CAJ | 학술논문

目的观察叉状头/翅膀状螺旋转录因子3(Foxp3)阳性调节性T细胞(Treg)在肝外胆管癌(EHCC)组织中的浸润,探讨环氧合酶-2(COX-2)蛋白表达与Foxp3+ Treg细胞浸润的关系.方法采用免疫组织化学法检测Foxp3+ Treg在94例胆管癌组织和COX-2蛋白在77例胆管癌组织中的表达,分析浸润细胞以及COX-2蛋白表达与临床和预后的关系,观察肝外胆管癌组织中COX-2蛋白表达对Foxp3+ Treg浸润的影响.结果 94例肝外胆管癌组织中均存在浸润的Foxp3+ Treg细胞,中位值为2.35个/高倍视野.Foxp3+ Treg细胞的浸润与肝外胆管癌患者的组织学分级、临床分期和淋巴结转移无明显相关(P＞0.05).但Foxp3+ Treg细胞低计数组患者的生存时间明显高于高计数组(P＜0.05).多因素分析表明Foxp3+Treg细胞浸润是肝外胆管癌预后的独立影响因素.77例胆管癌组织中COX-2表达阳性率为81.8％.COX-2蛋白的表达与组织学分级、临床分期和淋巴结转移无明显相关(P＞0.05).COX-2蛋白表达与Foxp3+ Treg细胞浸润之间呈正相关(r=4.89,P ＜0.05).结论肝外胆管癌组织中Foxp3+ Treg细胞浸润与肝外胆管癌患者的预后负相关,是影响肝外胆管癌预后的独立影响因素.COX-2蛋白表达可能促进Foxp3+ Treg细胞的浸润.
목적 관찰차상두/시방상라선전록인자3(Foxp3)양성조절성T세포(Treg)재간외담관암(EHCC)조직중적침윤,탐토배양합매-2(COX-2)단백표체여Foxp3+ Treg세포침윤적관계.방법 채용면역조직화학법검측Foxp3+ Treg재94례담관암조직화COX-2단백재77례담관암조직중적표체,분석침윤세포이급COX-2단백표체여림상화예후적관계,관찰간외담관암조직중COX-2단백표체대Foxp3+ Treg침윤적영향.결과 94례간외담관암조직중균존재침윤적Foxp3+ Treg세포,중위치위2.35개/고배시야.Foxp3+ Treg세포적침윤여간외담관암환자적조직학분급、림상분기화림파결전이무명현상관(P＞0.05).단Foxp3+ Treg세포저계수조환자적생존시간명현고우고계수조(P＜0.05).다인소분석표명Foxp3+Treg세포침윤시간외담관암예후적독립영향인소.77례담관암조직중COX-2표체양성솔위81.8％.COX-2단백적표체여조직학분급、림상분기화림파결전이무명현상관(P＞0.05).COX-2단백표체여Foxp3+ Treg세포침윤지간정정상관(r=4.89,P ＜0.05).결론 간외담관암조직중Foxp3+ Treg세포침윤여간외담관암환자적예후부상관,시영향간외담관암예후적독립영향인소.COX-2단백표체가능촉진Foxp3+ Treg세포적침윤.
Objective To explore the infiltration of regulatory T cells (Tregs) and its significance in extrahepatic cholangiocarcinoma (EHCC) and to elucidate the putative effects of cytochrome C oxidase-2 (COX-2) expression on the infiltration of Tregs.Methods The infiltration of forkhead/winged helix transcription factor P3 (Foxp3) + Tregs and the expression of COX-2 were studied by immunohistochemistry in 94 and 77 cases of EHCC respectively.The correlation between the expression of COX-2 and Foxp3 +Tregs infiltration with clinicopathological features and prognosis was analyzed.The influence of COX-2 expression on infihration of Foxp3 + Tregs was evaluated.Results Foxp3 + Tregs infiltration was seen in all the 94 cases of EHCC with the median Foxp3 + Tregs number being 2.35 per high-power field (HPF).There was no significant correlation between the number of infiltrated Foxp3 + Tregs in cancer tissue and histological grading,TNM stages and lymph node metastasis of EHCC.The patients with less number of infiltrated Foxp3 + Tregs showed significantly longer survival than those with greater number of infiltrated Foxp3 + Tregs (P ＜ 0.05).Cox analysis resuhs showed that infiltration of Foxp3 + Tregs was an independent prognostic factor of EHCC (P ＜ 0.05).The positive expression rate of COX-2 in 77 cases of EHCC was 81.8％.COX-2 expression was not significantly associatied with histological grading,TNM stages and lymph node metastasis of EHCC.COX-2 expression was positively related with stromal Tregs infiltration (r =4.89,P ＜ 0.05).Conclusion The number of infiltrated Foxp3 + Tregs in EHCC tissue is negatively related with overall survival of the patients and is an independent prognosis factor of EHCC.COX-2 protein expression may contribute to Foxp3 + Tregs infiltration in cancer stroma.