中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
5期
1090-1092
,共3页
王海龙%卢兆桐%袁耒%张波
王海龍%盧兆桐%袁耒%張波
왕해룡%로조동%원뢰%장파
肺爆震伤%家兔%炎性因子%西维来司钠
肺爆震傷%傢兔%炎性因子%西維來司鈉
폐폭진상%가토%염성인자%서유래사납
Blast lung injury%Rabbit%Inflammatory factor%Sivelestat sodium
目的 探讨家兔早期肺爆震伤中中性粒弹性蛋白酶(NE)的变化及西维来司钠的治疗作用.方法 将30只健康新西兰大白兔随机分为:A组(对照组)和B组治疗组(西维来司钠).治疗组伤后立即给予西维来司钠10 mg/kg耳缘静脉注射;对照组经耳缘静脉注射同等剂量的生理盐水.检测治疗前及伤后血浆中肿瘤坏死因子(TNF)-α含量及NE活性.结果 西维来司钠治疗组治疗后氧分压(PO2)在1、6、12h3个时间点分别为(89.1±9.7)、(90.9±10.5)、(101.1±7.0) mmHg(1 mmHg =0.133 kPa),较对照组的(81.3±8.7)、(74.2±7.8)、(94.2±9.8)mmHg明显升高(P＜0.05),TNF-α含量在1、6、12h3个时间点治疗组分别为(122.8 ±13.6)、(85.1±12.9)、(81.1±7.2)ng/L,较对照组的(151.4 ±12.1)、(101.3±10.8)、(93.8±10.5)ng/L明显下降(P＜0.05)、NE活性在1、6、12、24 h各时间点治疗组分别为(70.5 ±6.1)、(104.8±5.4)、(84.5±4.6)、(56.3±8.6)ng/L,较对照组的(91.8±6.3)、(160.5±9.5)、(127.7±8.3)、(109.4±7.2) ng/L均明显下降(P＜0.05).结论 NE活性在肺爆震伤病情发生、发展中起到重要作用.西维来司钠治疗肺爆震伤后早期可以明显抑制血浆NE活性及炎性细胞因子TNF-α的释放,减轻中性粒细胞浸润.
目的 探討傢兔早期肺爆震傷中中性粒彈性蛋白酶(NE)的變化及西維來司鈉的治療作用.方法 將30隻健康新西蘭大白兔隨機分為:A組(對照組)和B組治療組(西維來司鈉).治療組傷後立即給予西維來司鈉10 mg/kg耳緣靜脈註射;對照組經耳緣靜脈註射同等劑量的生理鹽水.檢測治療前及傷後血漿中腫瘤壞死因子(TNF)-α含量及NE活性.結果 西維來司鈉治療組治療後氧分壓(PO2)在1、6、12h3箇時間點分彆為(89.1±9.7)、(90.9±10.5)、(101.1±7.0) mmHg(1 mmHg =0.133 kPa),較對照組的(81.3±8.7)、(74.2±7.8)、(94.2±9.8)mmHg明顯升高(P＜0.05),TNF-α含量在1、6、12h3箇時間點治療組分彆為(122.8 ±13.6)、(85.1±12.9)、(81.1±7.2)ng/L,較對照組的(151.4 ±12.1)、(101.3±10.8)、(93.8±10.5)ng/L明顯下降(P＜0.05)、NE活性在1、6、12、24 h各時間點治療組分彆為(70.5 ±6.1)、(104.8±5.4)、(84.5±4.6)、(56.3±8.6)ng/L,較對照組的(91.8±6.3)、(160.5±9.5)、(127.7±8.3)、(109.4±7.2) ng/L均明顯下降(P＜0.05).結論 NE活性在肺爆震傷病情髮生、髮展中起到重要作用.西維來司鈉治療肺爆震傷後早期可以明顯抑製血漿NE活性及炎性細胞因子TNF-α的釋放,減輕中性粒細胞浸潤.
목적 탐토가토조기폐폭진상중중성립탄성단백매(NE)적변화급서유래사납적치료작용.방법 장30지건강신서란대백토수궤분위:A조(대조조)화B조치료조(서유래사납).치료조상후립즉급여서유래사납10 mg/kg이연정맥주사;대조조경이연정맥주사동등제량적생리염수.검측치료전급상후혈장중종류배사인자(TNF)-α함량급NE활성.결과 서유래사납치료조치료후양분압(PO2)재1、6、12h3개시간점분별위(89.1±9.7)、(90.9±10.5)、(101.1±7.0) mmHg(1 mmHg =0.133 kPa),교대조조적(81.3±8.7)、(74.2±7.8)、(94.2±9.8)mmHg명현승고(P＜0.05),TNF-α함량재1、6、12h3개시간점치료조분별위(122.8 ±13.6)、(85.1±12.9)、(81.1±7.2)ng/L,교대조조적(151.4 ±12.1)、(101.3±10.8)、(93.8±10.5)ng/L명현하강(P＜0.05)、NE활성재1、6、12、24 h각시간점치료조분별위(70.5 ±6.1)、(104.8±5.4)、(84.5±4.6)、(56.3±8.6)ng/L,교대조조적(91.8±6.3)、(160.5±9.5)、(127.7±8.3)、(109.4±7.2) ng/L균명현하강(P＜0.05).결론 NE활성재폐폭진상병정발생、발전중기도중요작용.서유래사납치료폐폭진상후조기가이명현억제혈장NE활성급염성세포인자TNF-α적석방,감경중성립세포침윤.
Objective To investigate the changes of neutrophil elastase (NE) and the therapeutic effects of sivelestat sodium on early blast lung injury in rabbits.Methods 30 healthy New Zealand rabbits were randomly divided into:group A was the control group;B group was the treatment group (sivelestat sodium).Given the sivelestat sodium 10mg/kg through ear vein injection immediately after injury to treatment group;saline control group,intravenous injection of the same dose.Tumor necrosis factor-α (TNF-α) content and NE activity in plasma were measured before treatment and after injury.Results PO2 of Sivelestat treated group were (89.1 ±9.7),(90.9 ±10.5),(101.1 ±7.0) mmHg (1 mmHg =0.133 kPa)that was significantly higher than the control group [(81.3 ±8.7),(74.2 ±7.8),(94.2 ±9.8) mmHg]at 1,6,12 three time points (P ＜0.05),TNF-α content of the treated group (122.8 ± 13.6),(85.1 ± 12.9),(81.1 ± 7.2) ng/L decreased significantly compared with the control group (151.4 ± 12.1),(101.3 ± 10.8),(93.8 ± 10.5) ng/L at 1,6,12 three time points (P ＜0.05),NE activity of the treated group (70.5 ±6.1),(104.8 ±5.4),(84.5 ±4.6),(56.3 ±8.6) ng/L decreased significantly compared with the control group (91.8 ±6.3),(160.5 ±9.5),(127.7 ±8.3),(109.4 ±7.2) ng/L at all 1,6,12,24 four time points (P ＜ 0.05).Conclusion NE activity played an important role in the occurrence and development.Sivelestat sodium can inhibit the activity of plasma NE and inflammatory cytokine release of TNF-α,reduce the infiltration of neutrophils after early blast lung injury.
