CAJ | 학술논문

目的：探讨瑞舒伐他汀对大鼠脑缺血再灌注后白细胞介素－1β（ IL－1β）和白细胞介素－12（IL－12）的影响。方法大鼠随机分为3组，假手术组、脑缺血再灌注组（模型组）和瑞舒伐他汀组预处理组（实验组）。实验组在模型制作前用瑞舒伐他汀5 mg· kg－1· d－1连续灌胃10 d，1次／天。缺血1 h再灌注12 h后，进行神经功能评分，断头取脑，用酶联免疫吸附法和聚合酶链反应法检测大鼠的脑组织中IL－1β和IL－12的表达。结果与模型组相比，实验组大鼠神经功能评分显著降低，IL－1β和IL－12表达显著下调（均P＜0.05）。结论瑞舒伐他汀促进大鼠脑缺血再灌注损伤后神经功能的恢复，可能与抑制炎症因子的表达有关。
목적：탐토서서벌타정대대서뇌결혈재관주후백세포개소－1β（ IL－1β）화백세포개소－12（IL－12）적영향。방법대서수궤분위3조，가수술조、뇌결혈재관주조（모형조）화서서벌타정조예처리조（실험조）。실험조재모형제작전용서서벌타정5 mg· kg－1· d－1련속관위10 d，1차／천。결혈1 h재관주12 h후，진행신경공능평분，단두취뇌，용매련면역흡부법화취합매련반응법검측대서적뇌조직중IL－1β화IL－12적표체。결과여모형조상비，실험조대서신경공능평분현저강저，IL－1β화IL－12표체현저하조（균P＜0.05）。결론서서벌타정촉진대서뇌결혈재관주손상후신경공능적회복，가능여억제염증인자적표체유관。
Objective To observe the effects of rosuvastatin on the expression of interleukin-1β( IL -1β) and interleukin -12 ( IL-12 ) in rats with focal cerebral ischemia-reperfusion.Methods Sprague-Dawley rats were randomly assigned into three groups: Sham operation group, focal cerebral ischemia-reperfusion group(model group) and rosu-vastatin preconditioning group ( test group) .Before making a model, the rats in the test group were lavaged with rosuvastatin 5 mg· kg-1 · d-1 , once a day for consecutive days.The middle cerebral artery occlusion reperfu-sion model was made by the suture method ( ischemia for 1 hour, and reperfusion for 12 hours).The level of IL-1βand IL-12 expression were measured by enzyme -linked immunosorbent assay ( ELISA ) and polymerase chain reaction ( PCR ).Results Compared with model group, the expression of IL-1βand IL-12 in the rats of test group were downregulated ( P<0.05 ).Conclusion Rosuvastatin can relieve the brain damage induced by focal cerebral ischemia -reperfusion, whose mechanisms seemed related to inhibiting the expression of IL-1βand IL-12.