中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
5期
1087-1089
,共3页
郑靖阳%林锋%瞿尔力%陈清
鄭靖暘%林鋒%瞿爾力%陳清
정정양%림봉%구이력%진청
间充质干细胞%呼吸机所致肺损伤%中性粒细胞%炎症
間充質榦細胞%呼吸機所緻肺損傷%中性粒細胞%炎癥
간충질간세포%호흡궤소치폐손상%중성립세포%염증
Mesenchymal stem cells%Ventilator-induced lung injury%Polymorphonuclear%Inflammatory
目的 探讨间充质干细胞(MSCs)对大鼠呼吸机所致肺损伤的保护作用及其机制.方法 SD大鼠30只,采用大潮气量机械通气法制备呼吸机所致肺损伤的大鼠模型,按照随机数字表法分为3组,假手术组、对照组和MSCs治疗组,每组各10只.机械通气后4h处死大鼠,分别进行大鼠肺组织病理及损伤评分、肺组织中性粒细胞浸润数量测定和肺泡灌洗液中炎性因子水平测定.结果 机械通气4h后,MSCs治疗组大鼠肺组织损伤评分为(4.23±1.64)分,显著低于对照组[(7.31 ±2.15)分,t=6.427,P＜0.01],和假手术组比较差异无统计学意义[(1.97±1.31)分,t=2.22,P＞0.05].假手术组、对照组和MSCs治疗组大鼠肺组织中性粒细胞浸润数量分别为(9.27±4.55)、(26.37 ±9.44)、(15.33 ±6.62)个.MSCs治疗组中性粒细胞浸润数量和与对照组比较差异有统计学意义(t=3.011,P＜0.05),与假手术组比较差异无统计学意义(t=1.234,P＞0.05).MSCs治疗组大鼠肺泡灌洗液中白细胞介素(IL)-1β、IL-6和肿瘤坏死因子-α(TNF-α)水平分别为(70.40 ±31.40)、(41.37±14.34)和(47.60±10.23) ng/L,显著低于对照组(170.20±58.90)、(99.30±31.97)和(114.30±24.60) ng/L,差异均有统计学意义(=5.04,P＜0.01;t=3.09,P＜0.05;t =6.33,P＜0.01),和假手术组比较差异无统计学意义[(37.90±12.70) ng/L,t=1.27,P＞0.05;(29.20 ±9.11) ng/L,t=2.33,P＞0.05;(39.44±12.14) ng/L,t =0.91,P＞0.05].结论 MSCs可以减轻大潮气量机械通气所致的肺部组织损伤、肺部中性粒细胞浸润、降低局部炎性介质的释放,对呼吸机所致肺损伤具有保护作用.
目的 探討間充質榦細胞(MSCs)對大鼠呼吸機所緻肺損傷的保護作用及其機製.方法 SD大鼠30隻,採用大潮氣量機械通氣法製備呼吸機所緻肺損傷的大鼠模型,按照隨機數字錶法分為3組,假手術組、對照組和MSCs治療組,每組各10隻.機械通氣後4h處死大鼠,分彆進行大鼠肺組織病理及損傷評分、肺組織中性粒細胞浸潤數量測定和肺泡灌洗液中炎性因子水平測定.結果 機械通氣4h後,MSCs治療組大鼠肺組織損傷評分為(4.23±1.64)分,顯著低于對照組[(7.31 ±2.15)分,t=6.427,P＜0.01],和假手術組比較差異無統計學意義[(1.97±1.31)分,t=2.22,P＞0.05].假手術組、對照組和MSCs治療組大鼠肺組織中性粒細胞浸潤數量分彆為(9.27±4.55)、(26.37 ±9.44)、(15.33 ±6.62)箇.MSCs治療組中性粒細胞浸潤數量和與對照組比較差異有統計學意義(t=3.011,P＜0.05),與假手術組比較差異無統計學意義(t=1.234,P＞0.05).MSCs治療組大鼠肺泡灌洗液中白細胞介素(IL)-1β、IL-6和腫瘤壞死因子-α(TNF-α)水平分彆為(70.40 ±31.40)、(41.37±14.34)和(47.60±10.23) ng/L,顯著低于對照組(170.20±58.90)、(99.30±31.97)和(114.30±24.60) ng/L,差異均有統計學意義(=5.04,P＜0.01;t=3.09,P＜0.05;t =6.33,P＜0.01),和假手術組比較差異無統計學意義[(37.90±12.70) ng/L,t=1.27,P＞0.05;(29.20 ±9.11) ng/L,t=2.33,P＞0.05;(39.44±12.14) ng/L,t =0.91,P＞0.05].結論 MSCs可以減輕大潮氣量機械通氣所緻的肺部組織損傷、肺部中性粒細胞浸潤、降低跼部炎性介質的釋放,對呼吸機所緻肺損傷具有保護作用.
