中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
5期
1104-1106
,共3页
代光成%杨金辉%王瑞涛%周焱%刘江云%薛波新
代光成%楊金輝%王瑞濤%週焱%劉江雲%薛波新
대광성%양금휘%왕서도%주염%류강운%설파신
非细菌性前列腺炎%凤尾草%总黄酮%炎性因子
非細菌性前列腺炎%鳳尾草%總黃酮%炎性因子
비세균성전렬선염%봉미초%총황동%염성인자
Chronic nonbacterial prostatitis%Pteris multifida%Total flavonoids%Inflammatory factors
目的 观察凤尾草总黄酮对慢性非细菌性前列腺炎模型大鼠的治疗作用,探讨其作用机制.方法 Wistar雄性大鼠58只,腹腔注射大鼠前列腺蛋白提纯液辅以免疫佐剂制备非细菌性前列腺炎大鼠模型,造模后随机分为空白组、模型组、凤尾草低中高剂量组、普适泰组,每组8只,灌胃给予生理盐水及相应药物,连续给药30 d,观察大鼠体质量及前列腺湿重变化及免疫器官重量变化,光镜下观察各组大鼠前列腺组织的炎性细胞浸润,并应用酶联免疫吸附试验(ELISA)法检测大鼠血清中白细胞介素(IL)-8、IL-10、肿瘤坏死因子-α(TNF-α)的水平.结果 凤尾草各组并不引起大鼠的体质量、前列腺湿重的明显变化,凤尾草高剂量组大鼠脾脏胸腺重量[(0.643 ±0.042)、(0.287 ±0.042)g]与模型组[(0.701 ±0.054)、(0.354±0.051)g]比较明显下降,差异有统计学意义(P ＜0.05);组织病理学观察提示凤尾草总黄酮能明显改善模型大鼠前列腺组织结构破坏及间质水肿,降低炎性细胞浸润水平,其中凤尾草高剂量组改善最为明显.与空白组[(4.69±0.67)、(79.95 ±2.84)、(44.73±1.53) ng/L]比较,模型组血清IL-8、IL-10、TNF-α水平[(5.31±0.26)、(88.94±3.12)、(47.34±1.91) ng/L]均有所升高,差异有统计学意义(P＜0.05);凤尾草高剂量组血清IL-8和IL-10水平[(5.07±0.42)、(82.32±3.09) ng/L]比模型组[(5.31±0.26)、(88.94±3.12) ng/L]明显降低,差异有统计学意义(P＜0.05),其血清TNF-α水平(44.73±1.53)比(47.34±1.91) ng/L差异有统计学意义(P＜0.01);凤尾草中剂量组血清IL-8和TNF-α水平[(5.06±0.30)、(44.80±2.20) ng/L]明显降低,与模型组比较差异有统计学意义(P＜0.05).结论 凤尾草总黄酮各剂量组均能改善大鼠慢性非细菌性前列腺炎组织病理学变化,其中凤尾草高剂量组改善明显,其作用机制可能与调节大鼠的免疫功能,平衡炎性因子的产生有关.
目的 觀察鳳尾草總黃酮對慢性非細菌性前列腺炎模型大鼠的治療作用,探討其作用機製.方法 Wistar雄性大鼠58隻,腹腔註射大鼠前列腺蛋白提純液輔以免疫佐劑製備非細菌性前列腺炎大鼠模型,造模後隨機分為空白組、模型組、鳳尾草低中高劑量組、普適泰組,每組8隻,灌胃給予生理鹽水及相應藥物,連續給藥30 d,觀察大鼠體質量及前列腺濕重變化及免疫器官重量變化,光鏡下觀察各組大鼠前列腺組織的炎性細胞浸潤,併應用酶聯免疫吸附試驗(ELISA)法檢測大鼠血清中白細胞介素(IL)-8、IL-10、腫瘤壞死因子-α(TNF-α)的水平.結果 鳳尾草各組併不引起大鼠的體質量、前列腺濕重的明顯變化,鳳尾草高劑量組大鼠脾髒胸腺重量[(0.643 ±0.042)、(0.287 ±0.042)g]與模型組[(0.701 ±0.054)、(0.354±0.051)g]比較明顯下降,差異有統計學意義(P ＜0.05);組織病理學觀察提示鳳尾草總黃酮能明顯改善模型大鼠前列腺組織結構破壞及間質水腫,降低炎性細胞浸潤水平,其中鳳尾草高劑量組改善最為明顯.與空白組[(4.69±0.67)、(79.95 ±2.84)、(44.73±1.53) ng/L]比較,模型組血清IL-8、IL-10、TNF-α水平[(5.31±0.26)、(88.94±3.12)、(47.34±1.91) ng/L]均有所升高,差異有統計學意義(P＜0.05);鳳尾草高劑量組血清IL-8和IL-10水平[(5.07±0.42)、(82.32±3.09) ng/L]比模型組[(5.31±0.26)、(88.94±3.12) ng/L]明顯降低,差異有統計學意義(P＜0.05),其血清TNF-α水平(44.73±1.53)比(47.34±1.91) ng/L差異有統計學意義(P＜0.01);鳳尾草中劑量組血清IL-8和TNF-α水平[(5.06±0.30)、(44.80±2.20) ng/L]明顯降低,與模型組比較差異有統計學意義(P＜0.05).結論 鳳尾草總黃酮各劑量組均能改善大鼠慢性非細菌性前列腺炎組織病理學變化,其中鳳尾草高劑量組改善明顯,其作用機製可能與調節大鼠的免疫功能,平衡炎性因子的產生有關.
