CAJ | 학술논문

目的：建立测定去甲斑蝥素和齐墩果酸复合脂质体包封率的反相高效液相色谱（RP-HPLC）法。方法色谱柱为Thermo Sycronis C18柱(150 mm ×4.6 mm，5μm )，梯度洗脱，检测波长为210 nm，柱温为30℃。超速离心法分离脂质体中游离药物。结果去甲斑蝥素质量浓度在20~300μg/mL范围内与峰面积线性关系良好，r=0.9998，平均回收率为99.15%~100.11%，RSD≤3.20%（ n=5）；齐墩果酸质量浓度在10~150μg/mL范围内与峰面积线性关系良好，r=0.9997，平均回收率为99.22%~100.30%，RSD≤3.34%（ n=5）。结论该方法简便、快速，可用于去甲斑蝥素齐墩果酸复合脂质体包封率的测定。
목적：건립측정거갑반모소화제돈과산복합지질체포봉솔적반상고효액상색보（RP-HPLC）법。방법색보주위Thermo Sycronis C18주(150 mm ×4.6 mm，5μm )，제도세탈，검측파장위210 nm，주온위30℃。초속리심법분리지질체중유리약물。결과거갑반모소질량농도재20~300μg/mL범위내여봉면적선성관계량호，r=0.9998，평균회수솔위99.15%~100.11%，RSD≤3.20%（ n=5）；제돈과산질량농도재10~150μg/mL범위내여봉면적선성관계량호，r=0.9997，평균회수솔위99.22%~100.30%，RSD≤3.34%（ n=5）。결론해방법간편、쾌속，가용우거갑반모소제돈과산복합지질체포봉솔적측정。
Objective To establish a reversed-phase high performance liquid chromatography(RP-HPLC) method for the determination of entrapment efficiency of norcantharidin and oleanolic acid complex liposome. Methods The separation was achieved by using the Thermo Sycronis C18 column ( 150 mm × 4. 6 mm,5 μm ) with the gradient elution. The detection wavelength was set at 210 nm and the column temperature was 30 ℃. The free drugs in liposome were separated by using the upercentrifugation. Results The mass concentra-tion of norcantharidin in the range of 20-300 μg/mL showed the good linear relation with the peak area and the average recovery was 99. 15% -100. 11%,RSD ≤ 3. 20%( n=5);the mass concentration of oleanolic acid in the range of 10-150 μg/mL showed the good linear relation with the peak area and the average recovery was 99. 22% -100. 30%,RSD≤3. 34%( n=5). Conclusion The method is simple,fast,and thus can be applied to the determination of entrapment efficiency of norcantharidin and oleanolic acid com-plex liposome.