CAJ | 학술논문

草甘膦抗性是当代农业重要的农艺性状。为了选择最佳的抗性基因用于植物筛选标记，通过选取 cp4-epsps 、GR79-epsps 、gat4621和 GAT 四个草甘膦抗性基因进行比较，在拟南芥中验证各基因对草甘膦的抗性。4个基因的转化苗经4个浓度的草甘膦喷施检测，结果显示，GAT 类基因的抗性明显高于epsps 类基因，基因间的差异达到了极显著水平。gat4621基因对草甘膦的抗性最好，在各浓度下存活率均表现为最高值，且抗性稳定。国内发掘的 GAT 基因对草甘膦也表现出良好的抗性。GAT 类基因的拟南芥转化苗在喷施高浓度草甘膦条件下出现严重抽苔抑制；但在停施草甘膦后抑制解除，且可以开花结实。转 epsps 类基因可以获得高抗草甘膦植株，但在花期喷施草甘膦将造成败育。从基因大小及抗性强弱考虑，GAT 类基因作为筛选标记基因应该更为适合。
초감련항성시당대농업중요적농예성상。위료선택최가적항성기인용우식물사선표기，통과선취 cp4-epsps 、GR79-epsps 、gat4621화 GAT 사개초감련항성기인진행비교，재의남개중험증각기인대초감련적항성。4개기인적전화묘경4개농도적초감련분시검측，결과현시，GAT 류기인적항성명현고우epsps 류기인，기인간적차이체도료겁현저수평。gat4621기인대초감련적항성최호，재각농도하존활솔균표현위최고치，차항성은정。국내발굴적 GAT 기인대초감련야표현출량호적항성。GAT 류기인적의남개전화묘재분시고농도초감련조건하출현엄중추태억제；단재정시초감련후억제해제，차가이개화결실。전 epsps 류기인가이획득고항초감련식주，단재화기분시초감련장조성패육。종기인대소급항성강약고필，GAT 류기인작위사선표기기인응해경위괄합。
Glyphosate tolerance is an important agronomic trait in modern agriculture.In order to determine the best glyphosate tolerance gene for plant selectable markers we compared four glyphosate tolerance genes,cp4-epsps ,GR79-epsps ,gat4621 and GAT,in Arabidopsis .Four transgenic plants harboring each gene were trea-ted with four different rates of glyphosate.The results showed that the resistance of plants with GAT gene was significantly higher than plants with the epsps gene.Plants with the gat4621 gene had the highest survival rate at all herbicide rates.The plants with the domestic GAT gene also had strong resistance.Reproductive devel-opment of Arabidopsis plants with the GAT gene was suppressed at higher glyphosate application rates.It was concluded that the GAT gene was more suitable as a selectable marker gene.