목적 관찰봉미초총황동대만성비세균성전렬선염모형대서적치료작용,탐토기작용궤제.방법 Wistar웅성대서58지,복강주사대서전렬선단백제순액보이면역좌제제비비세균성전렬선염대서모형,조모후수궤분위공백조、모형조、봉미초저중고제량조、보괄태조,매조8지,관위급여생리염수급상응약물,련속급약30 d,관찰대서체질량급전렬선습중변화급면역기관중량변화,광경하관찰각조대서전렬선조직적염성세포침윤,병응용매련면역흡부시험(ELISA)법검측대서혈청중백세포개소(IL)-8、IL-10、종류배사인자-α(TNF-α)적수평.결과 봉미초각조병불인기대서적체질량、전렬선습중적명현변화,봉미초고제량조대서비장흉선중량[(0.643 ±0.042)、(0.287 ±0.042)g]여모형조[(0.701 ±0.054)、(0.354±0.051)g]비교명현하강,차이유통계학의의(P ＜0.05);조직병이학관찰제시봉미초총황동능명현개선모형대서전렬선조직결구파배급간질수종,강저염성세포침윤수평,기중봉미초고제량조개선최위명현.여공백조[(4.69±0.67)、(79.95 ±2.84)、(44.73±1.53) ng/L]비교,모형조혈청IL-8、IL-10、TNF-α수평[(5.31±0.26)、(88.94±3.12)、(47.34±1.91) ng/L]균유소승고,차이유통계학의의(P＜0.05);봉미초고제량조혈청IL-8화IL-10수평[(5.07±0.42)、(82.32±3.09) ng/L]비모형조[(5.31±0.26)、(88.94±3.12) ng/L]명현강저,차이유통계학의의(P＜0.05),기혈청TNF-α수평(44.73±1.53)비(47.34±1.91) ng/L차이유통계학의의(P＜0.01);봉미초중제량조혈청IL-8화TNF-α수평[(5.06±0.30)、(44.80±2.20) ng/L]명현강저,여모형조비교차이유통계학의의(P＜0.05).결론 봉미초총황동각제량조균능개선대서만성비세균성전렬선염조직병이학변화,기중봉미초고제량조개선명현,기작용궤제가능여조절대서적면역공능,평형염성인자적산생유관.
Objective To study the therapeutical effects of total flavonoid of pteris multifida in nonbacterial prostatitis rats and the possible mechanism.Methods Fifty-eight male Wistar rats were intraperitoneally injected with the Wistar rat prostate protein purification liquid mixture with immune adjuvant for the preparation of experimental rat model of chronic nonbacterial prostatitis.The rats were randomly divided into 6 groups (8 rats each group):the blank group,model group,total flavonoid of pteris multifida group (high,medium,and low dose groups),prostat group.The rats were fed on relevant drugs or the same volume of distilled water in each group,once daily for consecutive 30 days.The body weight,the immune organs weight and the prostate wet weight were measured,and the prostatic index (PI) was calculated.The levels of interleukin (IL)-8,IL-10 and tumor necrosis factor-α (TNF-α) were determined by enzyme linked immunosorbent assay (ELISA) assay kit.The pathological changes were observed in the prostate tissues under the light microscope.Results Total flavonoid of pteris multifida could not reduce the body weight and prostate wet weight.As compared with model group [(0.701 ±0.054),and (0.354 ±0.051) g],the spleen weight and thymus weight in high dose total flavonoid of pteris multifida treated group [(0.643 ± 0.042),and (0.287 ± 0.042) g] were significantly reduced (P ＜ 0.05).Under the light microscopy,total flavonoid of pteris multifida could obviously alleviate the pathological changes in the prostate tissue in rat models,reduce infiltration of inflammatory cells,most significantly in the high dose total flavonoid of pteris multifida treated group.As compared with blank group [(4.69 ± 0.67),(79.95 ±2.84),and (44.73 ± 1.53) ng/L],IL-8,IL-10 and TNF-α levels were remarkably increased in model group [(5.31 ± 0.26),(88.94 ± 3.12),and (47.34 ± 1.91) ng/L,P ＜ 0.05].As compared with model group,the serum levels of IL-8 and IL-10 in high dose total flavonoid of pteris multifida treated group was remarkably depressed [(5.07 ± 0.42,82.32 ± 3.09) vs.(5.31 ± 0.26,88.94 ±3.12) ng/L,P ＜0.05].As compared with model group [(47.34 ± 1.91) ng/L],the serum levels of TNF-α in high dose total flavonoid of pteris multifida treated group [(44.73 ± 1.53) ng/L] was significantly reduced (P ＜ 0.01).The levels of IL-8 and TNF-α [(5.06 ± 0.30),(44.80 ±2.20) ng/L] were reduced in the middle dose total flavonoid of pteris multifida treated group group as compared with model group (P ＜ 0.05).Conclusion The total flavonoids of pteris multifida could significantly alleviate the pathological changes of the prostate tissues in rats with chronic nonbacterial prostatitis,most significantly in the high dose total flavonoid of pteris multifida treated group,which might be related to the regulation of the immune function and balance of the production of inflammatory factors.
