中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
5期
1137-1139
,共3页
吴成稳%周志强%高建伟%马速佳%李祥波%王耀磊
吳成穩%週誌彊%高建偉%馬速佳%李祥波%王耀磊
오성은%주지강%고건위%마속가%리상파%왕요뢰
动脉粥样硬化斑块%基质金属蛋白酶-9%组织蛋白酶S
動脈粥樣硬化斑塊%基質金屬蛋白酶-9%組織蛋白酶S
동맥죽양경화반괴%기질금속단백매-9%조직단백매S
Antherosclerosis plaque%Matrix metalloproteinase-9%Cathepsin S
目的 探讨基质金属蛋白酶-9(MMP-9)及组织蛋白酶S(CatS)在动脉粥样硬化斑块中的表达.方法 收集人股动脉的粥样硬化斑块组织45例,正常脾动脉组织24例,分为稳定斑块组(20例)、不稳定斑块组(25例)以及对照组(24例),采用免疫组织化学及反转录-聚合酶链反应(RT-PCR)检测各组标本中MMP-9和CatS及其mRNA的表达,以及两者间的关系.结果 MMP-9、CatS蛋白在平滑肌细胞、泡沫细胞、单核-巨噬细胞内有较多的表达,两者及其mRNA在各组中的表达差异有统计学意义(FMMP-9=168.448,P＜0.01;FMMP-9 mRNA=221.300,P＜0.01;FCats =273.246,P＜0.01;FCats mRNA=51.681,P＜ 0.01).MMP-9、CatS蛋白及其mRNA在不稳定组(MMP-9蛋白:2.170±0.048,MMP-9 mRNA:1.588±0.085,CatS蛋白:2.231 ±0.050,CatS mRNA:1.411±1.104,P＜0.05)、稳定组(MMP-9蛋白:2.080±0.051,MMP-9 mRNA:1.346±0.071,CatS蛋白:2.097±0.045,CatS mRNA:1.280±0.058,P＜0.05)与对照组(MMP-9蛋白:1.959±0.011,MMP-9 mRNA:1.157 ±0.057,CatS蛋白:1.969 ±0.013,CatS mRNA:1.167±0.080,P＜0.05)中的表达依次降低,两者及其mRNA在稳定组、不稳定组中的表达均呈正相关(r稳定蛋白=0.690,P＜0.05;r稳定mRNA=0.739,P＜0.01;r不稳定蛋白=0.940,P＜0.01;r不稳定mRNA=0.820,P＜0.01).结论 MMP-9、CatS蛋白的表达上调可能与动脉粥样硬化的发生及斑块的不稳定性有关.
目的 探討基質金屬蛋白酶-9(MMP-9)及組織蛋白酶S(CatS)在動脈粥樣硬化斑塊中的錶達.方法 收集人股動脈的粥樣硬化斑塊組織45例,正常脾動脈組織24例,分為穩定斑塊組(20例)、不穩定斑塊組(25例)以及對照組(24例),採用免疫組織化學及反轉錄-聚閤酶鏈反應(RT-PCR)檢測各組標本中MMP-9和CatS及其mRNA的錶達,以及兩者間的關繫.結果 MMP-9、CatS蛋白在平滑肌細胞、泡沫細胞、單覈-巨噬細胞內有較多的錶達,兩者及其mRNA在各組中的錶達差異有統計學意義(FMMP-9=168.448,P＜0.01;FMMP-9 mRNA=221.300,P＜0.01;FCats =273.246,P＜0.01;FCats mRNA=51.681,P＜ 0.01).MMP-9、CatS蛋白及其mRNA在不穩定組(MMP-9蛋白:2.170±0.048,MMP-9 mRNA:1.588±0.085,CatS蛋白:2.231 ±0.050,CatS mRNA:1.411±1.104,P＜0.05)、穩定組(MMP-9蛋白:2.080±0.051,MMP-9 mRNA:1.346±0.071,CatS蛋白:2.097±0.045,CatS mRNA:1.280±0.058,P＜0.05)與對照組(MMP-9蛋白:1.959±0.011,MMP-9 mRNA:1.157 ±0.057,CatS蛋白:1.969 ±0.013,CatS mRNA:1.167±0.080,P＜0.05)中的錶達依次降低,兩者及其mRNA在穩定組、不穩定組中的錶達均呈正相關(r穩定蛋白=0.690,P＜0.05;r穩定mRNA=0.739,P＜0.01;r不穩定蛋白=0.940,P＜0.01;r不穩定mRNA=0.820,P＜0.01).結論 MMP-9、CatS蛋白的錶達上調可能與動脈粥樣硬化的髮生及斑塊的不穩定性有關.
