CAJ | 학술논문

通过比较红曲霉菌株在9种不同发酵基质中的色素产量，筛选最适合的发酵基质；在此基础上外加碳氮源，比较不同种类的碳氮源对红曲霉产色素能力的影响。研究结果表明：红曲霉菌株在以红米为基质的固态发酵体系中的色素产量最高，其醇溶性总色价为287．25 U／g，水溶性总色价为95．55 U／g；以红米为发酵基质，通过添加不同种类的碳源和氮源进行发酵，发现以果糖为外加碳源时红曲霉菌株的醇溶性色素产量可提高到859．87 U／g，色调偏黄；其次是葡萄糖，其醇溶性色价为812．17 U／g，其色调偏橙红；以氯化铵作为外加氮源时红曲霉菌株的醇溶性总色价高达1560．17 U／g，相比不外加任何氮源的对照组提高了6倍左右，其色调偏黄；其次是牛肉膏，醇溶性总色价为1179．07 U／g，色调偏红。
통과비교홍곡매균주재9충불동발효기질중적색소산량，사선최괄합적발효기질；재차기출상외가탄담원，비교불동충류적탄담원대홍곡매산색소능력적영향。연구결과표명：홍곡매균주재이홍미위기질적고태발효체계중적색소산량최고，기순용성총색개위287．25 U／g，수용성총색개위95．55 U／g；이홍미위발효기질，통과첨가불동충류적탄원화담원진행발효，발현이과당위외가탄원시홍곡매균주적순용성색소산량가제고도859．87 U／g，색조편황；기차시포도당，기순용성색개위812．17 U／g，기색조편등홍；이록화안작위외가담원시홍곡매균주적순용성총색개고체1560．17 U／g，상비불외가임하담원적대조조제고료6배좌우，기색조편황；기차시우육고，순용성총색개위1179．07 U／g，색조편홍。
By comparing the effects of nine different fermentation matrixes on the production of monascus pigment to screen the best fermentation matrix.And then,the effects of different kinds of additional carbon/nitrogen source on the production capacity of monascus pigment are researched.The results show that use red rice as fermentation matrix can get the highest pigment value:287.25 U/g of alcohol-soluble pigment and 95.55 U/g of water-soluble pigment.Therefore,red rice is selected as fermentation substrate by adding different kinds of carbon sources and nitrogen sources for fermentation,use fructose can increase the production of alcohol-soluble monascus pigment to 859.87 U/g,the color hue is partial to yellow.The second highest yield is glucose,whose alcohol-soluble color value is 812.17 U/g,but the color hue is partial to orange.Use NH4 Cl as additional nitrogen source can increase the alcohol-soluble color value to 1560.17 U/g,about 6 times higher than that of no nitrogen source control group,whose color hue is partial to yellow,followed by beef extract, whose alcohol-soluble color value is 1179.07 U/g,and the color hue is partial to red.