CAJ | 학술논문

目的：建立同时测定大鼠血清中多索茶碱和甲泼尼龙质量浓度的反相高效液相色谱（RP－HPLC）法。方法以氢化可的松为内标物，色谱柱为Diamonsil C18柱（150mm ×4.6 mm，5μm），流动相为甲醇－0.5%醋酸溶液（54：46），检测波长260 nm，柱温30℃，流速1.0 mL/min，进样量20μL。结果多索茶碱、甲泼尼龙及血清内源性物质分离良好，多索茶碱质量浓度线性范围是0.5~40μg/mL （ r=0.9995），甲泼尼龙质量浓度线性范围是0.3~24μg/mL（ r=0.9996），两者日内和日间 RSD均小于10%（ n=5），平均回收率分别为93.49%( RSD=2.88%)和91.10%( RSD=2.56%)。结论该法简便、灵敏、准确，可用于多索茶碱和甲泼尼龙药代动力学、药物相互作用的研究和临床治疗药物监测。
목적：건립동시측정대서혈청중다색다감화갑발니룡질량농도적반상고효액상색보（RP－HPLC）법。방법이경화가적송위내표물，색보주위Diamonsil C18주（150mm ×4.6 mm，5μm），류동상위갑순－0.5%작산용액（54：46），검측파장260 nm，주온30℃，류속1.0 mL/min，진양량20μL。결과다색다감、갑발니룡급혈청내원성물질분리량호，다색다감질량농도선성범위시0.5~40μg/mL （ r=0.9995），갑발니룡질량농도선성범위시0.3~24μg/mL（ r=0.9996），량자일내화일간 RSD균소우10%（ n=5），평균회수솔분별위93.49%( RSD=2.88%)화91.10%( RSD=2.56%)。결론해법간편、령민、준학，가용우다색다감화갑발니룡약대동역학、약물상호작용적연구화림상치료약물감측。
Objective To establish a RP-HPLC method for the simultaneous determination of doxofylline and methylprednisolone con-tents in rat serum. Methods Hydrocortisone was selected as the internal standard. The chromatographic separation of analytes was achieved on the Diamonsil C18 column(150mm × 4. 6 mm,5 μm) at the temperature of 30 ℃, using the mobile phase consisting of methanol-0. 5% acetic acid solution ( 54 :46 ) at a flow rate of 1. 0 mL/min . The detection wavelength was set at 260 nm. Results Doxofylline, methylprednisolone and endogenous substances in serum were separated well. The linear ranges of doxofylline and methylpred-nisolone were 0. 5-40 μg/mL ( r=0. 999 5 ) and 0. 3-24 μg/mL ( r=0. 999 6 ) . The inter-day and intra-day RSD of doxofylline and methylprednisolone were all less than 10% ( n=5 ) . The average recovery rates were 93. 49% ( RSD=2. 88% ) for doxofylline and 91. 10%( RSD=2. 56%) for methylprednisolone. Conclusion This method can simultaneously determine the serum concentrations of dox-ofylline and methylprednisolone, which is convenient, sensitive, accurate and can be used to study the pharmacokinetic interaction and monitoring of serum drug concentration.