CAJ | 학술논문

目的探讨特发性草酸钙结石患儿的肠内产甲酸草酸杆菌的定殖以及与24h尿草酸的关系.方法应用聚合酶链反应(PCR)检测20例特发性草酸钙结石患儿(2～18岁)的大便样本中的产甲酸草酸杆菌的存在.根据年龄、性别匹配20名健康儿童作为对照组.同时,留取这些儿童的24h尿并使用离子色谱法测定尿草酸含量.结果在结石组中,6例检出产甲酸草酸杆菌(30％).与正常对照组比较,其定殖率差异无统计学意义(40％,P＞0.05).在结石组中,检测到肠道定殖产甲酸草酸杆菌的患儿的24 h尿草酸排泄要明显低于未检测到定殖的患儿[(0.79±0.29) mmol/(kg·24 h)比(1.16 ±0.32) mmol/(kg·24 h),P＜0.05].结论特发性草酸钙结石患儿的高草酸尿排泄可能是因为肠道内产甲酸草酸杆菌缺乏导致.
목적 탐토특발성초산개결석환인적장내산갑산초산간균적정식이급여24h뇨초산적관계.방법 응용취합매련반응(PCR)검측20례특발성초산개결석환인(2～18세)적대편양본중적산갑산초산간균적존재.근거년령、성별필배20명건강인동작위대조조.동시,류취저사인동적24h뇨병사용리자색보법측정뇨초산함량.결과 재결석조중,6례검출산갑산초산간균(30％).여정상대조조비교,기정식솔차이무통계학의의(40％,P＞0.05).재결석조중,검측도장도정식산갑산초산간균적환인적24 h뇨초산배설요명현저우미검측도정식적환인[(0.79±0.29) mmol/(kg·24 h)비(1.16 ±0.32) mmol/(kg·24 h),P＜0.05].결론 특발성초산개결석환인적고초산뇨배설가능시인위장도내산갑산초산간균결핍도치.
Objective To study the colonization with oxalobacter formigenes in pediatric urolithiasis and the relationship with 24 h urinary oxalate excretion.Methods The presence of oxalobacter formigenes stool samples of 20 children and adolescents (aged 2-18 years) with idiopathic calcium urolithiasis was assessed using polymerase chain reaction (PCR) method.Twenty healthy,age-and sex-matched subjects served as controls.Simultaneously,urinary oxalate excretion was measured by ion chromatography in this group.Resuts Oxalobacter formigenes was found in 6/20 patients (30％).In controls,frequency of colonization had no statistically significant difference (40％,P ＞0.05).The 24 h urinary oxalate excretion in patients colonized with oxalobacter formigenes was significandy lower than in non-colonized patients [(0.79 ± 0.29) mmol/(kg·24 h) vs.(1.16 ± 0.32) mmol/(kg· 24 h),P ＜ 0.05].Conclusion Higher urinary oxalate excretion in children with calcium urolithiasis may be a result of the absence of oxalobacter formigenes.