中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
14期
1078-1082
,共5页
黄小燕%冯琨%刘丹%金雪玲%张剑%罗庆
黃小燕%馮琨%劉丹%金雪玲%張劍%囉慶
황소연%풍곤%류단%금설령%장검%라경
鼻炎%鼻窦炎%pendrin%黏蛋白类
鼻炎%鼻竇炎%pendrin%黏蛋白類
비염%비두염%pendrin%점단백류
Rhinitis%Sinusitis%pendrin%Mucins
目的 探讨pendrin在慢性鼻-鼻窦炎(CRS)中的表达及与黏蛋白5AC(MUC5AC)的相关性.方法 取伴或无息肉的CRS患者上颌窦窦口黏膜各30例分别作为伴息肉CRS组和无息肉CRS组、另取10例单纯性上颌窦囊肿患者的上颌窦口前壁近钩突部位的黏膜作为对照组,采用HE染色观察各组鼻窦黏膜的组织形态学改变,糖原过碘酸雪夫染色(PAS染色)检测各组鼻窦黏膜中总黏蛋白的表达情况,免疫组织化学方法、实时荧光定量PCR法和免疫印迹法检测各组鼻窦黏膜中pendrin和MUC5AC的表达情况.结果 在伴息肉CRS组和无息肉CRS组鼻窦黏膜中均可观察到黏膜增厚,杯状细胞增生,上皮纤毛部分缺失,腺体增生,炎性细胞浸润;pendrin蛋白主要表达于鼻窦黏膜上皮细胞的顶膜,其次为黏膜下腺体细胞;MUC5AC主要表达于鼻窦黏膜上皮的杯状细胞和假复层纤毛柱状细胞,少量表达于黏膜下腺体细胞.伴息肉CRS组和无息肉CRS组鼻窦黏膜中总黏蛋白、pendrin蛋白、MUC5AC阳性表达率(86.7％和80.0％比20.0％、73.3％和70.0％比10.0％、83.3％和76.7％比20.0％)、pendrin的mRNA及蛋白的相对表达水平(1.685±0.111和1.537±0.262比1.012±0.167及2.066±0.188、1.996±0.153比1.000±0.169)、MUC5AC的mRNA相对表达水平(1.861±0.718、1.562±0.428比1.004±0.094)均显著高于对照组(均P＜0.05).pendrin mRNA的表达水平与MUC5AC mRNA表达水平呈正相关(r=0.670,P=0.006和r=0.713,P=0.003).结论 在CRS黏膜中表达上调的pendrin与MUCSAC的表达增加有一定关联,前者可能是CRS中黏液高分泌的重要因子.
目的 探討pendrin在慢性鼻-鼻竇炎(CRS)中的錶達及與黏蛋白5AC(MUC5AC)的相關性.方法 取伴或無息肉的CRS患者上頜竇竇口黏膜各30例分彆作為伴息肉CRS組和無息肉CRS組、另取10例單純性上頜竇囊腫患者的上頜竇口前壁近鉤突部位的黏膜作為對照組,採用HE染色觀察各組鼻竇黏膜的組織形態學改變,糖原過碘痠雪伕染色(PAS染色)檢測各組鼻竇黏膜中總黏蛋白的錶達情況,免疫組織化學方法、實時熒光定量PCR法和免疫印跡法檢測各組鼻竇黏膜中pendrin和MUC5AC的錶達情況.結果 在伴息肉CRS組和無息肉CRS組鼻竇黏膜中均可觀察到黏膜增厚,杯狀細胞增生,上皮纖毛部分缺失,腺體增生,炎性細胞浸潤;pendrin蛋白主要錶達于鼻竇黏膜上皮細胞的頂膜,其次為黏膜下腺體細胞;MUC5AC主要錶達于鼻竇黏膜上皮的杯狀細胞和假複層纖毛柱狀細胞,少量錶達于黏膜下腺體細胞.伴息肉CRS組和無息肉CRS組鼻竇黏膜中總黏蛋白、pendrin蛋白、MUC5AC暘性錶達率(86.7％和80.0％比20.0％、73.3％和70.0％比10.0％、83.3％和76.7％比20.0％)、pendrin的mRNA及蛋白的相對錶達水平(1.685±0.111和1.537±0.262比1.012±0.167及2.066±0.188、1.996±0.153比1.000±0.169)、MUC5AC的mRNA相對錶達水平(1.861±0.718、1.562±0.428比1.004±0.094)均顯著高于對照組(均P＜0.05).pendrin mRNA的錶達水平與MUC5AC mRNA錶達水平呈正相關(r=0.670,P=0.006和r=0.713,P=0.003).結論 在CRS黏膜中錶達上調的pendrin與MUCSAC的錶達增加有一定關聯,前者可能是CRS中黏液高分泌的重要因子.
