CAJ | 학술논문

目的探讨组蛋白赖氨酸去甲基化酶1 (LSD1)在食管癌组织中的表达及其意义.方法采用回顾性队列研究方法,调查103例食管癌患者的临床资料;应用免疫组织化学法分别检测食管癌组织和癌旁组织中LSD1的表达.结果免疫组织化学结果显示,在103例食管癌组织中均检测到LSD1的表达,LSD1在癌旁组织以弱表达为主.LSD1主要表达在肿瘤细胞的胞核部位,根据H-score评分及统计学分析结果显示,肿瘤大小≥3.5 cm组中LSD1高表达的比率与肿瘤大小＜3.5 cm组相比差异有统计学意义(16.1％比0.6％,P＜0.05);淋巴结转移组中LSD1高表达的比率显著高于未发生淋巴结转移组(24.5％比3.7％,P＜0.05);远处转移组中LSD1高表达的比率与未发生远处转移组相比差异有统计学意义(31.2％比10.3％,P＜0.05);趋势检验结果显示,随着TNM分期增加LSD1高表达比率显著增高(P＜0.05);Log-rank生存分析结果显示,LSD1高表达的患者,其生存率显著低于低表达的患者[风险比(HR)=2.12,95％可信区间(CI):1.15 ～3.89,P＜0.05];将患者性别、年龄、肿瘤大小、TNM分期及SD1表达水平纳入Cox模型,结果显示只有肿瘤大小(HR=1.98,95％CI:1.16～3.37,P＜0.05)、TNM分期(HR=1.74,95％ CI:1.05～2.88,P＜0.05)可以作为独立的预后风险预测因素.结论食管癌发生与发展过程中存在LSD1异常表达,食管癌组织中LSD1的表达水平与患者的肿瘤进展、转移及预后有关.
목적 탐토조단백뢰안산거갑기화매1 (LSD1)재식관암조직중적표체급기의의.방법 채용회고성대렬연구방법,조사103례식관암환자적림상자료;응용면역조직화학법분별검측식관암조직화암방조직중LSD1적표체.결과 면역조직화학결과현시,재103례식관암조직중균검측도LSD1적표체,LSD1재암방조직이약표체위주.LSD1주요표체재종류세포적포핵부위,근거H-score평분급통계학분석결과현시,종류대소≥3.5 cm조중LSD1고표체적비솔여종류대소＜3.5 cm조상비차이유통계학의의(16.1％비0.6％,P＜0.05);림파결전이조중LSD1고표체적비솔현저고우미발생림파결전이조(24.5％비3.7％,P＜0.05);원처전이조중LSD1고표체적비솔여미발생원처전이조상비차이유통계학의의(31.2％비10.3％,P＜0.05);추세검험결과현시,수착TNM분기증가LSD1고표체비솔현저증고(P＜0.05);Log-rank생존분석결과현시,LSD1고표체적환자,기생존솔현저저우저표체적환자[풍험비(HR)=2.12,95％가신구간(CI):1.15 ～3.89,P＜0.05];장환자성별、년령、종류대소、TNM분기급SD1표체수평납입Cox모형,결과현시지유종류대소(HR=1.98,95％CI:1.16～3.37,P＜0.05)、TNM분기(HR=1.74,95％ CI:1.05～2.88,P＜0.05)가이작위독립적예후풍험예측인소.결론 식관암발생여발전과정중존재LSD1이상표체,식관암조직중LSD1적표체수평여환자적종류진전、전이급예후유관.
Objectve To investigate the lysine specific demethylase 1 (LSD1) expression in human esophageal cancer tissues and its clinical significance.Methods By using the method of retrospective cohort study,the clinical data of 103 patients with esophageal cancer were analyzed.The immunohistochemistry was used to detect the LSD1 expression in esophageal cancer tissues and adjacent normal tissues.The clinical significance of LSD1 expression in esophageal cancer tissues was also analyzed.Results The immunohistochemistry results showed that LSD1 immunolocalization was found in all 103 cases of esophageal cancer tissues,while weak LSD1 staining was found in 5 cases of adjacent normal tissues.Based on the H-score evaluation system and the statistical analysis,we found that in the subgroup of tumor size ≥3.5 cm,the ratio of high LSD1 expression was significantly higher than that in the subgroup of tumor size ＜ 3.5 cm (16.1％ vs.0.6％,P ＜0.05),in the subgroup of nodal metastasis,the ratio of high LSD1 expression was significantly higher than that in the subgroup of without nodal metastasis (24.5％ vs.3.7％,P ＜0.05),in the subgroup of distant metastasis,the ratio of high LSD1 expression was significantly higher than that in the subgroup of without distant metastasis (31.2％ vs.10.3％,P ＜0.05).The chi-square test for trend results showed that the LSD1 expression level was increased in patients with advanced TNM stage (P ＜ 0.05).The Log-rank survival analysis showed that the overall survival rate of the patients with higher LSD1 expression level was significantly lower than that in those patients with lower LSD1 expression level [P＜0.05,Hazard ratio (HR) =2.12,95％ confidence interval (CI) 1.15-3.89].The COX model analysis showed that the tumor size (P ＜ 0.05,HR =1.98,95％ CI:1.16-3.37) and the TNM stage (P ＜0.05,HR =1.74,95％ CI:1.05-2.88) could be used as independent risk factors in predicting esophageal cancer patient' s prognosis,respectivelyConclusion The abnormal expression of LSD1 could be found in the oncogenesis and progression of esophageal cancer.The LSD1 expression level was significantly associated with tumor progression,metastasis,and prognosis of esophageal cancer patients,and could be used as a marker for predicting prognosis of this malignancy.