CAJ | 학술논문

目的：对战士突出椎间盘髓核细胞进行体外培养，并研究髓核细胞的形态学和生长活性。方法从战士髓核组织分离培养髓核细胞并传代，取传1～7代细胞作为研究对象，光镜和电镜下观察细胞形态，并对细胞进行生长计数测定。结果战士髓核细胞可在体外培养传代，前3代细胞形态良好，增殖力强。结论战士突出椎间盘髓核细胞体外培养成功，前3代细胞可用于细胞学和组织工程学研究。
목적：대전사돌출추간반수핵세포진행체외배양，병연구수핵세포적형태학화생장활성。방법종전사수핵조직분리배양수핵세포병전대，취전1～7대세포작위연구대상，광경화전경하관찰세포형태，병대세포진행생장계수측정。결과전사수핵세포가재체외배양전대，전3대세포형태량호，증식력강。결론전사돌출추간반수핵세포체외배양성공，전3대세포가용우세포학화조직공정학연구。
Objective To investigate the morphology and growth kinetics of the cells derived from the soldier herniated nucleus pulposus tissue.Methods The cells derived from the soldier herniated nucleus pulposus were cultured in vitro.Cells of 1~7 passage were taken as experimental objects.The cell morphology was observed by Optical microscope and Electron Microscope.And the growth kinetics was measured.Results The cells isolated from soldier herniated nucleus pulposus were cultured and passaged successfully,the cell morphology was well and the proliferative ability was vigorous before Passage 3.Conclusion The cells derived from the soldier herinated nucleus pulpo-sus was cultured in vitro successfully.The first 3 generation of cells could be used for cytological and tissue engineering study.