CAJ | 학술논문

目的：探讨2-羟丙基-β-环糊精（2HP-β-CD）对人脐静脉内皮细胞（HUVEC）体外增殖和迁移的作用。方法体外培养HUVEC，分为两组，实验组分别加入浓度为10－10、10－9、10－8、10－7、10－6、10－5 mol／L的2HP-β-CD，空白组加入含0．5％FBS的等量培养液。采用CCK-8法检测各组细胞增殖活力，改良Boyden Chamber法检测各组细胞的迁移能力。结果实验组中，加入10－10、10－9、10－8 mol／L 2HP-β-CD者的OD450分别为空白组的1．4、1．6、1．7倍，10－8 mol／L 2 HP-β-CD增强细胞增殖活力的作用最明显（ P均＜0．01）；加入10－7、10－6、10－5 mol／L 2 HP-β-CD者的OD450与空白组比较差异无统计学意义。加入10－9及10－8 mol／L 2HP-β-CD者细胞迁移数显著高于空白组（P均＜0．05），其他浓度组细胞迁移数与空白组比较差异均无统计学意义。结论适当浓度的2HP-β-CD可促进HUVEC的增殖和迁移。
목적：탐토2-간병기-β-배호정（2HP-β-CD）대인제정맥내피세포（HUVEC）체외증식화천이적작용。방법체외배양HUVEC，분위량조，실험조분별가입농도위10－10、10－9、10－8、10－7、10－6、10－5 mol／L적2HP-β-CD，공백조가입함0．5％FBS적등량배양액。채용CCK-8법검측각조세포증식활력，개량Boyden Chamber법검측각조세포적천이능력。결과실험조중，가입10－10、10－9、10－8 mol／L 2HP-β-CD자적OD450분별위공백조적1．4、1．6、1．7배，10－8 mol／L 2 HP-β-CD증강세포증식활력적작용최명현（ P균＜0．01）；가입10－7、10－6、10－5 mol／L 2 HP-β-CD자적OD450여공백조비교차이무통계학의의。가입10－9급10－8 mol／L 2HP-β-CD자세포천이수현저고우공백조（P균＜0．05），기타농도조세포천이수여공백조비교차이균무통계학의의。결론괄당농도적2HP-β-CD가촉진HUVEC적증식화천이。
Objective To investigate the effect of 2-hydroxypropyl-β-cyclodextrin (2HP-β-CD) on the migration and proliferation of human umbilical vein endothelial cells ( HUVECs) in vitro.Methods HUVECs were cultured in vitro and were divided into two groups:the experimental group and the blank group .HUVECs in the experimental group were treated with different concentrations of 2HP-β-CD (10 -10-10 -5 mol/L), and the same amount of 0.5% FBS was added into the blank group .CCK-8 was used to detect the cell proliferation activities of the two groups , and the modified Boyden Chamber was applied to observe the cell migration in each group .Results The OD450 of the experimental group which was added with 10 -10 mol/L, 10 -9 mol/L and 10 -8 mol/L 2HP-β-CD was 1.4, 1.6 and 1.7 times higher than that of the blank group;and 10 -10 mol/L 2HP-β-CD had the most significant effect in promoting the proliferation of HUVECs ( all P <0.01).No significant difference was found in OD450 of the two groups when we added 10 -7, 10 -6 and 10 -5 mol/L 2HP-β-CD to HUVECs.The migration of HUVECs which were added with 2HP-β-CD at the concentration of 10 -9 mol/L and 10 -8 mol/L in the experimental group was significantly higher than that of the blank group (all P<0.05).When we added other concentrations of 2HP-β-CD, no significant difference was found in the cell migration between the experimental and blank groups.Conclusion Suitable concentrations of 2HP-β-CD can promote the migration and proliferation of HUVECs .