목적 탐토기질금속단백매-9(MMP-9)급조직단백매S(CatS)재동맥죽양경화반괴중적표체.방법 수집인고동맥적죽양경화반괴조직45례,정상비동맥조직24례,분위은정반괴조(20례)、불은정반괴조(25례)이급대조조(24례),채용면역조직화학급반전록-취합매련반응(RT-PCR)검측각조표본중MMP-9화CatS급기mRNA적표체,이급량자간적관계.결과 MMP-9、CatS단백재평활기세포、포말세포、단핵-거서세포내유교다적표체,량자급기mRNA재각조중적표체차이유통계학의의(FMMP-9=168.448,P＜0.01;FMMP-9 mRNA=221.300,P＜0.01;FCats =273.246,P＜0.01;FCats mRNA=51.681,P＜ 0.01).MMP-9、CatS단백급기mRNA재불은정조(MMP-9단백:2.170±0.048,MMP-9 mRNA:1.588±0.085,CatS단백:2.231 ±0.050,CatS mRNA:1.411±1.104,P＜0.05)、은정조(MMP-9단백:2.080±0.051,MMP-9 mRNA:1.346±0.071,CatS단백:2.097±0.045,CatS mRNA:1.280±0.058,P＜0.05)여대조조(MMP-9단백:1.959±0.011,MMP-9 mRNA:1.157 ±0.057,CatS단백:1.969 ±0.013,CatS mRNA:1.167±0.080,P＜0.05)중적표체의차강저,량자급기mRNA재은정조、불은정조중적표체균정정상관(r은정단백=0.690,P＜0.05;r은정mRNA=0.739,P＜0.01;r불은정단백=0.940,P＜0.01;r불은정mRNA=0.820,P＜0.01).결론 MMP-9、CatS단백적표체상조가능여동맥죽양경화적발생급반괴적불은정성유관.
0bjective To explore the expression of matrix metalloproteinase-9 (MMP-9) and cathepsin S (CatS) in antherosclerotic plaques.Methods Forty-five cases of human femoral arterial atherosclerotic plaques and 24 cases of nomal human splenic arterial samples (control group) were collected.The atherosclerotic plaques were divided into stable group (n =20) and unstable group (n =25).The expression levels of MMP-9 and CatS were detected by immunohistochemical staining and reverse transcriptase-polymerase chain reaction (RT-PCR).The correlations between MMP-9 and CatS were analyzed in stable and unstable groups.Results MMP-9 and CatS proteins were largely expressed in smooth muscle cells,foam cells and monocyte-macrophages.There was significant difference in the expression of MMP-9 and CatS protein and mRNA among groups (FMMP-9 =168.448,P＜ 0.01;FMMP-9mRNA =221.300,P ＜0.01;FCatS =273.246,P ＜0.01;FCatS mRNA =51.681;P ＜0.01).The expression of MMP-9 and CatS protein and mRNA was reduced in unstable group (MMP-9 protein:2.170 ± 0.048,MMP-9mRNA:1.588 ±0.085,CatS protein:2.231 ±0.050,CatS mRNA:1.411 ± 1.104,P ＜0.05),followed by stable group (MMP-9 protein:2.080 ± 0.051,MMP-9 mRNA:1.346 ± 0.071,CatS protein:2.097 ±0.045,CatS mRNA:1.280 ±0.058,P ＜0.05),and control group (MMP-9 protein:1.959 ±0.011,MMP-9 mRNA:1.157 ±0.057,CatS protein:1.969 ±0.013,CatS mRNA:1.167 ±0.080,P ＜ 0.05).There was a positive correlation between the expression of MMP-9 and CatS protein and mRNA in stable group and unstable group (rstable protein =0.690,P ＜ 0.05;rstable mRNA =0.739,P ＜ 0.01;runstableprotein=0.940,P＜0.01;rustablemRNA =0.820,P ＜0.01).Conclusion The enhanced expression of MMP-9 and CatS protein was possible associated with the formation of atherosclerosis and the instability of plaques.
