CAJ | 학술논문

目的：探讨积雪苷霜软膏对兔耳增生性瘢痕组织中Smad4蛋白表达的影响。方法新西兰大耳白兔18只，随机分为正常对照组、模型组、积雪苷霜软膏组。模型组和积雪苷霜软膏组建立兔耳腹侧面增生性瘢痕模型，术后21 d，积雪苷霜软膏组瘢痕局部涂抹积雪苷霜软膏，每天2次，连续用药28 d；正常组和模型组涂抹软膏基质。每周观察并拍照记录瘢痕的生长情况，于术后49 d处死动物，进行病理、羟脯氨酸和Smad4蛋白检测。结果模型组瘢痕以较快速度生长，积雪苷霜软膏组，瘢痕生长显著被抑制。病理检测显示，模型组增生性瘢痕组织纤维化明显，而积雪苷治疗可显著改善纤维化。羟脯氨酸定量显示，增生性瘢痕组织中羟脯氨酸（Hyp）含量显著升高（P＜0．01），而积雪苷治疗显著降低瘢痕组织中Hyp水平（P＜0．01）。免疫印迹结果显示增生性瘢痕组织中Smad4蛋白水平显著上调，而积雪苷治疗显著降低Smad4蛋白表达（P＜0．01）。结论积雪苷霜抗增生性瘢痕作用可能与调控Smad4蛋白表达有关。
목적：탐토적설감상연고대토이증생성반흔조직중Smad4단백표체적영향。방법신서란대이백토18지，수궤분위정상대조조、모형조、적설감상연고조。모형조화적설감상연고조건립토이복측면증생성반흔모형，술후21 d，적설감상연고조반흔국부도말적설감상연고，매천2차，련속용약28 d；정상조화모형조도말연고기질。매주관찰병박조기록반흔적생장정황，우술후49 d처사동물，진행병리、간포안산화Smad4단백검측。결과모형조반흔이교쾌속도생장，적설감상연고조，반흔생장현저피억제。병리검측현시，모형조증생성반흔조직섬유화명현，이적설감치료가현저개선섬유화。간포안산정량현시，증생성반흔조직중간포안산（Hyp）함량현저승고（P＜0．01），이적설감치료현저강저반흔조직중Hyp수평（P＜0．01）。면역인적결과현시증생성반흔조직중Smad4단백수평현저상조，이적설감치료현저강저Smad4단백표체（P＜0．01）。결론적설감상항증생성반흔작용가능여조공Smad4단백표체유관。
Objective To investigate the effects of asiaticoside ointment on Smad4 protein expression in rabbit's hypertrophic scars. Methods The eighteen New Zealand white rabbits were randomly distributed into the control group,the model group and the asiaticosi-de ointment treatment group.The scars were established on the ventral surface of rabbits'ears in the model and asiaticoside ointment treatment group.On post-operative day 21,the scars of asiaticoside ointment treatment group were thinly daubed with asiaticoside oint-ment twice a day for 28 days.Meanwhile,those of the control and model groups were applied with ointment base.Morphological charac-teristics were observed and recorded by a camera every week.All animals were executed and the scars or corresponding skin tissues were harvested for histopathology,hydroxyproline and Smad4 protein detection on post-operative day 49.Results The growth of scars in the model group was more rapid than that of the asiaticoside ointment treatment group.Pathological evaluation shows that representative fibrosis occurred in the scars of the model group,while that was markedly alleviated in the scars of the asiaticoside ointment treatment group.The hydroxyproline content of scars in the model group observably increased compared with that in the control group(P<0.01), while significantly decreased in the asiaticoside ointment treatment group(P<0.01).The expression of Smad4 protein was remarkably up-regulated in hypertrophic scar tissue.Nevertheless,it was notably decreased in asiaticoside ointment treatment(P<0.01 ).Conclu-sions The mechanisms of anti-hypertrophic scar effects of asiaticoside maybe involve inhibiting Smad4 protein expression.