中华放射肿瘤学杂志
中華放射腫瘤學雜誌
중화방사종류학잡지
CHINESE JOURNAL OF RADIATION ONCOLOGY
2015年
3期
344-348
,共5页
马茗微%高献书%鱼红亮%亓昕%孙少倩%李晓颖%马元元
馬茗微%高獻書%魚紅亮%亓昕%孫少倩%李曉穎%馬元元
마명미%고헌서%어홍량%기흔%손소천%리효영%마원원
前列腺癌细胞系%冬虫夏草%放射敏感性%雄激素受体%雄激素样作用
前列腺癌細胞繫%鼕蟲夏草%放射敏感性%雄激素受體%雄激素樣作用
전렬선암세포계%동충하초%방사민감성%웅격소수체%웅격소양작용
Prostate cancer cell line%Cordyceps sinensis%Radiosensitivity%Androgen receptor%Androgen-like effects
目的 探讨冬虫夏草雄激素样作用及对人前列腺癌VCaP细胞放射敏感性影响.方法 检测冬虫夏草灌胃后小鼠激素水平及性器官重量指数变化.克隆形成实验方法观察冬虫夏草对VCaP细胞放射敏感性影响.MTS、流式细胞术、裸鼠移植瘤检测冬虫夏草对VCaP细胞(雄激素受体AR阳性)及PC-3细胞(AR阴性)体外及体内增殖能力影响,比较裸鼠血清PSA水平.单因素方差分析或t检验差异.结果 冬虫夏草组裸鼠睾酮水平高于对照组[(8.28±1.94)、(2.08±1.24)ng/ml,P=0.023),前列腺重量指数也增高[(0.53±0.04)、(0.31±0.04) mg/g,P=0.006].放射增敏比(D0值比)为0.80.冬虫夏草处理后VCaP细胞活力高于对照组(1.32±0.07、0.66±0.02,P=0.000),克隆形成能力较对照组增强(57.0％±1.9％、47.0％±0.6％,P=0.005).冬虫夏草组裸鼠VCaP移植瘤有生长加快趋势,血清PSA升高显著[(0.66±0.04)、(0.26±0.06)ng/ml,P=0.000).该浓度冬虫夏草对PC-3细胞无影响.结论 冬虫夏草有雄激素样作用,同时可促进AR阳性的VCaP细胞增殖能力,降低其放射敏感性.
目的 探討鼕蟲夏草雄激素樣作用及對人前列腺癌VCaP細胞放射敏感性影響.方法 檢測鼕蟲夏草灌胃後小鼠激素水平及性器官重量指數變化.剋隆形成實驗方法觀察鼕蟲夏草對VCaP細胞放射敏感性影響.MTS、流式細胞術、裸鼠移植瘤檢測鼕蟲夏草對VCaP細胞(雄激素受體AR暘性)及PC-3細胞(AR陰性)體外及體內增殖能力影響,比較裸鼠血清PSA水平.單因素方差分析或t檢驗差異.結果 鼕蟲夏草組裸鼠睪酮水平高于對照組[(8.28±1.94)、(2.08±1.24)ng/ml,P=0.023),前列腺重量指數也增高[(0.53±0.04)、(0.31±0.04) mg/g,P=0.006].放射增敏比(D0值比)為0.80.鼕蟲夏草處理後VCaP細胞活力高于對照組(1.32±0.07、0.66±0.02,P=0.000),剋隆形成能力較對照組增彊(57.0％±1.9％、47.0％±0.6％,P=0.005).鼕蟲夏草組裸鼠VCaP移植瘤有生長加快趨勢,血清PSA升高顯著[(0.66±0.04)、(0.26±0.06)ng/ml,P=0.000).該濃度鼕蟲夏草對PC-3細胞無影響.結論 鼕蟲夏草有雄激素樣作用,同時可促進AR暘性的VCaP細胞增殖能力,降低其放射敏感性.
목적 탐토동충하초웅격소양작용급대인전렬선암VCaP세포방사민감성영향.방법 검측동충하초관위후소서격소수평급성기관중량지수변화.극륭형성실험방법관찰동충하초대VCaP세포방사민감성영향.MTS、류식세포술、라서이식류검측동충하초대VCaP세포(웅격소수체AR양성)급PC-3세포(AR음성)체외급체내증식능력영향,비교라서혈청PSA수평.단인소방차분석혹t검험차이.결과 동충하초조라서고동수평고우대조조[(8.28±1.94)、(2.08±1.24)ng/ml,P=0.023),전렬선중량지수야증고[(0.53±0.04)、(0.31±0.04) mg/g,P=0.006].방사증민비(D0치비)위0.80.동충하초처리후VCaP세포활력고우대조조(1.32±0.07、0.66±0.02,P=0.000),극륭형성능력교대조조증강(57.0％±1.9％、47.0％±0.6％,P=0.005).동충하초조라서VCaP이식류유생장가쾌추세,혈청PSA승고현저[(0.66±0.04)、(0.26±0.06)ng/ml,P=0.000).해농도동충하초대PC-3세포무영향.결론 동충하초유웅격소양작용,동시가촉진AR양성적VCaP세포증식능력,강저기방사민감성.
Objective To investigate the androgen-like effects of Cordyceps sinensis (CS) and its impact on the radiosensitivity of VCaP prostate cancer cells.Methods The hormone levels and weight index of reproductive organs in mice were determined after gavage with CS.Clonogenic assay was performed to determine the impact of CS on the radiosensitivity of VCaP cells.The 3-(4,5-dimethylthiazol-2-yl)-5 (3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium assay,flow cytometry,and tumor xenografts in nude mice were performed to determine the effects of CS on the proliferation of VCaP cells (androgen receptor-positive) and PC-3 cells (androgen receptor-negative) in vitro and in vivo.Serum prostate-specific antigen (PSA) levels in nude mice were evaluated.Data were analyzed by one-way analysis of variance or ttest.Results The testosterone level and weight of prostate in mice were significantly higher in the CS group than in the control group ((8.28± 1.94) vs.(2.08± 1.24) ng/ml,P=0.023;(0.53±0.04) vs.(0.31 ± 0.04) mg/g,P =0.006).The radiosensitivity enhancement ratio (ratio of D0 values) was 0.80.The viability of VCaP cells was significantly higher in the CS group than in the control group (1.32 ± 0.07 vs.0.66 ±0.02,P=0.000),and colony forming efficiency was significantly enhanced in the CS group than in the control group (57.0％ ± 1.9％ vs.47.0％ ± 0.6％,P =0.005).VCaP tumor xenografts in nude mice were inclined to grow faster in the CS group than in the control group,and the serum PSA level in the CS group was significantly higher than that in the control group ((0.66 ± 0.04) vs.(0.26 ±0.06) ng/ml,P =0.000).However,CS had no effect on PC-3 cells at the working concentration.Conclusions CS has the androgen-like effects.It may also promote the proliferation and reduce the radiosensitivity of androgen receptor-positive VCaP cells.
