CAJ | 학술논문

目的：探讨糖尿病小鼠小肠上皮干细胞标志分子G蛋白偶联受体5（Lgr5）的表达及干细胞数量的改变情况。方法采用链脲佐菌素腹腔注射的方法建立糖尿病小鼠模型，用免疫组织化学检查（免疫组化）检测糖尿病小鼠（6只）与正常小鼠（6只）小肠上皮组织中 Lgr5的表达，并分离糖尿病小鼠与正常小鼠原代小肠上皮细胞，采用流式细胞技术测定 Lgr5阳性细胞在上皮细胞中的比例。结果免疫组化结果显示糖尿病与正常小鼠小肠上皮每个隐窝单位的 Lgr5阳性细胞数量分别为（3．7±0．5）个及（1．5±0．6）个，两者比较差异有统计学意义（P ＜0．05）。流式细胞技术测得糖尿病与正常小鼠小肠上皮 Lgr5阳性细胞比例分别为（28．75±3．69）％及（6．55±1．78）％，前者上皮组织中 Lgr5阳性细胞比例显著高于后者（P ＜0．05）。结论链脲佐菌素诱导的糖尿病小鼠小肠上皮 Lgr5阳性干细胞数量增加，Lgr5在糖尿病小鼠小肠上皮细胞高表达。
목적：탐토당뇨병소서소장상피간세포표지분자G단백우련수체5（Lgr5）적표체급간세포수량적개변정황。방법채용련뇨좌균소복강주사적방법건립당뇨병소서모형，용면역조직화학검사（면역조화）검측당뇨병소서（6지）여정상소서（6지）소장상피조직중 Lgr5적표체，병분리당뇨병소서여정상소서원대소장상피세포，채용류식세포기술측정 Lgr5양성세포재상피세포중적비례。결과면역조화결과현시당뇨병여정상소서소장상피매개은와단위적 Lgr5양성세포수량분별위（3．7±0．5）개급（1．5±0．6）개，량자비교차이유통계학의의（P ＜0．05）。류식세포기술측득당뇨병여정상소서소장상피 Lgr5양성세포비례분별위（28．75±3．69）％급（6．55±1．78）％，전자상피조직중 Lgr5양성세포비례현저고우후자（P ＜0．05）。결론련뇨좌균소유도적당뇨병소서소장상피 Lgr5양성간세포수량증가，Lgr5재당뇨병소서소장상피세포고표체。
Objective To measure the expression of the stem cell marker leucine-rich repeat-contai-ning G protein-coupled receptor 5 (Lgr5)in the small intestinal epithelium and investigate the changes in the quantity of stem cells in the diabetic mice.Methods Diabetic mouse model was established by intraperitoneal injection of streptozotocin (STZ).The expression levels of Lgr5 in small intestinal epithelial tissues of the dia-betic (n =6)and normal mice (n =6)were assessed by immunohistochemistry.Primary intestinal epithelial cells were isolated from the diabetic and normal mice and the percentage of Lgr5-positive cells among epithelial cells were assessed by flow cytometry.Results Immunohistochemical analysis revealed that the quantity of Lgr5-positive cells per crypt in the diabetic and normal mice was (3.7 ±0.5)and (1.5 ±0.6)with statistical significance (P <0.05).Flow cytometry demonstrated that the percentage of Lgr5-positive cells in the intesti-nal epithelial cells of the diabetic mice was (28.75 ±3.69)%,significantly higher compared with (6.55 ± 1.78)% of the normal mice (P <0.05).Conclusions The quantity of Lgr5-positive stem cells in the small intestinal epithelium of the STZ-induced diabetic mice was increased.Lgr5 was highly expressed in the small intestinal epithelium of the diabetic mice.