中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2015年
9期
10-11,12
,共3页
表没食子儿茶素没食子酸酯%食管鳞癌%细胞周期%流式细胞术
錶沒食子兒茶素沒食子痠酯%食管鱗癌%細胞週期%流式細胞術
표몰식자인다소몰식자산지%식관린암%세포주기%류식세포술
epigallocatechin-3-gallate%esophageal squamous cell carcinoma%cell cycle%flow cytometry
目的：探讨表没食子儿茶素没食子酸酯( epigallocatechin－3－ gallate，EGCG )抑制食管癌细胞Ec9706增殖作用及其相关机制。方法流式细胞术检测38例原发性食管鳞癌患者和20例患者正常食管黏膜组织的细胞周期及CDC25A蛋白表达水平。不同质量浓度EGCG (0，100，200，300 mg/L )作用Ec9706细胞24，48 h后流式细胞术检测细胞增殖及CDC25A蛋白表达水平。结果食管癌组织增殖指数为(24.92±11.01)%，显著高于正常食管组织的(14.49±3.83)%（ P＜0.05）；食管癌细胞中CDC25A蛋白表达水平显著高于食管正常组织中的表达水平（ P＜0.05）；不同质量浓度EGCG作用Ec9706细胞24 h后，细胞增殖指数及CDC25A蛋白表达水平显著降低（ P＜0.05）。结论 CDC25A在食管癌组织中高表达，且参与了食管癌组织高增殖状态的形成，EGCG可通过下调CDC25A表达，抑制食管癌细胞增殖。
目的：探討錶沒食子兒茶素沒食子痠酯( epigallocatechin－3－ gallate，EGCG )抑製食管癌細胞Ec9706增殖作用及其相關機製。方法流式細胞術檢測38例原髮性食管鱗癌患者和20例患者正常食管黏膜組織的細胞週期及CDC25A蛋白錶達水平。不同質量濃度EGCG (0，100，200，300 mg/L )作用Ec9706細胞24，48 h後流式細胞術檢測細胞增殖及CDC25A蛋白錶達水平。結果食管癌組織增殖指數為(24.92±11.01)%，顯著高于正常食管組織的(14.49±3.83)%（ P＜0.05）；食管癌細胞中CDC25A蛋白錶達水平顯著高于食管正常組織中的錶達水平（ P＜0.05）；不同質量濃度EGCG作用Ec9706細胞24 h後，細胞增殖指數及CDC25A蛋白錶達水平顯著降低（ P＜0.05）。結論 CDC25A在食管癌組織中高錶達，且參與瞭食管癌組織高增殖狀態的形成，EGCG可通過下調CDC25A錶達，抑製食管癌細胞增殖。
목적：탐토표몰식자인다소몰식자산지( epigallocatechin－3－ gallate，EGCG )억제식관암세포Ec9706증식작용급기상관궤제。방법류식세포술검측38례원발성식관린암환자화20례환자정상식관점막조직적세포주기급CDC25A단백표체수평。불동질량농도EGCG (0，100，200，300 mg/L )작용Ec9706세포24，48 h후류식세포술검측세포증식급CDC25A단백표체수평。결과식관암조직증식지수위(24.92±11.01)%，현저고우정상식관조직적(14.49±3.83)%（ P＜0.05）；식관암세포중CDC25A단백표체수평현저고우식관정상조직중적표체수평（ P＜0.05）；불동질량농도EGCG작용Ec9706세포24 h후，세포증식지수급CDC25A단백표체수평현저강저（ P＜0.05）。결론 CDC25A재식관암조직중고표체，차삼여료식관암조직고증식상태적형성，EGCG가통과하조CDC25A표체，억제식관암세포증식。
Objective To explore the effect and mechanism of epigallocatechin-3-gallate ( EGCG ) in inhibiting the proliferation action of esophageal carcinoma cells Ec9706. Methods The expression level of CDC25A protein and cell cycle were detected by the flow cy-tometry in 38 cases of esophageal squamous cell carcinoma and 20 cases of normal esophageal tissue. At 24, 48 h after treating the Ec9706 cells by different concentrations of EGCG ( 0, 100, 200, 300mg/L ) , the expression level of CDC25A and cell cycle were detect-ed by the flow cytometry. Results The proliferation index ( PI ) in the esophageal cancer tissue was ( 24. 92 ± 11. 01 ) %, which was significantly higher than ( 14. 49 ± 3. 83 )% in the normal esophageal tissue, the difference was statistically significant ( P < 0. 05 ) . The expression level of CDC25A protein in the esophageal carcinoma cells was significantly higher than that in the normal esophageal tissue with statistical difference between them ( P < 0. 05 ) . After 24 h different concentrations of EGCG acting on Ec9706 cells, PI and CDC25A protein expression level were decreased significantly, the difference was statistically significant ( P < 0. 05 ) . Conclusion The high expression of CDC25A in esophageal cancer tissue could participate in the formation of high proliferation status of esophageal can-cer tissue. EGCG could inhibit the proliferation of esophageal carcinoma cells by down-regulating the expression of CDC25A.
