CAJ | 학술논문

目的：建立RP-HPLC法，测定细叶鸢尾中一种具有抗老年痴呆症活性的二氢黄酮：5，2＇，3＇-三羟基-6，7-亚甲二氧基二氢黄酮。方法采用Agilent 1260HC－C18（4．6 mm ×250 mm，5μm）色谱柱，流动相为甲醇—1％冰醋酸梯度洗脱，流速为1 mL ·min－1，检测波长为295 nm，柱温为25℃。结果该二氢黄酮在37．6～188 mg·L －1浓度范围内线性关系良好，相关系数为0．9994；回收率为101．26％（RSD＝1．17％）。测得该活性化合物在细叶鸢尾中的含量非常高，6批药材中的含量为57．70～59．35 mg·g－1。结论该检测方法准确，适用于细叶鸢尾中5，2＇，3＇-三羟基-6，7-亚甲二氧基二氢黄酮的测定。
목적：건립RP-HPLC법，측정세협연미중일충구유항노년치태증활성적이경황동：5，2＇，3＇-삼간기-6，7-아갑이양기이경황동。방법채용Agilent 1260HC－C18（4．6 mm ×250 mm，5μm）색보주，류동상위갑순—1％빙작산제도세탈，류속위1 mL ·min－1，검측파장위295 nm，주온위25℃。결과해이경황동재37．6～188 mg·L －1농도범위내선성관계량호，상관계수위0．9994；회수솔위101．26％（RSD＝1．17％）。측득해활성화합물재세협연미중적함량비상고，6비약재중적함량위57．70～59．35 mg·g－1。결론해검측방법준학，괄용우세협연미중5，2＇，3＇-삼간기-6，7-아갑이양기이경황동적측정。
Objective This paper established a RP-HPLC method for determination of a flavanone exhibiting potential anti Alzheimer's disease activity in Iris tenuifolia.Methods The separation was carried out with an Agilent 1260HC-C18 column (4.6 mm ×250 mm, 5 μm)by gradient elution using methanol-1% acetic acid as mobile phase at a flow rate of 1 mL·min-1 .The detection wavelength was 295 nm and the column temperature was maintained at 25℃.Results Good linear relationship was observed in the range of 37.6~188 mg·L-1(R2 =0.999 4).The recovery rate was 101.26 %(RSD =1.17 %).And the content of this active compound in Iris tenuifolia was very high,which reached 57.70~59.35 mg·g-1 Conclusions The method had the advantages of acuteness and preci-sion,which was suitable for determination of 5,2',3'-trihydroxy-6,7-methylenedioxyflavanone in Iris tenuifolia.