목적 탐토간충질간세포(MSCs)대대서호흡궤소치폐손상적보호작용급기궤제.방법 SD대서30지,채용대조기량궤계통기법제비호흡궤소치폐손상적대서모형,안조수궤수자표법분위3조,가수술조、대조조화MSCs치료조,매조각10지.궤계통기후4h처사대서,분별진행대서폐조직병리급손상평분、폐조직중성립세포침윤수량측정화폐포관세액중염성인자수평측정.결과 궤계통기4h후,MSCs치료조대서폐조직손상평분위(4.23±1.64)분,현저저우대조조[(7.31 ±2.15)분,t=6.427,P＜0.01],화가수술조비교차이무통계학의의[(1.97±1.31)분,t=2.22,P＞0.05].가수술조、대조조화MSCs치료조대서폐조직중성립세포침윤수량분별위(9.27±4.55)、(26.37 ±9.44)、(15.33 ±6.62)개.MSCs치료조중성립세포침윤수량화여대조조비교차이유통계학의의(t=3.011,P＜0.05),여가수술조비교차이무통계학의의(t=1.234,P＞0.05).MSCs치료조대서폐포관세액중백세포개소(IL)-1β、IL-6화종류배사인자-α(TNF-α)수평분별위(70.40 ±31.40)、(41.37±14.34)화(47.60±10.23) ng/L,현저저우대조조(170.20±58.90)、(99.30±31.97)화(114.30±24.60) ng/L,차이균유통계학의의(=5.04,P＜0.01;t=3.09,P＜0.05;t =6.33,P＜0.01),화가수술조비교차이무통계학의의[(37.90±12.70) ng/L,t=1.27,P＞0.05;(29.20 ±9.11) ng/L,t=2.33,P＞0.05;(39.44±12.14) ng/L,t =0.91,P＞0.05].결론 MSCs가이감경대조기량궤계통기소치적폐부조직손상、폐부중성립세포침윤、강저국부염성개질적석방,대호흡궤소치폐손상구유보호작용.
Objective To investigate the protective role of mesenchymal stem cells (MSCs) in ventilator-induced lung injury (VILI) in rats and its mechanisms.Methods Rat VILI model was established by high tidal volume ventilation in SD rats and randomly divided into 3 groups:sham operated group,control group and MSCs treatment group (n =10 per groups).The lung tissue injury,lung polymorphonuclear (PMN) cells counts and pro-inflammatory cytokines [interleukin (IL)-1β,IL-6 and tumor necrosis factor-α (TNF-α)] levels in bronchoalveolar lavage fluid (BALF) were measured 4 h after mechanical ventilation.Results 4 h after mechanical ventilation,the lung tissue injury score of MSCs group was 4.23 ± 1.64,significantly lower than that of control group (7.31 ± 2.15,t =6.427,P ＜ 0.01),and similar to the sham group (1.97 ± 1.31,t =2.22,P ＞ 0.05).The lung PMN counts of three groups were 9.27 ±4.55,26.37 ±9.44 and 15.33 ±6.62,respectively.The PMN counts of MSCs group was significantly lower than that of saline group (t =3.011,P ＜ 0.05) and similar to the sham group (t =1.234,P ＞0.05).The IL-1β,IL-6 and TNF-α levels in BALF of MSCs group was (70.40 ± 31.40),(41.37 ±14.34) and (47.60 ± 10.23) ng/L,respectively,significantly lower than that of control group [(170.20 ±58.90) ng/L,t=5.04,P＜0.01;(99.30 ±31.97) ng/L,t=3.09,P＜0.05;(114.30 ±24.60) ng/L,t =6.33,P ＜ 0.01] and similar to the sham group [(37.90 ± 12.70) ng/L,t =1.27,P＞0.05;(29.20±9.11) ng/L,t=2.33,P＞0.05;(39.44±12.14) ng/L,t=0.91,P＞0.05].Conclusion MSCs has a protective effect on VILI by attenuating the degree of lung tissue injury,PMN lung infiltration and production of local pro-inflammatory cytokines.
