CAJ | 학술논문

病毒诱导基因沉默(virus-induced genetic silencing，VIGS)是携带目的基因片段的病毒侵染植物后，诱导植物体内相同序列基因不表达的现象，它是一种转录后水平的基因沉默(post-transcriptional gene silencing，PTGS)现象。近年来，在烟草基因功能探究和抗病育种的研究中VIGS获得了广泛的应用。阐述了RNA沉默的发现和作用机制，VIGS在烟草基因功能鉴定上的发现和烟草育种上的进展，探讨了VIGS技术在烟草中影响沉默效率的因素、局限性和发展前景。
병독유도기인침묵(virus-induced genetic silencing，VIGS)시휴대목적기인편단적병독침염식물후，유도식물체내상동서렬기인불표체적현상，타시일충전록후수평적기인침묵(post-transcriptional gene silencing，PTGS)현상。근년래，재연초기인공능탐구화항병육충적연구중VIGS획득료엄범적응용。천술료RNA침묵적발현화작용궤제，VIGS재연초기인공능감정상적발현화연초육충상적진전，탐토료VIGS기술재연초중영향침묵효솔적인소、국한성화발전전경。
Virus-induced genetic silencing is the silent phenomenon of plant inner identical sequence gene which is induced by the virus disseminated plant of carried target gene frame.It is a post-transcriptional gene silencing phenomenon(PTGS).Recently,VIGS technology was used widely in the exploration of tobacco gene function and the research of breeding disease resistance.This review introduced the discovery of RNA silencing and mechanism of action,and described the VIGS technology in the discovery of tobacco gene function and the progress of breeding tobacco. Additionally,the factors that influenced efficiency of silencing,limitation and development prospect of VIGS were studied.