浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2015年
5期
427-430
,共4页
李博%毛颖民%杨建生%赵仲生
李博%毛穎民%楊建生%趙仲生
리박%모영민%양건생%조중생
胃肿瘤%浸润转移%基质金属蛋白酶-11
胃腫瘤%浸潤轉移%基質金屬蛋白酶-11
위종류%침윤전이%기질금속단백매-11
gastric neoplasma%neoplasma invasion%matrix metalloproteinases-11
目的：观察MMP-11蛋白在胃癌组织的表达。方法采用免疫组织化学技术检测436份胃癌组织及92份邻近正常胃黏膜组织石蜡标本MMP-11表达情况。结果 MMP-11在正常胃黏膜组织的表达低于胃癌组织（0 vs 47.5%，χ2=73.424，P<0.01）。MMP-11表达与胃癌患者肿瘤大小、分化、侵犯深度、分期、淋巴转移、远处转移、脉管侵犯、Lauren分型相关（P<0.05），而与组织类型、性别无关。肿瘤≥5cm者MMP-11阳性表达率62.8%，明显高于肿瘤<5cm者的36.7%（χ2=28.781，P<0.001）；弥漫型者MMP-11阳性表达率明显高于肠型者（85.9%比10.7%，χ2=246.751，P<0.001）；T3、T4期者MMP-11阳性表达率分别为58.6%、96.2%，明显高于T1、T2期的10.5%、30.3%（χ2=80.969， P<0.001）；Ⅲ期、Ⅳ期胃癌患者MMP-11阳性表达率分别为68.8%、95.7%，高于Ⅰ期、Ⅱ期患者的7.8%、14.4%（χ2=198.170，P<0.001）；脉管侵犯者 MMP-11阳性表达率明显高于无脉管侵犯者（70.0%比16.4%，χ2=122.193，P<0.001）；淋巴结转移胃癌患者的MMP-11阳性表达率为68.9%，高于无淋巴结转移的12.7%（χ2=130.382，P<0.001）；有远处转移者的MMP-11阳性表达率95.1%，高于无远处转移者的39.7%（χ2=64.455，P<0.001）。根据多因素Logistic回归分析，MMP11的表达在TNM分期高、脉管侵犯、有远处转移的患者中发生率高。结论 MMP-11或参与了胃癌的浸润转移进程，检测MMP-11的表达可作为判断胃癌生物学行为的重要参考指标。
目的：觀察MMP-11蛋白在胃癌組織的錶達。方法採用免疫組織化學技術檢測436份胃癌組織及92份鄰近正常胃黏膜組織石蠟標本MMP-11錶達情況。結果 MMP-11在正常胃黏膜組織的錶達低于胃癌組織（0 vs 47.5%，χ2=73.424，P<0.01）。MMP-11錶達與胃癌患者腫瘤大小、分化、侵犯深度、分期、淋巴轉移、遠處轉移、脈管侵犯、Lauren分型相關（P<0.05），而與組織類型、性彆無關。腫瘤≥5cm者MMP-11暘性錶達率62.8%，明顯高于腫瘤<5cm者的36.7%（χ2=28.781，P<0.001）；瀰漫型者MMP-11暘性錶達率明顯高于腸型者（85.9%比10.7%，χ2=246.751，P<0.001）；T3、T4期者MMP-11暘性錶達率分彆為58.6%、96.2%，明顯高于T1、T2期的10.5%、30.3%（χ2=80.969， P<0.001）；Ⅲ期、Ⅳ期胃癌患者MMP-11暘性錶達率分彆為68.8%、95.7%，高于Ⅰ期、Ⅱ期患者的7.8%、14.4%（χ2=198.170，P<0.001）；脈管侵犯者 MMP-11暘性錶達率明顯高于無脈管侵犯者（70.0%比16.4%，χ2=122.193，P<0.001）；淋巴結轉移胃癌患者的MMP-11暘性錶達率為68.9%，高于無淋巴結轉移的12.7%（χ2=130.382，P<0.001）；有遠處轉移者的MMP-11暘性錶達率95.1%，高于無遠處轉移者的39.7%（χ2=64.455，P<0.001）。根據多因素Logistic迴歸分析，MMP11的錶達在TNM分期高、脈管侵犯、有遠處轉移的患者中髮生率高。結論 MMP-11或參與瞭胃癌的浸潤轉移進程，檢測MMP-11的錶達可作為判斷胃癌生物學行為的重要參攷指標。
목적：관찰MMP-11단백재위암조직적표체。방법채용면역조직화학기술검측436빈위암조직급92빈린근정상위점막조직석사표본MMP-11표체정황。결과 MMP-11재정상위점막조직적표체저우위암조직（0 vs 47.5%，χ2=73.424，P<0.01）。MMP-11표체여위암환자종류대소、분화、침범심도、분기、림파전이、원처전이、맥관침범、Lauren분형상관（P<0.05），이여조직류형、성별무관。종류≥5cm자MMP-11양성표체솔62.8%，명현고우종류<5cm자적36.7%（χ2=28.781，P<0.001）；미만형자MMP-11양성표체솔명현고우장형자（85.9%비10.7%，χ2=246.751，P<0.001）；T3、T4기자MMP-11양성표체솔분별위58.6%、96.2%，명현고우T1、T2기적10.5%、30.3%（χ2=80.969， P<0.001）；Ⅲ기、Ⅳ기위암환자MMP-11양성표체솔분별위68.8%、95.7%，고우Ⅰ기、Ⅱ기환자적7.8%、14.4%（χ2=198.170，P<0.001）；맥관침범자 MMP-11양성표체솔명현고우무맥관침범자（70.0%비16.4%，χ2=122.193，P<0.001）；림파결전이위암환자적MMP-11양성표체솔위68.9%，고우무림파결전이적12.7%（χ2=130.382，P<0.001）；유원처전이자적MMP-11양성표체솔95.1%，고우무원처전이자적39.7%（χ2=64.455，P<0.001）。근거다인소Logistic회귀분석，MMP11적표체재TNM분기고、맥관침범、유원처전이적환자중발생솔고。결론 MMP-11혹삼여료위암적침윤전이진정，검측MMP-11적표체가작위판단위암생물학행위적중요삼고지표。
