CAJ | 학술논문

目的：观察山奈酚预处理对大鼠心肌缺血再灌注损伤的影响。方法应用Langendorff灌流装置构建大鼠心脏缺血再灌注模型，120只雄性Sprague-Dawley（SD）大鼠随机分为3组，对照组（n＝40），单纯缺血再灌注组（ n＝40）和山奈酚处理组（ n＝40）。心功能指标从Langendorff灌流装置上获取；采用ELISA法检测CK-MB（ Creatine Kinase Isoenzyme）及SOD（ superoxide dismutase）含量；采用TUNEL法检测心肌细胞凋亡。结果山奈酚预处理能显著提高大鼠心脏缺血再灌注后心功能的恢复及心肌组织内SOD的含量，同时显著降低灌流液中CK-MB的含量，减少再灌注后心肌细胞凋亡。结论山奈酚预处理对缺血再灌注后大鼠心脏具有明显的保护作用。
목적：관찰산내분예처리대대서심기결혈재관주손상적영향。방법응용Langendorff관류장치구건대서심장결혈재관주모형，120지웅성Sprague-Dawley（SD）대서수궤분위3조，대조조（n＝40），단순결혈재관주조（ n＝40）화산내분처리조（ n＝40）。심공능지표종Langendorff관류장치상획취；채용ELISA법검측CK-MB（ Creatine Kinase Isoenzyme）급SOD（ superoxide dismutase）함량；채용TUNEL법검측심기세포조망。결과산내분예처리능현저제고대서심장결혈재관주후심공능적회복급심기조직내SOD적함량，동시현저강저관류액중CK-MB적함량，감소재관주후심기세포조망。결론산내분예처리대결혈재관주후대서심장구유명현적보호작용。
Objective To evaluate the effect of kaempferol against myocardial ischemia-reperfusion injury in rats.Methods Application of Langendorff simulated the model of ischemia-reperfusion.One hundred and twenty male SD rats were randomly divided into 3 groups,group A was the control group(n=40);group B was the ischemia-reperfu-sion group(n=40);group C was the kaempferol-treated group(n=40).Myocardial function were recorded at the screen of Langendorff.The levels of CK-MB and SOD were determined by enzyme linked immunosorbent assay( ELISA) ,Cardio-myocyte apoptosis was determined using terminal deoxynucleotidyl nick-end labeling(TUNEL).Results Pretreatment with kaempferol significantly improved the recovery of heart function as well as increased the levels of SOD.However, pretreatment with kaempferol reduced TUNEL-positive cell rate as well as decreased the levels of CK-MB.Conclusion Pretreatment with kaempferol provides cardioprotection in rats with I/R.