中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
19期
1488-1492
,共5页
马龙%吴昆%刘坤%顾硕%王毅%徐宗源%于湘友%孟峻嵩
馬龍%吳昆%劉坤%顧碩%王毅%徐宗源%于湘友%孟峻嵩
마룡%오곤%류곤%고석%왕의%서종원%우상우%맹준숭
肾功能衰竭,急性%再灌注损伤%生物学标记%微小RNA
腎功能衰竭,急性%再灌註損傷%生物學標記%微小RNA
신공능쇠갈,급성%재관주손상%생물학표기%미소RNA
Kidney failure,acute%Reperfusion injury%Biological markers%microRNA
目的 观察大鼠肾缺血再灌注损伤中miRNA表达谱变化及筛选出的miRNAs在肾缺血再灌注损伤肾组织中时间变化以寻找急性肾损伤早期诊断和预后评价的目标miRNAs.方法 利用miRNA微流体芯片技术检测大鼠正常肾脏及缺血再灌注损伤肾脏miRNA表达谱的差异,并使用实时定量聚合酶链反应(qRT-PCR)进行验证.筛选出差异最为显著miRNAs,并分析其在再灌注后水平的时间变化及和肾损伤的关系.结果 有明显差异表达的miRNA共有21个,其中有5个miRNAs差异最为显著;miR-17-5p、miR-21和miR-106a肾组织表达水平和芯片结果保持一致.缺血再灌注早期(再灌注4h后)肾组织miR-17-5p水平即出现明显升高(P＜0.05),早于BUN和NGAL明显变化时间;缺血30 min组和缺血20 min组分别于再灌注12 h和24h后其miRNA-21、miR-106a水平开始显著升高(P＜0.01),和血BUN和NGAL水平变化趋势一致.结论 miR-17-5 p、miR-21、miR-106a在肾缺血再灌注损伤的不同阶段及不同缺血时间会发生不同程度的表达量变化,且miR-17-5p早于血NGAL及BUN开始出现升高,可能是一种较之BUN和NGAL更为优越的评估AKI的有效标志物.
目的 觀察大鼠腎缺血再灌註損傷中miRNA錶達譜變化及篩選齣的miRNAs在腎缺血再灌註損傷腎組織中時間變化以尋找急性腎損傷早期診斷和預後評價的目標miRNAs.方法 利用miRNA微流體芯片技術檢測大鼠正常腎髒及缺血再灌註損傷腎髒miRNA錶達譜的差異,併使用實時定量聚閤酶鏈反應(qRT-PCR)進行驗證.篩選齣差異最為顯著miRNAs,併分析其在再灌註後水平的時間變化及和腎損傷的關繫.結果 有明顯差異錶達的miRNA共有21箇,其中有5箇miRNAs差異最為顯著;miR-17-5p、miR-21和miR-106a腎組織錶達水平和芯片結果保持一緻.缺血再灌註早期(再灌註4h後)腎組織miR-17-5p水平即齣現明顯升高(P＜0.05),早于BUN和NGAL明顯變化時間;缺血30 min組和缺血20 min組分彆于再灌註12 h和24h後其miRNA-21、miR-106a水平開始顯著升高(P＜0.01),和血BUN和NGAL水平變化趨勢一緻.結論 miR-17-5 p、miR-21、miR-106a在腎缺血再灌註損傷的不同階段及不同缺血時間會髮生不同程度的錶達量變化,且miR-17-5p早于血NGAL及BUN開始齣現升高,可能是一種較之BUN和NGAL更為優越的評估AKI的有效標誌物.
목적 관찰대서신결혈재관주손상중miRNA표체보변화급사선출적miRNAs재신결혈재관주손상신조직중시간변화이심조급성신손상조기진단화예후평개적목표miRNAs.방법 이용miRNA미류체심편기술검측대서정상신장급결혈재관주손상신장miRNA표체보적차이,병사용실시정량취합매련반응(qRT-PCR)진행험증.사선출차이최위현저miRNAs,병분석기재재관주후수평적시간변화급화신손상적관계.결과 유명현차이표체적miRNA공유21개,기중유5개miRNAs차이최위현저;miR-17-5p、miR-21화miR-106a신조직표체수평화심편결과보지일치.결혈재관주조기(재관주4h후)신조직miR-17-5p수평즉출현명현승고(P＜0.05),조우BUN화NGAL명현변화시간;결혈30 min조화결혈20 min조분별우재관주12 h화24h후기miRNA-21、miR-106a수평개시현저승고(P＜0.01),화혈BUN화NGAL수평변화추세일치.결론 miR-17-5 p、miR-21、miR-106a재신결혈재관주손상적불동계단급불동결혈시간회발생불동정도적표체량변화,차miR-17-5p조우혈NGAL급BUN개시출현승고,가능시일충교지BUN화NGAL경위우월적평고AKI적유효표지물.
Objective Ischemia-reperfusion (I/R) is a main cause of acute kidney injury (AKI).The renal expression profiles of microRNA (miRNA) and time course of their changes after renal I/R were explored to screen acute AKI prognostic-related microRNAs and biomarkers.Methods The expression profile of miRNA was analyzed for detecting miRNAs in kidney after renal I/R injury.Real-time polymerase chain reaction (PCR) was performed to validate the results of microarray.And the relationship was examined between kidney injury and time course of changes in selected miRNAs.Results Twenty-one miRNAs were differentially expressed in kidney of rats with renal I/R injury.And 5 miRNAs had prominent differences.miR-17-5p,miR-21 and miR-106a were selected for further confirmation by quantitative realtime-PCR.And the results were consistent with those of microarry.During early stage (4 h) after I/R,the expression level of miR-17-5p significantly increased (P ＜ 0.05).And it occurred earlier than those of BUN level and plasma concentration of neutrophil gelatinase-associated lipocalin (NGAL).Renal expressions of miR-21 and miR-106a were significantly elevated in ischemia 20 min and 30 min groups at 12 h and 24 h post-reperfusion (P ＜ 0.01).And the trend was in accordance with those of BUN and NGAL.Conclusions miR-21,miR-17-5p and miR-106a are differentially expressed during different phases of renal I/R injury.And miR-17-5p is more sensitive than BUN and NGAL so that it is a more ideal biomarker for AKI.
