At 24 hour,the activity of Caspase 3,8 and 9 of pioglitazone+ caerulein group (1.28 ± 0.05,1.38 ± 0.04 and 1.53 ± 0.09) significantly increased compared with those of caerulein group (1.12±0.88,1.22±0.02 and 0.53±0.07,t=3.20,8.62 and 1.29,all P＜0.05).After treated with GW9662,part of activity of Caspase enzymes recovered.The number of cells with potential change of mitochondrial membrane in pioglitazone group and pioglitazone + caerulein group was more than that of caerulein group (28.50±0.91)％ and (28.20±2.56)％ vs (15.00±3.67)％) and part of membrane potential recovered after GW9662 added ((20.67 ± 2.20) ％).Conclusions Pioglitazone might promote AR42J cell apoptosis through the activation of caspases enzymes and changing membrane potential.And the antagonist GW9662 would partially inhibit the apoptosis induced by pioglitazone.
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