现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2015年
8期
9-12
,共4页
龙卫%艾辛%蒋建雄%朱玉叶%李莎莎%支添添
龍衛%艾辛%蔣建雄%硃玉葉%李莎莎%支添添
룡위%애신%장건웅%주옥협%리사사%지첨첨
荻%光周期%Hd3a%FT
荻%光週期%Hd3a%FT
적%광주기%Hd3a%FT
Miscanthus Sacchariflorus%photoperiod%Hd3a%FT
FT(Flowering locus T)基因是最初在拟南芥中发现的一个控制光周期途径开花的一个关键基因,禾本科水稻中发现Hd3a(Heading date 3a)基因被证明与FT的同源且功能相同。该研究采用同源序列法通过水稻Hd3a的mRNA序列在近缘物种甜高粱基因组序列中进行BLAST得到cDNA序列进行引物设计,从禾本科芒属荻中克隆出2条FT的同源基因,命名为MsFT1和MsFT2。MsFT1全长543 bp,编码181个氨基酸;MsFT2全长540 bp,编码180个氨基酸。MsFT1和MsFT2的碱基序列相似性达98%,推导的氨基酸序列相似性达97%,均含有PEBP特异结构域。通过clustal软件将MsFT1和MsFT2与其他物种FT基因比较显示与甜高粱的相似性最高达98%、96%,其次是玉米、小麦、水稻等禾本科植物相似度较高。将MsFT1和MsFT2氨基酸序列和水稻FT家族18个成员进行对比,MsFT1及MsFT2属于FT家族中的FT-like亚族,且与Hd3a处于同一进化分支上。
FT(Flowering locus T)基因是最初在擬南芥中髮現的一箇控製光週期途徑開花的一箇關鍵基因,禾本科水稻中髮現Hd3a(Heading date 3a)基因被證明與FT的同源且功能相同。該研究採用同源序列法通過水稻Hd3a的mRNA序列在近緣物種甜高粱基因組序列中進行BLAST得到cDNA序列進行引物設計,從禾本科芒屬荻中剋隆齣2條FT的同源基因,命名為MsFT1和MsFT2。MsFT1全長543 bp,編碼181箇氨基痠;MsFT2全長540 bp,編碼180箇氨基痠。MsFT1和MsFT2的堿基序列相似性達98%,推導的氨基痠序列相似性達97%,均含有PEBP特異結構域。通過clustal軟件將MsFT1和MsFT2與其他物種FT基因比較顯示與甜高粱的相似性最高達98%、96%,其次是玉米、小麥、水稻等禾本科植物相似度較高。將MsFT1和MsFT2氨基痠序列和水稻FT傢族18箇成員進行對比,MsFT1及MsFT2屬于FT傢族中的FT-like亞族,且與Hd3a處于同一進化分支上。
FT(Flowering locus T)기인시최초재의남개중발현적일개공제광주기도경개화적일개관건기인,화본과수도중발현Hd3a(Heading date 3a)기인피증명여FT적동원차공능상동。해연구채용동원서렬법통과수도Hd3a적mRNA서렬재근연물충첨고량기인조서렬중진행BLAST득도cDNA서렬진행인물설계,종화본과망속적중극륭출2조FT적동원기인,명명위MsFT1화MsFT2。MsFT1전장543 bp,편마181개안기산;MsFT2전장540 bp,편마180개안기산。MsFT1화MsFT2적감기서렬상사성체98%,추도적안기산서렬상사성체97%,균함유PEBP특이결구역。통과clustal연건장MsFT1화MsFT2여기타물충FT기인비교현시여첨고량적상사성최고체98%、96%,기차시옥미、소맥、수도등화본과식물상사도교고。장MsFT1화MsFT2안기산서렬화수도FT가족18개성원진행대비,MsFT1급MsFT2속우FT가족중적FT-like아족,차여Hd3a처우동일진화분지상。
FT(Flowering locus T)gene was originally discovered in Arabidopsis as a photoperiodic control of flowering,It’s a key pathway genes. Hd3a(Heading date 3a)which was found in rice grassy was proven to be a homologous genes of FT and had the same feature.In this study,we designed primer by homologous cDNA sequence in sorghum.Two homologous genes of FT were cloned in the species of Miscanthus sacchariflorus,named them MsFT1 and MsFT2.MsFT1 had full length of 543bp,encoding 181 amino acids;MsFT2 had full length of 540 bp,encoding 180 amino acids.The sequence of MsFT1 and MsF2 were of 98%the same,and they both had the spatial structure of PEBP.When MsFT1 and MsFT2 were compared with other species’ FT gene,They had more than 98%,96%similarities with sorghum,followed by corn,wheat,rice and other grasses.When comparing MsFT1 and MsF2 with rice FT/TFL1 family,MsFT1 and MsFT2 both belong to FT-like subfamily,and were at the same evolution branches with Hd3a.