CAJ | 학술논문

目的探讨B细胞幼淋巴细胞白血病(B-PLL)免疫表型特征.方法通过多参数流式细胞术检测并回顾性分析7例B-PLL患者免疫表型,包括CD5、CD10、CD19、CD20、CD22、CD23、CD25、CD27、CD38、CD45、CD148、CD200、ZAP-70、FMC-7、κ、λ.结果所有患者泛B细胞标志CD19、CD20、CD22均为阳性,其中仅有1例CD20呈中等强度表达,其余6例CD20均呈强阳性表达,CD22仅1例表达较弱,其余6例均呈中等强度表达.所有患者CD10均为阴性,4例CD5阳性,6例CD23阳性,2例FMC-7阳性,1例CD38阳性,4例CD200阳性,CD148均为阳性,轻链限制性表达检测中3例κ型,4例λ型.根据Matutes慢性淋巴细胞白血病免疫表型积分系统评分,1分1例,2、3分各3例.结论 B-PLL的诊断需要整合临床表现、外周血细胞形态学、免疫表型、细胞遗传学、分子生物学等结果综合判断,CD148、CD200或许可以成为B-PLL辅助诊断标志,B-PLL的免疫表型分布特征仍需要增加样本量进一步探讨.
목적 탐토B세포유림파세포백혈병(B-PLL)면역표형특정.방법 통과다삼수류식세포술검측병회고성분석7례B-PLL환자면역표형,포괄CD5、CD10、CD19、CD20、CD22、CD23、CD25、CD27、CD38、CD45、CD148、CD200、ZAP-70、FMC-7、κ、λ.결과 소유환자범B세포표지CD19、CD20、CD22균위양성,기중부유1례CD20정중등강도표체,기여6례CD20균정강양성표체,CD22부1례표체교약,기여6례균정중등강도표체.소유환자CD10균위음성,4례CD5양성,6례CD23양성,2례FMC-7양성,1례CD38양성,4례CD200양성,CD148균위양성,경련한제성표체검측중3례κ형,4례λ형.근거Matutes만성림파세포백혈병면역표형적분계통평분,1분1례,2、3분각3례.결론 B-PLL적진단수요정합림상표현、외주혈세포형태학、면역표형、세포유전학、분자생물학등결과종합판단,CD148、CD200혹허가이성위B-PLL보조진단표지,B-PLL적면역표형분포특정잉수요증가양본량진일보탐토.
Objective To explore the immunophenotypic characteristics of the B-cell prolymphocytic leukemia (B-PLL).Methods 4-color multiparameter flow cytometry and a panel of monoclonal antibodies were used to detect immunophenotype in 7 cases of B-PLL including CD5,CD10,CD19,CD20,CD22,CD23,CD25,CD27,CD38,CD45,CD148,CD200,ZAP-70,FMC-7,κ and λ.Results Among 7 cases,all expressed pan-B-cell markers CD19,CD20 and CD22.CD20 was bright positive except one case.CD22 expression pattern was similar to CD20,and only one case expressed weakly,others were medium positive.All cases were negative for CD10 while positive for CD148,CD5,CD23,FMC-7,CD38 and CD200 was positive in 4,6,2,1 and 4 cases,respectively.Light chain restriction expression was observed in 3 cases of κ type and 4 cases of λ type.According to Matutes chronic lymphocytic leukemia scoring system,one case was 1-point score,3 cases were 2-point score,and 3 cases were 3-point score.Conclusions The diagnosis of B-PLL needs systemic integration of clinical manifestations,morphology,cytogenetics,molecular biology and other results.CD148 and CD200 may be the novel differential diagnostic markers of B-PLL.It needs a large sample size of B-PLL patients to furtherly explore the characteristics of immunophenotype of B-PLL.