医学检验与临床
醫學檢驗與臨床
의학검험여림상
MEDICAL LATORATORY SCIENCE AND CLINICES
2015年
2期
13-14
,共2页
梅毒%血清学%检测方法%应用评价
梅毒%血清學%檢測方法%應用評價
매독%혈청학%검측방법%응용평개
Syphilis%serology%Detection method%Application evaluation
目的：探讨梅毒血清学不同检测方法在临床中的应用评价。方法：对我院2012年1月-12月964份住院和门诊标本同时用梅毒酶联免疫吸附试验(ELISA)和甲苯胺红不加热血清试验(TRUST)检测，上述任一方法阳性再进行梅毒螺旋体明胶凝集试验(TPPA)检测。结果：ELISA法检出61份阳性，TRUST法检出32份阳性，TPPA法复检出60份阳性，与EL1SA法比较，TRUST法符合率为52.46%，TPPA法符合率98.36%；与TPPA法比较， TRUST法符合率53.33%；TRUST法与ELISA法和TPPA法检出率有显著差异。结论：由于梅毒抗体检测方法的灵敏度，特异性，试验条件等不同，选择特异性抗体和非特异性抗体联合检测，测定梅毒不同期的抗体及其效价，有助于梅毒的临床诊断和病程观察。
目的：探討梅毒血清學不同檢測方法在臨床中的應用評價。方法：對我院2012年1月-12月964份住院和門診標本同時用梅毒酶聯免疫吸附試驗(ELISA)和甲苯胺紅不加熱血清試驗(TRUST)檢測，上述任一方法暘性再進行梅毒螺鏇體明膠凝集試驗(TPPA)檢測。結果：ELISA法檢齣61份暘性，TRUST法檢齣32份暘性，TPPA法複檢齣60份暘性，與EL1SA法比較，TRUST法符閤率為52.46%，TPPA法符閤率98.36%；與TPPA法比較， TRUST法符閤率53.33%；TRUST法與ELISA法和TPPA法檢齣率有顯著差異。結論：由于梅毒抗體檢測方法的靈敏度，特異性，試驗條件等不同，選擇特異性抗體和非特異性抗體聯閤檢測，測定梅毒不同期的抗體及其效價，有助于梅毒的臨床診斷和病程觀察。
목적：탐토매독혈청학불동검측방법재림상중적응용평개。방법：대아원2012년1월-12월964빈주원화문진표본동시용매독매련면역흡부시험(ELISA)화갑분알홍불가열혈청시험(TRUST)검측，상술임일방법양성재진행매독라선체명효응집시험(TPPA)검측。결과：ELISA법검출61빈양성，TRUST법검출32빈양성，TPPA법복검출60빈양성，여EL1SA법비교，TRUST법부합솔위52.46%，TPPA법부합솔98.36%；여TPPA법비교， TRUST법부합솔53.33%；TRUST법여ELISA법화TPPA법검출솔유현저차이。결론：유우매독항체검측방법적령민도，특이성，시험조건등불동，선택특이성항체화비특이성항체연합검측，측정매독불동기적항체급기효개，유조우매독적림상진단화병정관찰。
Objective:To explore different serological detection methods of syphilis in diagnosis and treatment of syphilis in clinical application evaluation. Methods:From January to December in 2012,964 samples collected from outpatients and inpatients were tested by Enzyme Linked Immunosorben Assay(ELISA)and Toluidine Red Unheated Serum Test(TRUST).Then the positive samples were tested by Treponema Pallidum Particle Assay (TPPA).Results:61 positive samples were detected by ELISA method. 32 positive samples were detected by TRUST method.60 positive samples were detected by TPPA retest. Comparing to ELISA method, the coincidence rate of TRUST was 52.46%, and the coincidence rate of TPPA was 98.36. Comparing to TPPA method, the coincidence rate of TRUST was 53.33%.The detection rate between TRUST and ELISA/TPPA was significantly different. Conclusions:Due to the sensitivity, specificity and condition of each antibodies detection method are diffident, in clinical diagnosis and disease course observation of syphilis, it helps to detect syphilis antibodies and titer in different stages combining the detection of both specific and nonspecific antibodies.
