CAJ | 학술논문

目的检测兔骨性关节炎的膝关节滑液中以及软骨下骨中骨桥蛋白(OPN)的表达,探讨烟酸对骨桥蛋白表达的影响.方法取新西兰大白兔采用改良Hulth法建立兔膝骨性关节炎模型,治疗组(12只)采用200 mg/(kg·d)烟酸的提取药物灌胃,对照组(12只)采取普通饮食喂养作为对照组.实验前后取关节骨组织,采用免疫组织化学法检测关节骨组织中OPN的含量.结果与对照组[(2 390.29±291.31) ng/L]比较,烟酸组OPN含量[(2 140.19±211.19) ng/L]显著降低.两组差异有统计学意义(P＜0.05).结论烟酸能够有效降低OPN含量,具有促进骨关节炎的修复的作用.
목적 검측토골성관절염적슬관절활액중이급연골하골중골교단백(OPN)적표체,탐토연산대골교단백표체적영향.방법 취신서란대백토채용개량Hulth법건립토슬골성관절염모형,치료조(12지)채용200 mg/(kg·d)연산적제취약물관위,대조조(12지)채취보통음식위양작위대조조.실험전후취관절골조직,채용면역조직화학법검측관절골조직중OPN적함량.결과 여대조조[(2 390.29±291.31) ng/L]비교,연산조OPN함량[(2 140.19±211.19) ng/L]현저강저.량조차이유통계학의의(P＜0.05).결론 연산능구유효강저OPN함량,구유촉진골관절염적수복적작용.
Objective To investigate effect of nicotinic acid on expression of osteopontin in rabbit of knee osteoarthritis by detect the expression of osteopontin (OPN) in the rabbit' s synovial fluid and endochondral bone.Methods New Zealand Rabbits were made into knee osteoarthritis by improvement Hulth,12 of treatment group were supplemented with niacin by using stomach tube [200 mg/(kg·d)],12of the control group were supplemented with ordinary diet.Endochondral bone were taken out before and after the experiment,and detect the content of OPN by immunocytochemistry.Results Compared with the control group [(2 390.29 ± 291.31) ng/L],the expression of OPN of niacin group [(2 140.19 ± 211.19) ng/L] decrease obviously,there were statistical differences between the control group and the niacin group (P ＜ 0.05).Conclusion Nicotinic acid can helps repair osteoarthritis by effectively lowered the content of OPN.