CAJ | 학술논문

目的：寻找与英夫利西单抗（infliximab，IFX）治疗克罗恩病（Crohn’s disease，CD）黏膜愈合（muco-sal healing，MH）相关的血清蛋白质生物标志物。方法：采集7例经IFX治疗获得MH的CD患者治疗前（0周，A组）和治疗后（14周，B组）的血清，以及7例未获得MH的CD患者（0周为C组，14周为D组）的血清。采用荧光标记双向差异凝胶电泳的方法，比较A组与B组之间、C组与D组之间、A组与C组之间以及B组与D组之间的蛋白质组学差异，并对差异表达的蛋白质斑点进行基质辅助激光解吸／电离飞行时间串联质谱初步鉴定和生物信息学分析。结果：（1）A组与B组、C组与D组、A组与C组以及B组与D组之间比较分别存在36、3、10和31个差异表达的蛋白质斑点，共计存在44个显著差异表达的蛋白质斑点。（2）上述各组之间的差异斑点分别初步鉴定出17、2、2和15种蛋白质，共计存在19种差异表达的蛋白质，包括载脂蛋白E、载脂蛋白A-I、补体因子H等。（3）基于STRING数据库绘制了蛋白质网状功能图。结论：IFX治疗获得MH的CD患者治疗前后的血清蛋白质表达谱存在差异，获得MH和未获得MH的患者血清表达谱亦存在差异，其中19种蛋白质有可能成为预测CD患者使用IFX获得MH的生物标志物。
목적：심조여영부리서단항（infliximab，IFX）치료극라은병（Crohn’s disease，CD）점막유합（muco-sal healing，MH）상관적혈청단백질생물표지물。방법：채집7례경IFX치료획득MH적CD환자치료전（0주，A조）화치료후（14주，B조）적혈청，이급7례미획득MH적CD환자（0주위C조，14주위D조）적혈청。채용형광표기쌍향차이응효전영적방법，비교A조여B조지간、C조여D조지간、A조여C조지간이급B조여D조지간적단백질조학차이，병대차이표체적단백질반점진행기질보조격광해흡／전리비행시간천련질보초보감정화생물신식학분석。결과：（1）A조여B조、C조여D조、A조여C조이급B조여D조지간비교분별존재36、3、10화31개차이표체적단백질반점，공계존재44개현저차이표체적단백질반점。（2）상술각조지간적차이반점분별초보감정출17、2、2화15충단백질，공계존재19충차이표체적단백질，포괄재지단백E、재지단백A-I、보체인자H등。（3）기우STRING수거고회제료단백질망상공능도。결론：IFX치료획득MH적CD환자치료전후적혈청단백질표체보존재차이，획득MH화미획득MH적환자혈청표체보역존재차이，기중19충단백질유가능성위예측CD환자사용IFX획득MH적생물표지물。
AIM:To identify the serum proteins that might serve as biomarkers for predicting mucosal healing ( MH) in the patients with Crohn’ s disease ( CD) treated with infliximab ( IFX) .METHODS:We collected serum sam-ples before treatment (0 week, group A) and 14 weeks after treatment (group B) from 7 CD patients with IFX treatment who had achieved MH, as well as the serum samples from 7 CD patients who had not achieved MH (0 week, group C;14 weeks, group D) .Two-dimensional fluorescence difference gel electrophoresis was applied to analyze and compare the re-sults of serum profiles between groups A and B, C and D, A and C, B and D.Matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry and bioinformatics tools were utilized to preliminarily identify and figure out the dif-ferentially expressed proteins.RESULTS:(1) In total, there were 44 differentially expressed spots, 36, 3, 10 and 31 differentially expressed spots were detected while comparing A with B, C with D, A with C and B with D, respectively. (2) Among those spots, 17, 2, 2 and 15 proteins were identified, respectively.In total, there were 19 differentially ex-pressed proteins, including apolipoprotein E, apolipoprotein A-I, complement factor H, and so on.(3) Protein functional association networks were carried out based on STRING database.CONCLUSION: The serum protein profiles obviously change after IFX treatment in MH CD patients, and the serum protein profiles of MH patients are different from that of non-MH patients after IFX treatment.The 19 proteins we identified may serve as potential biomarkers for predicting MH in CD patients with IFX treatment.