CAJ | 학술논문

根据弧菌属细菌（ Vibrio spp．）共有的rpoA基因序列，比对和设计了一对PCR引物，建立了能够快速而准确地检测弧菌属细菌的通用PCR检测方法，该方法对靶标DNA的检测灵敏度为58 fg／μL，对菌液的检测灵敏度为2．7×102 cfu／mL，能够区分该属细菌与其它属的细菌，具有极高的特异性。使用建立的方法对分离自南美白对虾的病原菌进行了检验，并结合16S rDNA序列分析，结果显示待检测病原均为弧菌属的细菌，与测序结果一致，说明该检测方法可用于弧菌属细菌的检测和诊断。
근거호균속세균（ Vibrio spp．）공유적rpoA기인서렬，비대화설계료일대PCR인물，건립료능구쾌속이준학지검측호균속세균적통용PCR검측방법，해방법대파표DNA적검측령민도위58 fg／μL，대균액적검측령민도위2．7×102 cfu／mL，능구구분해속세균여기타속적세균，구유겁고적특이성。사용건립적방법대분리자남미백대하적병원균진행료검험，병결합16S rDNA서렬분석，결과현시대검측병원균위호균속적세균，여측서결과일치，설명해검측방법가용우호균속세균적검측화진단。
A pair of PCR primers was designed according to the consensus sequence of the rpoA of Vibrio and a PCR assay for Vibrio spp.was thus established .The PCR assay could distinguish Vibrio spp from bacteria of other genus with great spe-cificity and the sensitivity of the assay was 58 fg/μL for DNA and 2.7 ×10 2 cfu/mL for Vibrio strain.Pathogens isolated from Penaeus vannmei were identified by established PCR and 16 S rDNA sequencing respectively .The result showed that the two assays had great conformity with each other , which indicated that the developed PCR assay can be used to detect Vibrio spp.