江苏预防医学
江囌預防醫學
강소예방의학
JIANGSU JOURNAL OF PREVENTIVE MEDICINE
2015年
3期
1-4
,共4页
刘明星%纪雅慧%韦晔%刘玉萍%苏志刚%黄欢欢%李建祥
劉明星%紀雅慧%韋曄%劉玉萍%囌誌剛%黃歡歡%李建祥
류명성%기아혜%위엽%류옥평%소지강%황환환%리건상
BEAS-2B 细胞%氡%恶性转化%细胞周期%凋亡率%线粒体膜电位
BEAS-2B 細胞%氡%噁性轉化%細胞週期%凋亡率%線粒體膜電位
BEAS-2B 세포%동%악성전화%세포주기%조망솔%선립체막전위
BEAS-2B cells%Radon%Malignant transformation%Cell cycle%Apoptosis rate%Mitochondrial membrane potential
目的：了解氡致 BEAS-2B 细胞早期恶性转化的特征。方法通过细胞气体染毒装置，将永生化的人支气管上皮细胞（BEAS-2B）暴露于放射性气体氡，通过测定经氡照射后细胞周期、凋亡率、线粒体膜电位，判断照射后 BEAS-2B 细胞是否存在早期恶性转化趋势。结果染氡后细胞 G1期缩短，S 期延长，染氡细胞传代后晚期代数细胞凋亡率相比早期代数细胞降低，线粒体膜电位降低。结论短期染氡后，细胞周期等指标发生变化，出现恶性转化趋势。
目的：瞭解氡緻 BEAS-2B 細胞早期噁性轉化的特徵。方法通過細胞氣體染毒裝置，將永生化的人支氣管上皮細胞（BEAS-2B）暴露于放射性氣體氡，通過測定經氡照射後細胞週期、凋亡率、線粒體膜電位，判斷照射後 BEAS-2B 細胞是否存在早期噁性轉化趨勢。結果染氡後細胞 G1期縮短，S 期延長，染氡細胞傳代後晚期代數細胞凋亡率相比早期代數細胞降低，線粒體膜電位降低。結論短期染氡後，細胞週期等指標髮生變化，齣現噁性轉化趨勢。
목적：료해동치 BEAS-2B 세포조기악성전화적특정。방법통과세포기체염독장치，장영생화적인지기관상피세포（BEAS-2B）폭로우방사성기체동，통과측정경동조사후세포주기、조망솔、선립체막전위，판단조사후 BEAS-2B 세포시부존재조기악성전화추세。결과염동후세포 G1기축단，S 기연장，염동세포전대후만기대수세포조망솔상비조기대수세포강저，선립체막전위강저。결론단기염동후，세포주기등지표발생변화，출현악성전화추세。
Objective To study early stage malignant transformation characteristics of BEAS-2B cell upon radon exposure. Methods Immortalized human bronchial epithelial cell line (BEAS-2B)was exposed to radioactive gas radon by cell gas expo-sure device.Early stage malignant transformation trend of BEAS-2B cells was evaluated based on analysis of cell cycle,apopto-sis rate and mitochondrial membrane potential.Results Upon exposure of radon,G1 stage of BEAS-2B cells shortened,while S stage extended;apoptosis rate of later stage cells was lower than that of early stage cells after cell passage;mitochondrial membrane potential decreased.Conclusion Upon short exposure to radon,series changes were observed on indices such as cell cycle and etc,demonstrating malignant transformation trend.
