白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2015年
4期
217-219
,共3页
胡蓉华%郭轶先%刘聪艳%张维%万岁桂%苏力%夏长青
鬍蓉華%郭軼先%劉聰豔%張維%萬歲桂%囌力%夏長青
호용화%곽질선%류총염%장유%만세계%소력%하장청
急性白血病%中期因子%WT1%酶联免疫吸附测定
急性白血病%中期因子%WT1%酶聯免疫吸附測定
급성백혈병%중기인자%WT1%매련면역흡부측정
Acute leukemia%Midkine%WT1 gene%Enzyme-linked immunosorbent assay
目的 探讨血清中期因子(MK)水平在急性白血病(AL)不同发展阶段的意义及其与WT1基因的关系.方法 用酶联免疫吸附实验(ELISA)检测86例初治和完全缓解的AL患者组及30名健康对照血清中MK水平;用实时定量PCR法检测其中15例初治急性髓系白血病(AML)患者骨髓细胞中WT1表达量.结果 初治组血清MK水平(中位数及四分位数)为7.52 ng/ml[(5.44,10.55)ng/ml],缓解组MK水平为3.52 ng/ml[(1.56,5.20) ng/ml],健康对照组MK水平为2.44 ng/ml[(1.89,3.12)ng/ml],初治组较缓解组和健康对照组水平均升高,差异有统计学意义(P＜0.01),缓解组和健康对照组间MK水平差异无统计学意义(P＞0.05).初治B细胞急性淋巴细胞白血病(B-ALL)组血清MK水平为7.88 ng/ ml[(5.78,15.78) ng/ml],初治AML中MK水平为6.25 ng/ml[(4.59,16.33) ng/ml],两组间差异无统计学意义(P＞0.05);血清MK水平与骨髓中WT1基因表达量间存在相关性(r=0.529,P=0.043).结论 初治AL患者血清中MK表达水平升高,完全缓解后MK表达水平下降,ELISA法测定血清MK可以作为AL患者病情评价的手段之一;MK与WT1在基因定位上相邻,临床意义相近,并且血清MK和WT1基因在定量上存在相关性.
目的 探討血清中期因子(MK)水平在急性白血病(AL)不同髮展階段的意義及其與WT1基因的關繫.方法 用酶聯免疫吸附實驗(ELISA)檢測86例初治和完全緩解的AL患者組及30名健康對照血清中MK水平;用實時定量PCR法檢測其中15例初治急性髓繫白血病(AML)患者骨髓細胞中WT1錶達量.結果 初治組血清MK水平(中位數及四分位數)為7.52 ng/ml[(5.44,10.55)ng/ml],緩解組MK水平為3.52 ng/ml[(1.56,5.20) ng/ml],健康對照組MK水平為2.44 ng/ml[(1.89,3.12)ng/ml],初治組較緩解組和健康對照組水平均升高,差異有統計學意義(P＜0.01),緩解組和健康對照組間MK水平差異無統計學意義(P＞0.05).初治B細胞急性淋巴細胞白血病(B-ALL)組血清MK水平為7.88 ng/ ml[(5.78,15.78) ng/ml],初治AML中MK水平為6.25 ng/ml[(4.59,16.33) ng/ml],兩組間差異無統計學意義(P＞0.05);血清MK水平與骨髓中WT1基因錶達量間存在相關性(r=0.529,P=0.043).結論 初治AL患者血清中MK錶達水平升高,完全緩解後MK錶達水平下降,ELISA法測定血清MK可以作為AL患者病情評價的手段之一;MK與WT1在基因定位上相鄰,臨床意義相近,併且血清MK和WT1基因在定量上存在相關性.
목적 탐토혈청중기인자(MK)수평재급성백혈병(AL)불동발전계단적의의급기여WT1기인적관계.방법 용매련면역흡부실험(ELISA)검측86례초치화완전완해적AL환자조급30명건강대조혈청중MK수평;용실시정량PCR법검측기중15례초치급성수계백혈병(AML)환자골수세포중WT1표체량.결과 초치조혈청MK수평(중위수급사분위수)위7.52 ng/ml[(5.44,10.55)ng/ml],완해조MK수평위3.52 ng/ml[(1.56,5.20) ng/ml],건강대조조MK수평위2.44 ng/ml[(1.89,3.12)ng/ml],초치조교완해조화건강대조조수평균승고,차이유통계학의의(P＜0.01),완해조화건강대조조간MK수평차이무통계학의의(P＞0.05).초치B세포급성림파세포백혈병(B-ALL)조혈청MK수평위7.88 ng/ ml[(5.78,15.78) ng/ml],초치AML중MK수평위6.25 ng/ml[(4.59,16.33) ng/ml],량조간차이무통계학의의(P＞0.05);혈청MK수평여골수중WT1기인표체량간존재상관성(r=0.529,P=0.043).결론 초치AL환자혈청중MK표체수평승고,완전완해후MK표체수평하강,ELISA법측정혈청MK가이작위AL환자병정평개적수단지일;MK여WT1재기인정위상상린,림상의의상근,병차혈청MK화WT1기인재정량상존재상관성.
Objective To explore the meaning of midkine (MK) levels in serum in different development stage of acute leukemia,and to explore the relationship between MK and WT1.Methods The levels of the MK in serum of 86 cases of acute leukemia and 30 cases of normal people were detected by ELISA.Real-time quantitative PCR (RQ-PCR) method was used to determine the expression of WT1 at mRNA level in 15 AML patients.Results The MK level in serum in the new diagnosed group was higher than that in the complete remission group and the normal control group [7.52 (5.44,10.55) ng/ml vs 3.52 (1.56,5.20) ng/ml vs 2.44 (1.89,3.12) ng/ml].There' s no statistical difference between midkine level in new diagnosed acute B cell leukemia (B-ALL) group and acute myeloid leukemia (AML) group [7.88 (5.78,15.78) ng/ml vs 6.25 (4.59,16.33) ng/ml].The clear correlation was found between the level of serum MK and quantities of marrow WT1 gene (r =0.529,P =0.043).Conclusions The level of MK in serum of acute leukemia patients is increased at the time of new diagnosis and decreased at complete remission.ELISA may be a way to measure the status of AL.The location of MK gene is adjacent to WT1 gene and MK' s clinical significance is similar to WT1' s,furthermore,there is a clear correlation between MK in serum and WT1 of marrow in quantities.
