中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
5期
1153-1155
,共3页
肝脏移植%急性排斥反应%可溶性白细胞介素-2受体%白细胞介素-6
肝髒移植%急性排斥反應%可溶性白細胞介素-2受體%白細胞介素-6
간장이식%급성배척반응%가용성백세포개소-2수체%백세포개소-6
Liver transplantation%Acute rejection%Soluble interleukin-2 receptor%Interleukin-6
目的 检测肝移植术后血清可溶性白细胞介素-2受体(sIL-2R)和胆汁白细胞介素-6(IL-6)的表达水平,探讨肝移植术后监测sIL-2R和IL-6的临床意义.方法 选取我院采用肝移植治疗的69例终末期肝病患者作为研究对象,根据术后是否发生急性排斥反应分为2组,即急性排斥反应组共36例和无排斥反应组共33例,应用酶联免疫吸附试验(ELISA)法检测患者的sIL-2R和IL-6水平,比较两组患者的表达差异.结果 移植术后第1d两组血清sIL-2R及胆汁IL-6表达水平差异无统计学意义(P＞0.05);观察组排斥反应发作前48 h血清sIL-2R为(471.82 ±66.46) pmol/L,胆汁IL-6为(621.81±76.92) pmol/L,确诊时为血清sIL-2R为(721.96±68.53) pmol/L,胆汁IL-6为(858.71 ±89.52) pmol/L,显著高于对照组(P＜0.05).经过激素冲击治疗后,血清sIL-2R及胆汁IL-6表达回落,与对照组差异无统计学意义(P＞0.05).结论 sIL-2R及胆汁IL-6与肝移植术后急性排斥反应关系密切,有望成为诊断急性排斥反应及评价疗效的敏感性、特异性指标.
目的 檢測肝移植術後血清可溶性白細胞介素-2受體(sIL-2R)和膽汁白細胞介素-6(IL-6)的錶達水平,探討肝移植術後鑑測sIL-2R和IL-6的臨床意義.方法 選取我院採用肝移植治療的69例終末期肝病患者作為研究對象,根據術後是否髮生急性排斥反應分為2組,即急性排斥反應組共36例和無排斥反應組共33例,應用酶聯免疫吸附試驗(ELISA)法檢測患者的sIL-2R和IL-6水平,比較兩組患者的錶達差異.結果 移植術後第1d兩組血清sIL-2R及膽汁IL-6錶達水平差異無統計學意義(P＞0.05);觀察組排斥反應髮作前48 h血清sIL-2R為(471.82 ±66.46) pmol/L,膽汁IL-6為(621.81±76.92) pmol/L,確診時為血清sIL-2R為(721.96±68.53) pmol/L,膽汁IL-6為(858.71 ±89.52) pmol/L,顯著高于對照組(P＜0.05).經過激素遲擊治療後,血清sIL-2R及膽汁IL-6錶達迴落,與對照組差異無統計學意義(P＞0.05).結論 sIL-2R及膽汁IL-6與肝移植術後急性排斥反應關繫密切,有望成為診斷急性排斥反應及評價療效的敏感性、特異性指標.
목적 검측간이식술후혈청가용성백세포개소-2수체(sIL-2R)화담즙백세포개소-6(IL-6)적표체수평,탐토간이식술후감측sIL-2R화IL-6적림상의의.방법 선취아원채용간이식치료적69례종말기간병환자작위연구대상,근거술후시부발생급성배척반응분위2조,즉급성배척반응조공36례화무배척반응조공33례,응용매련면역흡부시험(ELISA)법검측환자적sIL-2R화IL-6수평,비교량조환자적표체차이.결과 이식술후제1d량조혈청sIL-2R급담즙IL-6표체수평차이무통계학의의(P＞0.05);관찰조배척반응발작전48 h혈청sIL-2R위(471.82 ±66.46) pmol/L,담즙IL-6위(621.81±76.92) pmol/L,학진시위혈청sIL-2R위(721.96±68.53) pmol/L,담즙IL-6위(858.71 ±89.52) pmol/L,현저고우대조조(P＜0.05).경과격소충격치료후,혈청sIL-2R급담즙IL-6표체회락,여대조조차이무통계학의의(P＞0.05).결론 sIL-2R급담즙IL-6여간이식술후급성배척반응관계밀절,유망성위진단급성배척반응급평개료효적민감성、특이성지표.
Objective To inspect the expression of serum soluble interleukin-2 receptor (sIL-2R) and interleukin-6 (IL-6) in bile after liver transplantation and to discuss clinical inspection significance of sIL-2R and IL-6 after liver transplantation.Methods From December 2012 to February 2014,we selected 69 patients with end-stage liver disease as research subjects after liver transplantation in our hospital.They were divided into 2 groups according to postoperative acute rejection,the patients with postoperative acute rejection group,n =36,none acute rejection group,n =33.The sIL-2R and IL-6 levels were detected by using sandwich enzyme-linked immunosorbent assay,the expression differences were compared between the two groups.Results There were no significant differences in the expressions of bile IL-6 and sIL-2R on the first day after transplantation (P ＞ 0.05);Serum sIL-2R was (471.82 ± 66.46) pmol/L and bile IL-6 was (621.81 ±76.92) pmol/L 48 hours before acute rejection in observation group.When they were diagnosed,the serum sIL-2R was (721.96 ±68.53) pmol/L and bile IL-6 was (858.71 ±89.52) pmol/L,they were significantly higher than the control group (P ＜0.05).After steroid pulse therapy,the expressions of serum sIL-2R and bile IL-6 were decrease,there were no significant differences when compared with the control group (P ＞ 0.05).Conclusion There was close relationship between SIL-2R,bile IL-6 and acute rejection after liver transplantation.It was expected to be the sensitivity and specificity indexes in the diagnosis of acute rejection and evaluation of curative effect.
