CAJ | 학술논문

目的：观察缺血预处理（ IPC）对下颌下腺缺血再灌注（ I／R）损伤大鼠的保护作用，并探讨其机制。方法将48只大鼠随机分为模型组和观察组各24只，均制作右侧下颌下腺I／R损伤模型。观察组在制模前行IPC，夹闭颌外动脉下颌下腺供血支3 min，再灌注3 min，重复3次。以左侧下颌下腺作为对照组。再灌注1、12、24、72 h，取6只大鼠检测各组腺体分泌量、组织学变化、紧密连接结构及M受体表达情况。结果对照组腺体分泌量、组织形态、紧密连接结构及M受体表达情况均正常。 I／R后1、12 h，模型组腺体分泌量明显降低，观察组稍有降低，24 h时两组分泌量开始增加，至72 h逐渐恢复正常。模型组腺实质细胞萎缩，间质水肿及炎症浸润，电镜下超微结构观察显示紧密连接结构破坏，宽度降低，均在再灌注12 h后最为严重，观察组部分腺体组织出现上述情况，且反应较轻。模型组I／R后，M受体水平1、12 h下降明显，观察组稍有下降，72 h时逐渐恢复。结论 IPC对下颌下腺I／R损伤大鼠具有保护作用，其机制与上调下颌下腺M受体的表达、保护下颌下腺紧密连接结构有关。
목적：관찰결혈예처리（ IPC）대하합하선결혈재관주（ I／R）손상대서적보호작용，병탐토기궤제。방법장48지대서수궤분위모형조화관찰조각24지，균제작우측하합하선I／R손상모형。관찰조재제모전행IPC，협폐합외동맥하합하선공혈지3 min，재관주3 min，중복3차。이좌측하합하선작위대조조。재관주1、12、24、72 h，취6지대서검측각조선체분비량、조직학변화、긴밀련접결구급M수체표체정황。결과대조조선체분비량、조직형태、긴밀련접결구급M수체표체정황균정상。 I／R후1、12 h，모형조선체분비량명현강저，관찰조초유강저，24 h시량조분비량개시증가，지72 h축점회복정상。모형조선실질세포위축，간질수종급염증침윤，전경하초미결구관찰현시긴밀련접결구파배，관도강저，균재재관주12 h후최위엄중，관찰조부분선체조직출현상술정황，차반응교경。모형조I／R후，M수체수평1、12 h하강명현，관찰조초유하강，72 h시축점회복。결론 IPC대하합하선I／R손상대서구유보호작용，기궤제여상조하합하선M수체적표체、보호하합하선긴밀련접결구유관。
Objective To observe the protective effect of ischemic preconditioning ( IPC) on the rat submandibular gland after ischemia-reperfusion ( I/R) injury and to investigate the mechanism .Methods Forty-eight healthy male Wist-ar rats were randomly divided into the observation group and model group , 24 in each group .The right glands of rats in both groups received the I/R injury to make the I/R injury models .The glands of the observation group were obtained by 3 min of ischemia , followed by 3 min of reperfusion , and we performed three times before modeling .The left glands were taken as the control group .Salivary secretion , histological changes , alternations of tight junctions ( TJs) and mACh-recep-tor levels in 6 rats were detected at different time points (1 h, 12 h, 24 h and 72 h after I/R).Results In the control group, the salivary secretion , histological changes , alternations of tight junctions ( TJs) and mACh-receptor expression lev-els were all normal .In the model group , the secretion of the glands significantly decreased after I /R 1 h and 12 h, and it showed a slightly lower in the observation group .The secretion of both groups gradually increased at 24 h and got back to normal at 72 h after reperfusion .Cellular atrophy , inflammatory cells infiltration , tissue edema , the collapse and reduced widths of TJs were observed especially after reperfusion for 12 h in the model group , a little part showed the status and the reaction was lighter in the observation group .The level of mACh-receptor exhibited the same tendency , and lower mACh-receptor levels at 12 h after reperfusion were observed in the model group;while in the observation group , it gradually re-turned to normal.Conclusion IPC has the protective effect on the rat submandibular gland after I /R injury, whose mech-anism may be related with the down-regulation of mACh-receptor and protection of TJs .