Objective To investigate the expression of MMP-11 in gastric cancer. Methods MMP-11 expres-sion in sample tissues from 436 cases with gastric cancer and 92 cases of cancer adjacent gastric mucosa was de-tected immunohisochemically. Results MMP-11 expression in cancer adjacent gastric mucosa was significantly low-er than that in gastric cancer tissues (0 vs 47.5% χ2=73.424, P<0.01). MMP-11 expression was related to tumor size, differentiation, depth of invasion, stage, lymph node metastasis, distant metastasis, vascular invasion, and Lau-ren classification of gastric cancer(P<0.05), but was not associated with histologic type and gender of patients(P>0.05). MMP-11 positive rate in gastric cancer patients with tumor size ≥5cm was significantly higher than that in patients with tumor size <5cm (62.8% vs 36.7%; χ2=28.781, P<0.001); that in patients with diffusing type cancer increased to 85.0% as compared with 10.7% in patients with intestinal-type cancer (χ2=246.751, P<0.001); that in patients at stage T3 and T4 was 58.6% and 96.2%, respectively, which were higher than that in patients at stage T1 and T2(10.5% and 30.3%, χ2=80.969, P<0.001); that in patients with stage Ⅲ, Ⅳ cancer(68.8%, 95.7%)were significantly higher than that in patient with stage Ⅰ, II cancer (7.8%, 14.4%; χ2=198.170, P<0.001); that in patients with vascular invasion was 70.0% as compared with 16.4% of patients without vascular invasion MMP-11 (χ2=122.193, P<0.001); that in patients with lymph node metastasis was higher than that in patients without lymph node metastasis(68.9% vs 12.7%; χ2=130.382, P<0.001); that in patients with distant metastasis increased to 95.1%as compared with 39.7% in patients without distant metastasis(χ2=64.455, P<0.001). Multi-factor logistic regression analysis showed that MMP-11 high expression was seen in patients with high TNM staging, vascular invasion, or distant metastasis. Conclusion MMP-11 may involve in process of invasion and metastasis of gastric cancer. MMP-11 could be used as an important reference indicator for evaluation of biological behavior of gastric cancer.
