山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2015年
18期
30-33
,共4页
汪泽%高艳艳%刘永全%高福生
汪澤%高豔豔%劉永全%高福生
왕택%고염염%류영전%고복생
支气管哮喘%烟曲霉%气道高反应性%气道炎症%气道重构%大鼠
支氣管哮喘%煙麯黴%氣道高反應性%氣道炎癥%氣道重構%大鼠
지기관효천%연곡매%기도고반응성%기도염증%기도중구%대서
bronchial asthma%Aspergillus fumigates%bronchial hyperresponsiveness%airway inflammation%airwayremodeling%rats
目的：探讨慢性烟曲霉暴露对支气管哮喘大鼠气道高反应性、气道炎症和气道重构的影响。方法将56只雄性Wistar大鼠随机分为A组（慢性哮喘组）、B组（慢性哮喘＋烟曲霉孢子滴入组）、C组（慢性哮喘＋生理盐水滴入组）、D组[卵白蛋白（ OVA）致敏＋生理盐水激发＋生理盐水滴入组]、E组（ OVA致敏＋生理盐水激发＋烟曲霉孢子滴入组），B组再分成B1、B2、B3组（分别吸入烟曲霉1、3、5周），每组各8只。测定大鼠气道阻力（Raw）及乙酰胆碱注射后Raw变化率；ELISA法测定血清中总IgE和肺泡灌洗液（ BALF）中IL-13、IL-5、IFN-γ、TGF-β水平；制作肺组织切片，并行HE染色、过碘酸雪肤（ PAS）染色、Masson三色染色和病理图像形态学测定，观察各组气道上皮细胞损伤脱落、杯状细胞增生及上皮下纤维化程度。结果 B3组Raw和BALF中IL-5、IL-13、TGF-β水平及血清总IgE水平、炎细胞浸润、嗜酸性粒细胞浸、脱落上皮长度／气道上皮内径、杯状细胞增生、上皮下胶原纤维沉积均高于A、C、D、E组（P＜0．05或P＜0，01）。结论慢性烟曲霉暴露可加重支气管哮喘大鼠气道高反应性、气道炎症和气道重构程度。
目的：探討慢性煙麯黴暴露對支氣管哮喘大鼠氣道高反應性、氣道炎癥和氣道重構的影響。方法將56隻雄性Wistar大鼠隨機分為A組（慢性哮喘組）、B組（慢性哮喘＋煙麯黴孢子滴入組）、C組（慢性哮喘＋生理鹽水滴入組）、D組[卵白蛋白（ OVA）緻敏＋生理鹽水激髮＋生理鹽水滴入組]、E組（ OVA緻敏＋生理鹽水激髮＋煙麯黴孢子滴入組），B組再分成B1、B2、B3組（分彆吸入煙麯黴1、3、5週），每組各8隻。測定大鼠氣道阻力（Raw）及乙酰膽堿註射後Raw變化率；ELISA法測定血清中總IgE和肺泡灌洗液（ BALF）中IL-13、IL-5、IFN-γ、TGF-β水平；製作肺組織切片，併行HE染色、過碘痠雪膚（ PAS）染色、Masson三色染色和病理圖像形態學測定，觀察各組氣道上皮細胞損傷脫落、杯狀細胞增生及上皮下纖維化程度。結果 B3組Raw和BALF中IL-5、IL-13、TGF-β水平及血清總IgE水平、炎細胞浸潤、嗜痠性粒細胞浸、脫落上皮長度／氣道上皮內徑、杯狀細胞增生、上皮下膠原纖維沉積均高于A、C、D、E組（P＜0．05或P＜0，01）。結論慢性煙麯黴暴露可加重支氣管哮喘大鼠氣道高反應性、氣道炎癥和氣道重構程度。
목적：탐토만성연곡매폭로대지기관효천대서기도고반응성、기도염증화기도중구적영향。방법장56지웅성Wistar대서수궤분위A조（만성효천조）、B조（만성효천＋연곡매포자적입조）、C조（만성효천＋생리염수적입조）、D조[란백단백（ OVA）치민＋생리염수격발＋생리염수적입조]、E조（ OVA치민＋생리염수격발＋연곡매포자적입조），B조재분성B1、B2、B3조（분별흡입연곡매1、3、5주），매조각8지。측정대서기도조력（Raw）급을선담감주사후Raw변화솔；ELISA법측정혈청중총IgE화폐포관세액（ BALF）중IL-13、IL-5、IFN-γ、TGF-β수평；제작폐조직절편，병행HE염색、과전산설부（ PAS）염색、Masson삼색염색화병리도상형태학측정，관찰각조기도상피세포손상탈락、배상세포증생급상피하섬유화정도。결과 B3조Raw화BALF중IL-5、IL-13、TGF-β수평급혈청총IgE수평、염세포침윤、기산성립세포침、탈락상피장도／기도상피내경、배상세포증생、상피하효원섬유침적균고우A、C、D、E조（P＜0．05혹P＜0，01）。결론만성연곡매폭로가가중지기관효천대서기도고반응성、기도염증화기도중구정도。
Objective To investigate the effects of chronic Aspergillus fumigatus ( A.fumigatus ) exposure on the bronchial hyperresponsiveness , airway inflammation and remodeling in rats with bronchial asthma .Methods Sixty-four male Wistar rats were randomly divided into group A ( chronic asthma group ) , group B ( chronic asthma +Af spore inha-lation group), group C (chronic asthma +saline inhalation group), group D (OVA-sensitized and saline-challenged +saline inhalation group ) and group E ( OVA-sensitized and saline-challenged +Af spore inhalation group ) .Meanwhile , group B was then divided into groups B1, B2 and B3 (Af spore inhalation for 1 week, 3 weeks and 5 weeks) (n=8).The airway resistance ( Raw ) and change rate of R aw after administration of acetylcholine chloride were measured by using a com-puterized system.The concentration of total serum IgE and IL-5, IL-13, IFN-γ, TGF-βlevels in the bronchoalveolar lav-age fluid ( BALF) were measured by ELISA .Lung sections were made and then received hematoxylin and eosin ( HE) stai-ning, periodic acid Schiff ( PAS) staining and Masson trichrome staining .The images were analyzed morphometrically to e-valuate the airway epithelial detachment , goblet cell hyperplasia and subepithelial fibrosis in rats of the 7 groups .Results The change rate of R aw after administration of acetylcholine chloride , the IL-5, IL-13, TGF-βlevels, the concentration of total serum IgE, the inflammatory cell infiltration, the eosinophils, the airway epithelial detachment length/epithelial diam-eter, the goblet cell hyperplasia and subepithelial collagen deposition were all higher than those in the groups of A , C, D and E(P<0.05 or P<0.01).Conclusions Chronic Aspergillus fumigatus (A.fumigatus) exposure aggravates the bronchial hyperresponsiveness , airway inflammation and the degree of airway remodeling in rats with bronchial asthma .
