CAJ | 학술논문

目的探讨糖尿病大鼠盂肱关节血管内皮生长因子(VEGF)表达和血管生成的关系.方法选取体质量为240～260 g之间的无特定病原体(SPF)级雄性SD大鼠15只,随机分为两组,即模型组(10只)和对照组(5只).模型组造模成功后,将其与对照组一起处死,取肩袖间隙的滑膜组织,对其进行染色和评估.VEGF的表达通过免疫组织化学和Western blot测定.血管密度通过滑膜组织的CD34免疫反应测定.结果模型组肩袖间隙均有滑膜组织充血,其中VEGF免疫组织化学染色结果为:++++(4只),+++(4只),++(2只),较对照组[++(4只),+(1只)]免疫反应明显增强(P＜0.05).CD34免疫组织化学染色结果表明模型组和对照组每高倍视野平均血管数分别为(34.1 ±15.9)和(9.4±4.5)个(P＜0.05).Westem blot检测结果显示,目的蛋白条带明显增强.结论 VEGF在糖尿病大鼠肩袖间隙的滑膜组织中表达较多,其可能在糖尿病大鼠冻结肩的病理机制和血管生成方面起一定作用.
목적 탐토당뇨병대서우굉관절혈관내피생장인자(VEGF)표체화혈관생성적관계.방법 선취체질량위240～260 g지간적무특정병원체(SPF)급웅성SD대서15지,수궤분위량조,즉모형조(10지)화대조조(5지).모형조조모성공후,장기여대조조일기처사,취견수간극적활막조직,대기진행염색화평고.VEGF적표체통과면역조직화학화Western blot측정.혈관밀도통과활막조직적CD34면역반응측정.결과 모형조견수간극균유활막조직충혈,기중VEGF면역조직화학염색결과위:++++(4지),+++(4지),++(2지),교대조조[++(4지),+(1지)]면역반응명현증강(P＜0.05).CD34면역조직화학염색결과표명모형조화대조조매고배시야평균혈관수분별위(34.1 ±15.9)화(9.4±4.5)개(P＜0.05).Westem blot검측결과현시,목적단백조대명현증강.결론 VEGF재당뇨병대서견수간극적활막조직중표체교다,기가능재당뇨병대서동결견적병리궤제화혈관생성방면기일정작용.
Objective To explore the relationship between the expression of vascular endothelial growth factor (VEGF) and angiogenesis in glenohumeral of diabetic rats.Methods The 15 specific pathogen free (SPF) male SD rats that weighing between 240 and 260 gram were randomly divided into two groups,namely the model group (10) and the control group (5).After the success of modeling,the rates of both groups were sacrificed,take synovial tissues of rotator cuff from each group,stain and evaluate them.The expression of VEGF was determined by immunohistochemistry and Western blotting.The evalution of vascular density was measured by CD34 immunoreactivity.Results The hyperemia of synovial tissue from rotator cuff was observerd in model group,which showed strong immunoreactivity compared with the synovial tissue in control group,the immunohistochemistry of VEGF was ++++ (4),+++ (4),++ (2) compared with the control group ++ (4),+ (1) (P ＜ 0.05),the CD34 immunohistochemical staining showed that the average number of vessels per × 400 field of vision in the model group and the control group were (34.1 ± 15.9) and (9.4 ±4.5) respectively (P ＜0.05).It also shows that the expression of VEGF was more than the control group by Western blot.Conclusion VEGF is more expressed in the synovial tissue of rotator cuff in diabetic rats,which may play a role in pathological mechanisms and angiogenesis of diabetic rats frozen shoulder.