목적 탐토pendrin재만성비-비두염(CRS)중적표체급여점단백5AC(MUC5AC)적상관성.방법 취반혹무식육적CRS환자상합두두구점막각30례분별작위반식육CRS조화무식육CRS조、령취10례단순성상합두낭종환자적상합두구전벽근구돌부위적점막작위대조조,채용HE염색관찰각조비두점막적조직형태학개변,당원과전산설부염색(PAS염색)검측각조비두점막중총점단백적표체정황,면역조직화학방법、실시형광정량PCR법화면역인적법검측각조비두점막중pendrin화MUC5AC적표체정황.결과 재반식육CRS조화무식육CRS조비두점막중균가관찰도점막증후,배상세포증생,상피섬모부분결실,선체증생,염성세포침윤;pendrin단백주요표체우비두점막상피세포적정막,기차위점막하선체세포;MUC5AC주요표체우비두점막상피적배상세포화가복층섬모주상세포,소량표체우점막하선체세포.반식육CRS조화무식육CRS조비두점막중총점단백、pendrin단백、MUC5AC양성표체솔(86.7％화80.0％비20.0％、73.3％화70.0％비10.0％、83.3％화76.7％비20.0％)、pendrin적mRNA급단백적상대표체수평(1.685±0.111화1.537±0.262비1.012±0.167급2.066±0.188、1.996±0.153비1.000±0.169)、MUC5AC적mRNA상대표체수평(1.861±0.718、1.562±0.428비1.004±0.094)균현저고우대조조(균P＜0.05).pendrin mRNA적표체수평여MUC5AC mRNA표체수평정정상관(r=0.670,P=0.006화r=0.713,P=0.003).결론 재CRS점막중표체상조적pendrin여MUCSAC적표체증가유일정관련,전자가능시CRS중점액고분비적중요인자.
Objective To explore the expression of pendrin in sinonasal mucosal tissue of patients with chronic rhinosinusitis (CRS) and examine its relationship with mucin5AC (MUCSAC).Methods Thirty mucosal tissue (located on anterior wall of maxillary sinus ostium and close to uncinate process) of patients with CRS with or without nasal polyps and 10 normal sinonasal mucosal tissue located at the same sites as CRS were collected.Histological changes of sinonasal mucosa were examined by hematoxylin and eosin (HE) staining.The expression of total mucins was evaluated by periodic acid Schiff staining (PAS).And the expressions of pendrin and MUC5AC in sinonasal mucosa were determined by immunohistochemistry (IHC),quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot.Results The tissue samples demonstrated mucosal thickening,goblet cell hyperplasia and metaplasia,epithelial cilia partial deletion,glandular hyperplasia and a dense infiltration of inflammatory cells in CRS with or without nasal polyps.IHC revealed that positive pendrin was expressed dominantly at apical membrane of epithelial cells and sparsely in submucosal glandular cells,there was a positive expression rate of CRS with nasal polyps and CRS without nasal polyps;MUCSAC was expressed abundantly in goblet cells and pseudostratified ciliated columnar epithelium cells and weakly in submucosal glandular cells in CRS.There were significant increases in positive expression rate of total mucins production,pendrin and MUC5AC in patients of CRS with nasal polyps or without nasal polyps than those in control (86.7％ and 80.0％ vs 20.0％,73.3％ and 70.0％ vs 10.0％,83.3％ and 76.7％ vs 20.0％,all P＜0.05);the relative expression levels of mRNA and protein expression of pendrin were significantly greater in patients of CRS with or without nasal polyps than those in controls (1.685 ± 0.111 and 1.537 ± 0.262 vs 1.012 ± 0.167,2.066 ± 0.188 and 1.996 ± 0.153 vs 1.000 ± 0.169,all P ＜ 0.05).The relative expression levels of mRNA expression of MUC5AC were significantly greater in patients of CRS with or without nasal polyps than in controls (1.861 ±0.718 and 1.562 ±0.428 vs 1.004 ± 0.094,P ＜ 0.05).And the expression level of pendrin mRNA was positively correlated with that of MUC5AC mRNA in patients with CRS (r =0.670,P =0.006 and r =0.713,P =0.003).Conclusions An up-regulation of pendrin is correlated with an over-expression of MUC5AC in patients with CRS.And pendrin is an important cause of mucus hypersecretion in CRS.
