CAJ | 학술논문

目的观察急性肺栓塞大鼠血小板活化标志物GMP-140和CD63的表达及动态变化并探讨其对肺栓塞的诊断价值.方法健康雄性Wistar大鼠40只,随机分为4组:对照组、栓塞后6h组、栓塞后12 h组、栓塞后24 h组,每组10只.将栓子经颈静脉注入,制备大鼠急性肺栓塞模型,对照组注射等量生理盐水.在相应时间点采血分离富含血小板血浆,应用流式细胞术检测GMP-140和CD63的表达.结果血小板活化标志物GMP-140在栓塞后6h、12 h、24 h组中表达阳性率分别为(2.031±0.417)％、(1.311±0.387)％、(0.929±0.244)％,均高于对照组[(0.538士0.341)％,P＜0.01];血小板活化标志物CD63在栓塞后6h、12 h、24 h组中表达阳性率分别为(2.642±1.394)％、(1.850±0.982)％、(0.887±0.290)％,均高于对照组[(0.404±0.305)％,P＜0.01];均以栓塞后6h表达最显著.其中GMP-140的表达栓塞后6h组同栓塞后12h、24 h组比较差异有统计学意义(P＜0.01),栓塞后12 h组与栓塞后24 h组间比较差异无统计学意义(P＞0.01);CD63的表达栓塞后12h组同栓塞后24 h组间比较差异无统计学意义(P＞0.01),栓塞后6h组与栓塞后24 h组间比较差异有统计学意义(P＜0.01).结论实验组大鼠不同时间GMP-140和CD63均升高,可以为肺栓塞的诊断提供依据.
목적 관찰급성폐전새대서혈소판활화표지물GMP-140화CD63적표체급동태변화병탐토기대폐전새적진단개치.방법 건강웅성Wistar대서40지,수궤분위4조:대조조、전새후6h조、전새후12 h조、전새후24 h조,매조10지.장전자경경정맥주입,제비대서급성폐전새모형,대조조주사등량생리염수.재상응시간점채혈분리부함혈소판혈장,응용류식세포술검측GMP-140화CD63적표체.결과 혈소판활화표지물GMP-140재전새후6h、12 h、24 h조중표체양성솔분별위(2.031±0.417)％、(1.311±0.387)％、(0.929±0.244)％,균고우대조조[(0.538사0.341)％,P＜0.01];혈소판활화표지물CD63재전새후6h、12 h、24 h조중표체양성솔분별위(2.642±1.394)％、(1.850±0.982)％、(0.887±0.290)％,균고우대조조[(0.404±0.305)％,P＜0.01];균이전새후6h표체최현저.기중GMP-140적표체전새후6h조동전새후12h、24 h조비교차이유통계학의의(P＜0.01),전새후12 h조여전새후24 h조간비교차이무통계학의의(P＞0.01);CD63적표체전새후12h조동전새후24 h조간비교차이무통계학의의(P＞0.01),전새후6h조여전새후24 h조간비교차이유통계학의의(P＜0.01).결론 실험조대서불동시간GMP-140화CD63균승고,가이위폐전새적진단제공의거.
Objective To observe the expressions and the dynamic change of platelet activation markers GMP140 and CD63 in rats with acute pulmonary embolism,so that to explore the value of the markers in early diagnosis and treatment of pulmonary embolism.Methods Forty healthy male Wistar rats were divided randomly into four groups as follows:six hours after embolism group,12 hours after embolism group,24 hours after embolism group,and control group.Each group contained ten rats.Emboli was injected through the jugular vein to establish the model of pulmonary embolism,while the same amount of normal saline was injected through the jugular vein to the control group.The platelet-rich plasma was obtained from blood sample collected from experimental rats and control rats.The expressions of platelet-associated GMP-140 and CD63 in platelet-rich plasma were detected by flow cytometry.Results The expression of platelet activation marker GMP-140 in six hours,12 hours,24 hours after embllism groups [(2.031±0.417)％,(1.311±0.387)％,(0.929±0.244)％] was higher than that in control group [(0.538± 0.341)％,P ＜0.01].The expression of platelet activation marker CD63 in six hours,12 hours,24 hours after embllism groups [(2.642 ± 1.394) ％,(1.850 ± 0.982) ％,(0.887 ± 0.290)％] was higher than that in control group [(0.404±0.305)％,P ＜0.01].The expressions of the markers were most significant in six hours after embolism group.The expression of GMP-140 in six hours group was significant higher than that in 12 hours group and 24 hours group (P ＜0.01),while there was no difference in the expression of GMP-140 and CD63 between 12 hours group and 24 hours group (P ＞ 0.01).There was statistical difference in the expression of CD63 between six hours group and 24 hours group (P ＜0.01).Conclusions The expressions of GMP-140 and CD63 increase at different time points of the experimental groups.The results can provide reference for the assess of pulmonary thromboembolism.