医学检验与临床
醫學檢驗與臨床
의학검험여림상
MEDICAL LATORATORY SCIENCE AND CLINICES
2015年
2期
51-53
,共3页
Raji细胞%IL-12%肿瘤免疫%增殖%凋亡
Raji細胞%IL-12%腫瘤免疫%增殖%凋亡
Raji세포%IL-12%종류면역%증식%조망
Ragi cell%Interlekin 12%Tumor immunity%Proliferation%Apoptosis
目的：通过观察IL-12重组腺病毒载体对细胞毒性T淋巴细胞杀伤淋巴瘤Raji细胞增殖、凋亡的影响，为进一步基因治疗人Burkitt淋巴瘤提供实验依据。方法：培养淋巴瘤特异性CTL细胞，观察其对重组腺病毒载体转染Raji细胞的杀伤作用，MTT法检测Raji细胞的凋亡率，LDH释放实验检测CTL细胞毒作用。结果：各组均能抑制Raji细胞的生长。其中A组（Ad IL-12-Raji细胞与CTL细胞混合培养组）细胞凋亡率（49.1±7.2）%与CTL细胞的杀伤率（93.52±3.4）%均明显高于B、C两组。结论：经IL-12基因转染修饰的肿瘤细胞，可以增强CTL细胞的杀伤能力。
目的：通過觀察IL-12重組腺病毒載體對細胞毒性T淋巴細胞殺傷淋巴瘤Raji細胞增殖、凋亡的影響，為進一步基因治療人Burkitt淋巴瘤提供實驗依據。方法：培養淋巴瘤特異性CTL細胞，觀察其對重組腺病毒載體轉染Raji細胞的殺傷作用，MTT法檢測Raji細胞的凋亡率，LDH釋放實驗檢測CTL細胞毒作用。結果：各組均能抑製Raji細胞的生長。其中A組（Ad IL-12-Raji細胞與CTL細胞混閤培養組）細胞凋亡率（49.1±7.2）%與CTL細胞的殺傷率（93.52±3.4）%均明顯高于B、C兩組。結論：經IL-12基因轉染脩飾的腫瘤細胞，可以增彊CTL細胞的殺傷能力。
목적：통과관찰IL-12중조선병독재체대세포독성T림파세포살상림파류Raji세포증식、조망적영향，위진일보기인치료인Burkitt림파류제공실험의거。방법：배양림파류특이성CTL세포，관찰기대중조선병독재체전염Raji세포적살상작용，MTT법검측Raji세포적조망솔，LDH석방실험검측CTL세포독작용。결과：각조균능억제Raji세포적생장。기중A조（Ad IL-12-Raji세포여CTL세포혼합배양조）세포조망솔（49.1±7.2）%여CTL세포적살상솔（93.52±3.4）%균명현고우B、C량조。결론：경IL-12기인전염수식적종류세포，가이증강CTL세포적살상능력。
Objective:In order to understand the resistance mechanism and further overcome the Burkitt Lymphoma Raji Cells, we study the proliferation and apoptosis effect of adenovirus-mediated interleukin-12 on the Raji Cells. Methods:High ratio of activated CTL,which can kill Raji cells were gotten by DC. Cultered Raji Cells were infected with the recombinant adenovirus vector haboring IL-12 gene to establish Ad-IL12-Raji. The proliferation and apoptosis of Raji Cells were detected by MTT . The cytotoxicity of CTL were detected by lactate dehydrogenase(LDH).Results:The proliferation and apoptosis of Raji cell can be restrained in all groups. The proliferation and apoptosis(49.1±7.2)%,cytotoxicity of CTL(93.52±3.4)% in groups A can be significantly up-ragulated than the others. Conclusions:The cells with IL-12 gene transduction can inhibit the proliferation and induce apoptosis of Raji by induce the cytotoxicity of CTL.
