中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
5期
1140-1143
,共4页
吴文艺%张丽婷%傅德强%黄种心%王朝阳%邱建龙
吳文藝%張麗婷%傅德彊%黃種心%王朝暘%邱建龍
오문예%장려정%부덕강%황충심%왕조양%구건룡
肿瘤高甲基化基因1%甲状腺乳头状癌%甲基化
腫瘤高甲基化基因1%甲狀腺乳頭狀癌%甲基化
종류고갑기화기인1%갑상선유두상암%갑기화
Hypermethylated in cancer 1%Papillary thyroid carcinoma%Methylation
目的 探讨肿瘤高甲基化基因1(HIC1)异常表达及其基因启动子区异常甲基化与甲状腺乳头状癌发生、发展及转移的关系.方法 利用实时荧光定量聚合酶链反应(FQ-PCR)法、Western blot法、亚硫酸氢盐测序(BSP)法检测40例甲状腺乳头状癌组织及相应的40例癌旁组织中HIC1的mRNA、蛋白表达水平差异及HIC1基因启动子区甲基化并进行统计学分析.结果 HIC1mRNA及蛋白表达在甲状腺乳头状癌组织中的表达量(分别为0.47±0.07、0.417±0.071)显著低于癌旁甲状腺组织(2.12±0.10、0.936±0.106,P＜0.01).HIC1 mRNA及蛋白下调与肿瘤的淋巴结转移状况(淋巴结阳性:0.21±0.02、0.191±0.031;淋巴结阴性:0.68±0.09、0.601±0.037)、年龄(＜45岁组:0.64±0.07、0.535±0.097,≥45岁组:0.19 ±0.04、0.221±0.007)及TNM分期(Ⅰ～Ⅱ期:0.58±0.06、0.494±0.051,Ⅲ～Ⅳ期:0.21±0.02、0.237±0.023)呈负相关(P＜0.05),且甲状腺乳头状癌组织中HIC1基因启动子区甲基化程度显著增高(癌组织为61.7％,正常组织为42.5％,P＜0.01).结论 HIC1低表达是甲状腺乳头状癌的发生、转移的重要原因之一,其低表达与基因启动子区过度甲基化有关.
目的 探討腫瘤高甲基化基因1(HIC1)異常錶達及其基因啟動子區異常甲基化與甲狀腺乳頭狀癌髮生、髮展及轉移的關繫.方法 利用實時熒光定量聚閤酶鏈反應(FQ-PCR)法、Western blot法、亞硫痠氫鹽測序(BSP)法檢測40例甲狀腺乳頭狀癌組織及相應的40例癌徬組織中HIC1的mRNA、蛋白錶達水平差異及HIC1基因啟動子區甲基化併進行統計學分析.結果 HIC1mRNA及蛋白錶達在甲狀腺乳頭狀癌組織中的錶達量(分彆為0.47±0.07、0.417±0.071)顯著低于癌徬甲狀腺組織(2.12±0.10、0.936±0.106,P＜0.01).HIC1 mRNA及蛋白下調與腫瘤的淋巴結轉移狀況(淋巴結暘性:0.21±0.02、0.191±0.031;淋巴結陰性:0.68±0.09、0.601±0.037)、年齡(＜45歲組:0.64±0.07、0.535±0.097,≥45歲組:0.19 ±0.04、0.221±0.007)及TNM分期(Ⅰ～Ⅱ期:0.58±0.06、0.494±0.051,Ⅲ～Ⅳ期:0.21±0.02、0.237±0.023)呈負相關(P＜0.05),且甲狀腺乳頭狀癌組織中HIC1基因啟動子區甲基化程度顯著增高(癌組織為61.7％,正常組織為42.5％,P＜0.01).結論 HIC1低錶達是甲狀腺乳頭狀癌的髮生、轉移的重要原因之一,其低錶達與基因啟動子區過度甲基化有關.
목적 탐토종류고갑기화기인1(HIC1)이상표체급기기인계동자구이상갑기화여갑상선유두상암발생、발전급전이적관계.방법 이용실시형광정량취합매련반응(FQ-PCR)법、Western blot법、아류산경염측서(BSP)법검측40례갑상선유두상암조직급상응적40례암방조직중HIC1적mRNA、단백표체수평차이급HIC1기인계동자구갑기화병진행통계학분석.결과 HIC1mRNA급단백표체재갑상선유두상암조직중적표체량(분별위0.47±0.07、0.417±0.071)현저저우암방갑상선조직(2.12±0.10、0.936±0.106,P＜0.01).HIC1 mRNA급단백하조여종류적림파결전이상황(림파결양성:0.21±0.02、0.191±0.031;림파결음성:0.68±0.09、0.601±0.037)、년령(＜45세조:0.64±0.07、0.535±0.097,≥45세조:0.19 ±0.04、0.221±0.007)급TNM분기(Ⅰ～Ⅱ기:0.58±0.06、0.494±0.051,Ⅲ～Ⅳ기:0.21±0.02、0.237±0.023)정부상관(P＜0.05),차갑상선유두상암조직중HIC1기인계동자구갑기화정도현저증고(암조직위61.7％,정상조직위42.5％,P＜0.01).결론 HIC1저표체시갑상선유두상암적발생、전이적중요원인지일,기저표체여기인계동자구과도갑기화유관.
Objective To investigate the expression and promoter methylation of hypermethylated in cancer 1 (HIC1) gene in papillary thyroid carcinoma and adjacent normal thyroid tissue,and its biological significance.Methods Bisulfite sequencing PCR (BSP),real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) and Western blotting were used to analyze the promoter methylation and expression differences of HIC1 gene in tissues of papillary thyroid carcinoma (40 cases) and adjacent normal thyroid (40 cases).Results Compared with adjacent normal thyroid tissue (2.12 ± 0.104,and 0.936 ±0.106),the expression of HIC1 mRNA and protein in papillary thyroid carcinoma was significantly decreased (0.47 ± 0.07,and 0.417 ± 0.071,P ＜ 0.01).The expression of HIC1 mRNA and protein was lower in the group with lymph node metastasis (0.21 ±0.02,and 0.191 ±0.031) than in the group without lymph node metastasis (0.68 ±0.09,and 0.601 ±0.037,P ＜0.05).Further clinical analysis demonstrated significant association of the expression of HIC1 mRNA and protein with:age of patients (＜ 45years old:0.64 ±0.07,and 0.535 ±0.097;≥45 years old:0.19 ±0.04,and 0.221 ±0.007),stage of disease (Ⅰ-Ⅱ stage:0.58 ±0.06,and 0.494 ±0.051;Ⅲ-Ⅳ stage:0.21 ±0.02,and 0.237 ±0.023,P ＜ 0.05).The rate of HIC1 gene promoter methylation was significantly higher (61.7％) than that in adjacent normal tissue (42.5％,P ＜ 0.01).Conclusion Promoter hypermethylation of HIC1 in papillary thyroid carcinoma causes decreased expression of HIC1,which may be one of major reasons for the metastasis in papillary thyroid carcinoma.
