CAJ | 학술논문

目的：建立党参饮片中党参炔苷的含量测定方法，为党参饮片质量标准的完善提供参考。方法党参炔苷的含量测定采用HPLC法，C18色谱柱，流动相为乙腈—水（28∶72），流速为1 mL·min－1，柱温为35℃，检测波长为267 nm；水分、总灰分和浸出物按《中国药典》2010版一部标准检测。结果测定10批党参饮片，党参炔苷含量为0．2315～1．0744 mg·g－1；浸出物为57．5％～69．6％。结论党参饮片中党参炔苷的含量与浸出物量之间没有明显的一致性，应建立具体的量化指标，完善质量评价标准。
목적：건립당삼음편중당삼결감적함량측정방법，위당삼음편질량표준적완선제공삼고。방법당삼결감적함량측정채용HPLC법，C18색보주，류동상위을정—수（28∶72），류속위1 mL·min－1，주온위35℃，검측파장위267 nm；수분、총회분화침출물안《중국약전》2010판일부표준검측。결과측정10비당삼음편，당삼결감함량위0．2315～1．0744 mg·g－1；침출물위57．5％～69．6％。결론당삼음편중당삼결감적함량여침출물량지간몰유명현적일치성，응건립구체적양화지표，완선질량평개표준。
Objective To establish a method for the determination of lobetyolin in Codonopsis pieces,and provide reference values for improving quality standards of Codonopsis pieces.Methods Lobetyolin content was determined by HPLC(high pressure liquid chro-matography)method (column conditions:C18 column,mobile phase acetonitrile—water (28∶72),flow rate 1mL·min-1,column tem-perature 35 ℃,detection wavelength 267 nm.The moisture,total ash and extract were detected according to a standard in"The Chinese Pharmacopoeia of 2010 version".Results 10 batches of Codonopsis pieces were tested,and it was found that lobetyolin was 0.231 5 ~1.074 4 mg·g-1 and extract was 57.5%~69.6%.Conclusions There is no obvious consistency between the lobetyo-lin content and the extract amount,It is necessary to establish specific quantitative indicators to improve the criteria of quality eval-uation.