淡水渔业
淡水漁業
담수어업
FRESHWATER FISHERIES
2015年
3期
40-45
,共6页
刘永涛%艾晓辉%杨秋红%胥宁
劉永濤%艾曉輝%楊鞦紅%胥寧
류영도%애효휘%양추홍%서저
超临界CO2 流体%萃取物%中草药%多子水霉(Saprolegniales ferax)%澳大利亚水霉(Saprolegnia australis)
超臨界CO2 流體%萃取物%中草藥%多子水黴(Saprolegniales ferax)%澳大利亞水黴(Saprolegnia australis)
초림계CO2 류체%췌취물%중초약%다자수매(Saprolegniales ferax)%오대리아수매(Saprolegnia australis)
supercritical CO 2 fluid%extracts%Chinese herbal medicines%Saprolegniales ferax%Saprolegnia australis
研究了15种中草药超临界CO2流体萃取技术，从中草药粉碎的粒度、超临界CO2流体萃取仪的萃取温度、萃取压力对中草药提取条件进行了优化，获得了15种中草药超临界CO2流体萃取技术方案；将15种中草药超临界CO2流体萃取物添加到PDA培养基中研究了15种中草药萃取物对多子水霉（ Saprolegniales ferax）和澳大利亚水霉（ Saprolegnia australis）菌丝的抑制作用，结果显示：中草药粉碎成20目时，适宜采用超临界CO2流体萃取仪进行萃取，当超临界CO2流体萃取仪的萃取温度一定的条件下萃取物产率随萃取压力的提高而增加，当萃取压力一定的条件下萃取物产率随萃取温度的增加先增加后降低；15种中草药中川楝子、土槿皮、五倍子、山苍子的萃取物对2种水霉菌菌丝的生长抑制效果相对较好，当萃取物浓度大于200 mg／L时才能较好抑制2种水霉菌菌丝的生长。
研究瞭15種中草藥超臨界CO2流體萃取技術，從中草藥粉碎的粒度、超臨界CO2流體萃取儀的萃取溫度、萃取壓力對中草藥提取條件進行瞭優化，穫得瞭15種中草藥超臨界CO2流體萃取技術方案；將15種中草藥超臨界CO2流體萃取物添加到PDA培養基中研究瞭15種中草藥萃取物對多子水黴（ Saprolegniales ferax）和澳大利亞水黴（ Saprolegnia australis）菌絲的抑製作用，結果顯示：中草藥粉碎成20目時，適宜採用超臨界CO2流體萃取儀進行萃取，噹超臨界CO2流體萃取儀的萃取溫度一定的條件下萃取物產率隨萃取壓力的提高而增加，噹萃取壓力一定的條件下萃取物產率隨萃取溫度的增加先增加後降低；15種中草藥中川楝子、土槿皮、五倍子、山蒼子的萃取物對2種水黴菌菌絲的生長抑製效果相對較好，噹萃取物濃度大于200 mg／L時纔能較好抑製2種水黴菌菌絲的生長。
연구료15충중초약초림계CO2류체췌취기술，종중초약분쇄적립도、초림계CO2류체췌취의적췌취온도、췌취압력대중초약제취조건진행료우화，획득료15충중초약초림계CO2류체췌취기술방안；장15충중초약초림계CO2류체췌취물첨가도PDA배양기중연구료15충중초약췌취물대다자수매（ Saprolegniales ferax）화오대리아수매（ Saprolegnia australis）균사적억제작용，결과현시：중초약분쇄성20목시，괄의채용초림계CO2류체췌취의진행췌취，당초림계CO2류체췌취의적췌취온도일정적조건하췌취물산솔수췌취압력적제고이증가，당췌취압력일정적조건하췌취물산솔수췌취온도적증가선증가후강저；15충중초약중천련자、토근피、오배자、산창자적췌취물대2충수매균균사적생장억제효과상대교호，당췌취물농도대우200 mg／L시재능교호억제2충수매균균사적생장。
Extraction technology was studied by using supercritical CO 2 fluid extraction instrument from 15 Chinese herbal medicines.The extraction conditions were optimized by the particle size of Chinese herbal medicines , the extraction temper-ature of the supercritical CO 2 fluid extraction instrument , the extraction pressure of the supercritical CO 2 fluid extraction in-strument and 15 extracts of Chinese herbal medicines were obtained .The inhibition effects of 15 extracts of Chinese herbal medicines on Saprolegniales ferax mycelium and Saprolegnia australis mycelium were tested by the addition of the extracts of Chinese herbal medicine in the PDA culture medium .The results showed that the Chinese herbal medicines were suitable to be extracted with the supercritical CO 2 fluid extraction instrument , when the Chinese herbal medicines were pulverized to 20 mesh particles.The extracts yield of the Chinese herbal medicines increased with the increase of the extraction tempera -ture on the certain extraction pressure .The extracts yield of the Chinese herbal medicines first increased and then decreased with the increase of the extraction temperature on the certain extraction pressure .The extracts of Fructus Toosendan , Chinense Golden Larch Bark , Galla chinensis , and Litsea Cubeba had better inhibition effects on S.ferax mycelium and S.australis mycelium than the other extracts of Chinese herbal medicines .S.ferax mycelium and Saprolegnia australis myceliumwere well inhibited at the concentration of more than 200 mg/L.
