中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
14期
1113-1115
,共3页
陈宜%李方烃%孟宪芬%黎晓新
陳宜%李方烴%孟憲芬%黎曉新
진의%리방경%맹헌분%려효신
槲皮素%视网膜新生血管%早产儿视网膜病变
槲皮素%視網膜新生血管%早產兒視網膜病變
곡피소%시망막신생혈관%조산인시망막병변
Quercetin%Retinal neovascularization%Retinopathy of prematurity
目的 探讨腹腔注射槲皮素抑制氧诱导视网膜病变(0IR)小鼠视网膜新生血管生成的作用及其可能机制.方法 将出生后7d的小鼠放入75％的氧气环境中5d,后在空气中恢复5d建立早产儿视网膜病变(ROP)的动物模型,并数字随机分成2组.A组(对照组,腹腔注射丙二醇,第12～17天);B组(槲皮素20 mg/kg治疗组,第12～17天).第17天处死小鼠,取出眼球固定,行视网膜铺片并ADP酶染色,光镜下评估视网膜新生血管的情况.苏木精-伊红染色法(HE染色)比较两组突破视网膜内界膜的血管内皮细胞核数.酶联免疫法(ELISA)分析血管内皮生长因子(VEGF)蛋白含量的变化.结果 治疗组新生血管发生率为82.1％,对照组新生血管发生率为100％(P＞0.1);治疗组新生血管的严重程度钟点数均值为6.3±2.8,对照组为9.0±2.2(P＜0.01).槲皮素治疗组突破视网膜内界膜的血管内皮细胞计数为(28.0±5.1)个,对照组为(47.0±4.2)个,差异有统计学意义(P＜0.05).槲皮素组视网膜中VEGF含量(118.2±6.2) pg/mg,为较对照组(146.3±4.3)pg/mg(P＜0.05),差异有统计学意义.结论 槲皮素可抑制视网膜新生血管的形成,可能作为治疗早产儿视网膜病变的新途径.
目的 探討腹腔註射槲皮素抑製氧誘導視網膜病變(0IR)小鼠視網膜新生血管生成的作用及其可能機製.方法 將齣生後7d的小鼠放入75％的氧氣環境中5d,後在空氣中恢複5d建立早產兒視網膜病變(ROP)的動物模型,併數字隨機分成2組.A組(對照組,腹腔註射丙二醇,第12～17天);B組(槲皮素20 mg/kg治療組,第12～17天).第17天處死小鼠,取齣眼毬固定,行視網膜鋪片併ADP酶染色,光鏡下評估視網膜新生血管的情況.囌木精-伊紅染色法(HE染色)比較兩組突破視網膜內界膜的血管內皮細胞覈數.酶聯免疫法(ELISA)分析血管內皮生長因子(VEGF)蛋白含量的變化.結果 治療組新生血管髮生率為82.1％,對照組新生血管髮生率為100％(P＞0.1);治療組新生血管的嚴重程度鐘點數均值為6.3±2.8,對照組為9.0±2.2(P＜0.01).槲皮素治療組突破視網膜內界膜的血管內皮細胞計數為(28.0±5.1)箇,對照組為(47.0±4.2)箇,差異有統計學意義(P＜0.05).槲皮素組視網膜中VEGF含量(118.2±6.2) pg/mg,為較對照組(146.3±4.3)pg/mg(P＜0.05),差異有統計學意義.結論 槲皮素可抑製視網膜新生血管的形成,可能作為治療早產兒視網膜病變的新途徑.
목적 탐토복강주사곡피소억제양유도시망막병변(0IR)소서시망막신생혈관생성적작용급기가능궤제.방법 장출생후7d적소서방입75％적양기배경중5d,후재공기중회복5d건립조산인시망막병변(ROP)적동물모형,병수자수궤분성2조.A조(대조조,복강주사병이순,제12～17천);B조(곡피소20 mg/kg치료조,제12～17천).제17천처사소서,취출안구고정,행시망막포편병ADP매염색,광경하평고시망막신생혈관적정황.소목정-이홍염색법(HE염색)비교량조돌파시망막내계막적혈관내피세포핵수.매련면역법(ELISA)분석혈관내피생장인자(VEGF)단백함량적변화.결과 치료조신생혈관발생솔위82.1％,대조조신생혈관발생솔위100％(P＞0.1);치료조신생혈관적엄중정도종점수균치위6.3±2.8,대조조위9.0±2.2(P＜0.01).곡피소치료조돌파시망막내계막적혈관내피세포계수위(28.0±5.1)개,대조조위(47.0±4.2)개,차이유통계학의의(P＜0.05).곡피소조시망막중VEGF함량(118.2±6.2) pg/mg,위교대조조(146.3±4.3)pg/mg(P＜0.05),차이유통계학의의.결론 곡피소가억제시망막신생혈관적형성,가능작위치료조산인시망막병변적신도경.
Objective To determine whether quercetin can suppress retinal angiogenesis in a rodent model of retinopathy of prematurity (ROP).Methods ROP was induced in C57BL/6 mice by exposing 7-day-old mice to 75％ oxygen (hyperoxia) for 5 days followed by 5 days in room air.Quercetin (20 mg/kg body weight) was administered intraperitoneally from Days 12-17.Control group received an intraperitoneal dose of propylene glycol.At Day 17,the eyes were enucleated and retinae diseected for ADPase and hematoxylin and eosin staining.The severity of neovsascularization were graded as the number of clock hour of new vessel (NV) with a masked approach.The proliferative neovascular response was quantifled by counting the nuclei of new vessels extending from retina into vitreous in cross-sections.And vascular endothelial growth factor (VEGF) was detected by enzyme-linked immunosorbent assay (ELISA).Results The severity of NV decreased significantly in Quercetin treatment group versus control group (P ＜ 0.05).The number of endothelial cells of new vessels extending from retina to viueous decreased significantly in quercetin treatment group versus hyperoxia group (P ＜ 0.05).The retinal level of VEGF decreased significantly in quercetin treatment group.Conclusion Quercetin attenuates retinal angiogenesis so that it may become a potential preventive chemotherapy for ROP.
