中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2015年
9期
51-52
,共2页
高效液相色谱法%止咳定喘丸%苦杏仁苷%含量测定
高效液相色譜法%止咳定喘汍%苦杏仁苷%含量測定
고효액상색보법%지해정천환%고행인감%함량측정
HPLC%Zhike Dingchuan Pills%amygdalin%content determination
目的：建立测定止咳定喘丸中苦杏仁苷含量的高效液相色谱法。方法色谱柱为 Agela Venusil MP C18柱（250 mm ×4.6 mm，5μm），流动相为甲醇－水（20：80），流速为1.0 mL/min，检测波长为210 nm，柱温室温。结果苦杏仁苷进样量在0.0530~0.8480μg （ r=0.9999）范围内与峰面积积分值具有良好线性关系，平均加样回收率为98.64%，RSD为1.11%（ n=6）。结论该法简便、灵敏、准确可靠，可用于止咳定喘丸中苦杏仁苷的含量测定。
目的：建立測定止咳定喘汍中苦杏仁苷含量的高效液相色譜法。方法色譜柱為 Agela Venusil MP C18柱（250 mm ×4.6 mm，5μm），流動相為甲醇－水（20：80），流速為1.0 mL/min，檢測波長為210 nm，柱溫室溫。結果苦杏仁苷進樣量在0.0530~0.8480μg （ r=0.9999）範圍內與峰麵積積分值具有良好線性關繫，平均加樣迴收率為98.64%，RSD為1.11%（ n=6）。結論該法簡便、靈敏、準確可靠，可用于止咳定喘汍中苦杏仁苷的含量測定。
목적：건립측정지해정천환중고행인감함량적고효액상색보법。방법색보주위 Agela Venusil MP C18주（250 mm ×4.6 mm，5μm），류동상위갑순－수（20：80），류속위1.0 mL/min，검측파장위210 nm，주온실온。결과고행인감진양량재0.0530~0.8480μg （ r=0.9999）범위내여봉면적적분치구유량호선성관계，평균가양회수솔위98.64%，RSD위1.11%（ n=6）。결론해법간편、령민、준학가고，가용우지해정천환중고행인감적함량측정。
Objective To establish a HPLC method for the determination of the amygdalin content in Zhike Dingchuan Pills. Methods The Agela Venusil MP C18 column ( 250 mm × 4. 6 mm, 5 μm ) was used with the mobile phase of methanol-water ( 20 :80 ) , the flow rate was 1. 0 mL/min, the detection wavelength was 210 nm and the column temperature was the room temperature. Results The sam-ple size of amygdalin in the range of 0. 053 0-0. 848 0 μg ( r=0. 999 9 ) showed a good linear relationship with the peak area. The average recovery rate was 98. 64% with RSD=1. 10% ( n=6 ) . Conclusion This method is simple, sensitive and accurate, and can be used for the determination of the amygdalin content in Zhike Dingchuan Pills.
