北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2015年
5期
5-6,41
,共3页
HPLC%桂枝茯苓胶囊%丹皮酚%芍药苷%苦杏仁苷
HPLC%桂枝茯苓膠囊%丹皮酚%芍藥苷%苦杏仁苷
HPLC%계지복령효낭%단피분%작약감%고행인감
HPLC%Guizhifuling capsule%Dan phenol%Paeoniflorin%Peach kernel
目的：建立HPLC法同时测定桂枝茯苓胶囊中丹皮酚、芍药苷和苦杏仁苷的含量。方法：采用HPLC法测定桂枝茯苓胶囊中丹皮酚、芍药苷和苦杏仁苷的含量。采用C18柱，流动相为甲醇∶水（55∶45）洗脱，流速为1.0ml·min-1，检测波长为274nm，柱温为30℃，进样体积为10μL。结果：线性范围分别为0.198～3.166μg（r=0.9998）、0.195～3.128μg（r=0.9996）、0.197～30158（r=0.9997）。平均加样回收率分别为99.81%（RSD=2.48%）、99.69%（RSD=1.47%）、99.79%（RSD=2.12%）。结论：本研究建立的方法简便，结果稳定可靠，可用于桂枝茯苓胶囊的质量控制。
目的：建立HPLC法同時測定桂枝茯苓膠囊中丹皮酚、芍藥苷和苦杏仁苷的含量。方法：採用HPLC法測定桂枝茯苓膠囊中丹皮酚、芍藥苷和苦杏仁苷的含量。採用C18柱，流動相為甲醇∶水（55∶45）洗脫，流速為1.0ml·min-1，檢測波長為274nm，柱溫為30℃，進樣體積為10μL。結果：線性範圍分彆為0.198～3.166μg（r=0.9998）、0.195～3.128μg（r=0.9996）、0.197～30158（r=0.9997）。平均加樣迴收率分彆為99.81%（RSD=2.48%）、99.69%（RSD=1.47%）、99.79%（RSD=2.12%）。結論：本研究建立的方法簡便，結果穩定可靠，可用于桂枝茯苓膠囊的質量控製。
목적：건립HPLC법동시측정계지복령효낭중단피분、작약감화고행인감적함량。방법：채용HPLC법측정계지복령효낭중단피분、작약감화고행인감적함량。채용C18주，류동상위갑순∶수（55∶45）세탈，류속위1.0ml·min-1，검측파장위274nm，주온위30℃，진양체적위10μL。결과：선성범위분별위0.198～3.166μg（r=0.9998）、0.195～3.128μg（r=0.9996）、0.197～30158（r=0.9997）。평균가양회수솔분별위99.81%（RSD=2.48%）、99.69%（RSD=1.47%）、99.79%（RSD=2.12%）。결론：본연구건립적방법간편，결과은정가고，가용우계지복령효낭적질량공제。
Objective To establish an HPLC method for Simultaneous content determination of dan phenol, paeoniflorin and peach kernel in guizhifuling capsule. Methods The content of dan phenol, paeoniflorin and peach kernel in guizhifuling capsule was determinated by HPLC method. The chromatographic condition was C18 column; the mobile phase was methanol-water (55∶45)elution; the flow rate was 1.0ml·min-1; the detection wavelength was 274nm; the column temperature of 30℃; the sample volume was 10μl. Results The linear range were 0.198~3.166μg(r=0.9998), 0.195~3.128μg(r=0.9996), 0.197~30158(r=0.9997)respectively. The average sample recovery rate was 99.81%(RSD=2.48%),99.69% (RSD=1.47%), 99.79%(RSD=2.12%) respectively. Conclusion The established methods is simple by this research, the results is stable and reliable, and can be used for the quality control of guizhifuling capsule.
